Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
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Detection of hepatitis B virus DNA in serum samples via nested PCR and MALDI-TOF mass spectrometry
Christian Jurinke,Bernhard Zöllner,Heinz-Hubert Feucht,A. Jacob,J. Kirchhübel,A. Lüchow,D. van den Boom,Rainer Laufs,Hubert Köster +8 more
TL;DR: The detection strategy introduced here has a high potential for automation and represents a fast and reliable method of detection for HBV DNA in serum without the need for time consuming gel electrophoresis and labeling or hybridization procedures.
Journal ArticleDOI
Strategies for diagnosis of HTLV‐I and ‐II
TL;DR: The aim of thus study was to compare the accuracy of a combination of two sensitive ELISAs with Western blot, a line immunoassay, and PCR for diagnosis of HTLV infection.
Journal ArticleDOI
Naturally occurring hepatitis B virus core gene mutations
Ulus Salih Akarca,Anna S. Lok +1 more
TL;DR: Sera from 69 Chinese patients with chronic HBV infection were analyzed by direct sequencing of polymerase chain reaction amplification of HBV DNA to determine the frequency and location of naturally occurring HBV core gene mutations.
Journal ArticleDOI
Diagnosis of congenital cytomegalovirus infection by detection of viral DNA in dried blood spots.
TL;DR: The method of CMV DNA extraction in medium followed by amplification of the gp58 region showed 100% sensitivity and specificity compared with isolation in cell culture.
Journal ArticleDOI
Improved detection of rotavirus shedding by polymerase chain reaction.
TL;DR: To improve identification of children excreting rotavirus a method for the amplification of rotav virus RNA by the polymerase chain reaction (PCR) was developed and was compared with a solid-phase enzyme immunoassay in the detection ofRotavirus shedding by infants in hospital during the winter peak of rotvirus infections.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.