Building Biological Flashlights: Orthogonal Luciferases and Luciferins for in Vivo Imaging.
Reads0
Chats0
TLDR
The goal was to generate multiple orthogonal luciferases that are responsive to unique scaffolds and could be used concurrently in living animals, and to develop Orthogonal bioluminescent probes that can selectively process luciferin analogs to produce light.Abstract:
ConspectusBioluminescence is widely used for real-time imaging in living organisms. This technology features a light-emitting reaction between enzymes (luciferases) and small molecule substrates (l...read more
Citations
More filters
Journal ArticleDOI
Applications of bioluminescence in biotechnology and beyond.
Aisha J. Syed,James C. Anderson +1 more
TL;DR: Bioluminescence is the natural phenomenon by which living creatures produce light as mentioned in this paper, which occurs when the oxidation of a small-molecule luciferin is catalysed by an enzyme luciferase to form an excited-state species that emits light.
Journal ArticleDOI
Bright Chromenylium Polymethine Dyes Enable Fast, Four-Color In Vivo Imaging with Shortwave Infrared Detection.
Emily D. Cosco,Bernardo Assein Arús,Anthony L. Spearman,Timothy L. Atallah,Irene Lim,Olivia S Leland,Justin R. Caram,Thomas S. Bischof,Oliver T. Bruns,Ellen M. Sletten +9 more
TL;DR: In this article, the authors explored derivatives with functional group substitution at the 2-position, deemed chromenylium polymethine dyes, which reduced nonradiative rates and enhanced emissive properties, enabling non-invasive imaging in mice in a single color at 300 fps and in three colors at 100 fps.
Journal ArticleDOI
Seeing (and Using) the Light: Recent Developments in Bioluminescence Technology.
TL;DR: This review highlights how bioluminescent systems are being leveraged not just for sensing-but also controlling-biological processes, and showcases how new luciferins and engineered luciferases are expanding the scope of optical imaging.
Journal ArticleDOI
Bioluminescence Profiling of NanoKAZ/NanoLuc Luciferase Using a Chemical Library of Coelenterazine Analogues
Eloi P. Coutant,Glwadys Gagnot,Glwadys Gagnot,Vincent Hervin,Racha Baatallah,Sophie Goyard,Yves Jacob,Thierry Rose,Yves L. Janin +8 more
TL;DR: An extensive structure-bioluminescence relationship study of a chemical library of analogues of coelenterazine, using nanoKAZ/NanoLuc, a mutated luciferase originated from the catalytic subunit of the deep-sea shrimp Oplophorus gracilirostris is described.
Journal ArticleDOI
Multicomponent Bioluminescence Imaging with a π-Extended Luciferin
TL;DR: This work provides the first example of Rosetta-guided design in engineering bioluminescent tools and expands the scope of orthogonal imaging probes.
References
More filters
Journal ArticleDOI
Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate.
Mary P. Hall,James Unch,Brock Binkowski,Michael P. Valley,Braeden L. Butler,Monika G. Wood,Paul Otto,Kristopher Zimmerman,Gediminas Vidugiris,Thomas Machleidt,Matthew B. Robers,Hélène A Benink,Christopher T. Eggers,Michael R. Slater,Poncho Meisenheimer,Dieter Klaubert,Frank Fan,Lance P. Encell,Keith V. Wood +18 more
TL;DR: A novel bioluminescence system capable of more efficient light emission with superior biochemical and physical characteristics is engineered in mammalian cells by merging optimization of protein structure with development of a novel imidazopyrazinone substrate (furimazine).
Journal ArticleDOI
Visualizing gene expression in living mammals using a bioluminescent reporter.
Christopher H. Contag,Stanley D. Spilman,Pamela R. Contag,Masafumi Oshiro,Brian Eames,Phyllis A. Dennery,David K. Stevenson,David A. Benaron +7 more
TL;DR: It is concluded that gene regulation, DNA delivery and expression can now be noninvasively monitored in living mammals using a luciferase reporter, and real‐time, noninvasive study of gene expression in living animal models for human development and disease is possible.
Journal ArticleDOI
Structural basis for the spectral difference in luciferase bioluminescence.
Toru Nakatsu,Susumu Ichiyama,Jun Hiratake,Adrian Saldanha,Nobuyuki Kobashi,Kanzo Sakata,Hiroaki Kato +6 more
TL;DR: The results indicate that the degree of molecular rigidity of the excited state of oxyluciferin, which is controlled by a transient movement of Ile 288, determines the colour of bioluminescence during the emission reaction.
Journal ArticleDOI
Evolutionary information for specifying a protein fold.
Michael Socolich,Michael Socolich,Steve W. Lockless,Steve W. Lockless,Steve W. Lockless,William P. Russ,Heather Lee,Heather Lee,Kevin H. Gardner,Rama Ranganathan +9 more
TL;DR: This work attempts to define the sequence rules for specifying a protein fold by computationally creating artificial protein sequences using only statistical information encoded in a multiple sequence alignment and no tertiary structure information.
Journal ArticleDOI
Bioluminescent Assays for High-Throughput Screening
Frank Fan,Keith V. Wood +1 more
TL;DR: This review addresses recent progress made in the use of bioluminescent assays for HTS, highlighting new detection capabilities brought about by engineering luciferase genes, enzymes, and substrates.
Related Papers (5)
Single-cell bioluminescence imaging of deep tissue in freely moving animals
Satoshi Iwano,Mayu Sugiyama,Hiroshi Hama,Akiya Watakabe,Naomi Hasegawa,Takahiro Kuchimaru,Kazumasa Z. Tanaka,Megumu Takahashi,Yoko Ishida,Jun-ichi Hata,Satoshi Shimozono,Kana Namiki,Takashi Fukano,Masahiro Kiyama,Hideyuki Okano,Shinae Kizaka-Kondoh,Thomas J. McHugh,Tetsuo Yamamori,Hiroyuki Hioki,Shojiro Maki,Atsushi Miyawaki +20 more