Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
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Genus and species- specific identification of mycoplasmas by 16s rrna amplification
F.J.M. van Kuppeveld,J. T. M. Van Der Logt,A.F. Angulo,M.J. van Zoest,W.G.V. Quint,Hubert G. M. Niesters,Jochem M. D. Galama,Willem J. G. Melchers +7 more
TL;DR: Systematic computer alignment of mycoplasmal 16S rRNA sequences allowed the identification of variable regions with both genus- and species-specific sequences, and Mycoplasma collis proved to be species specific in the polymerase chain reaction.
Journal ArticleDOI
Contamination and Sensitivity Issues with a Real-Time Universal 16S rRNA PCR
Caroline E. Corless,Malcolm Guiver,Ray Borrow,Valerie Edwards-Jones,Edward B. Kaczmarski,Andrew J. Fox +5 more
TL;DR: A set of universal oligonucleotide primers specific for the conserved regions of the eubacterial 16S rRNA gene was designed for use with the real-time PCR Applied Biosystems 7700 (TaqMan) system, but problems were noted with the use of this gene as an amplification target.
Journal ArticleDOI
Rochalimaea henselae Infection A New Zoonosis With the Domestic Cat as Reservoir
TL;DR: It is documented that the domestic cat serves as a major persistent reservoir for R henselae, with prolonged, asymptomatic bacteremia from which humans, especially the immunocompromised, may acquire potentially serious infections.
Journal ArticleDOI
Infection with Hepatitis GB Virus C in Patients on Maintenance Hemodialysis
Kazuo Masuko,Takehiro Mitsui,Keiko Iwano,Chikao Yamazaki,Kenji Okuda,Teruo Meguro,Naoki Murayama,Taisuke Inoue,Fumio Tsuda,Hiroaki Okamoto,Yuzo Miyakawa,Makoto Mayumi +11 more
TL;DR: Patients on maintenance hemodialysis are at increased risk for HGBV-C infection, which produces persistent infections, which may be transmitted by transfusions but may also been transmitted by other means.
Journal ArticleDOI
A hepatitis B virus mutant associated with an epidemic of fulminant hepatitis
TL;DR: The strain of hepatitis B virus that caused this epidemic was examined, and a mutant hepatitis B viral strain was transmitted from a common source to five patients who subsequently died of fulminant hepatitis B infection.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.