Seeing (and Using) the Light: Recent Developments in Bioluminescence Technology.
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TLDR
This review highlights how bioluminescent systems are being leveraged not just for sensing-but also controlling-biological processes, and showcases how new luciferins and engineered luciferases are expanding the scope of optical imaging.About:
This article is published in Chemistry & Biology.The article was published on 2020-08-20 and is currently open access. It has received 52 citations till now. The article focuses on the topics: Bioluminescence.read more
Citations
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A light- and calcium-gated transcription factor for imaging and manipulating activated neurons
Craig P. Wildes,Mateo I. Sánchez,Gordon F. Glober,Gillian A. Matthews,Kay M. Tye,Wenjing Wang,Tanyaporn Pattarabanjird,Alice Y. Ting +7 more
TL;DR: FLARE is an engineered transcription factor that drives expression of fluorescent proteins, opsins, and other genetically encoded tools only in the subset of neurons that experienced activity during a user-defined time window and should be useful for the study of activity-dependent processes in neurons and other cells that signal with calcium.
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Applications of bioluminescence in biotechnology and beyond.
Aisha J. Syed,James C. Anderson +1 more
TL;DR: Bioluminescence is the natural phenomenon by which living creatures produce light as mentioned in this paper, which occurs when the oxidation of a small-molecule luciferin is catalysed by an enzyme luciferase to form an excited-state species that emits light.
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Bioorthogonal Reactions of Triarylphosphines and Related Analogues.
TL;DR: A review of recent advances in the field of bioorthogonal phosphines can be found in this paper, where the Staudinger ligation also paved the way for the development of other phosphorus-based chemistries, many of which are widely employed in biological experiments.
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Emerging tools for bioluminescence imaging
TL;DR: A compendium of the latest developments (2018-2020) and their notable applications can be found in this paper, where the authors evaluate the emerging tools for Bioluminescence imaging aiming to provide the reader with an updated compendium.
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De novo design of luciferases using deep learning
Andy Hsien-Wei Yeh,Christoffer Norn,Ya. I. Kipnis,Doug Tischer,S.J. Pellock,Declan Marshall Evans,Pengchen Ma,Gyu Rie Lee,Jason Z. Zhang,Ivan Anishchenko,Brian Coventry,Longxing Cao,Justas Dauparas,Samer Halabiya,Michelle DeWitt,Lauren Carter,K. N. Houk,D. Baker +17 more
TL;DR: In this article , a deep-learning-based hallucination approach was used to generate large numbers of idealized protein structures containing diverse pocket shapes and designed sequences that encode them, which can be used to design artificial luciferases that selectively catalyse the oxidative chemiluminescence of the synthetic luciferin substrates diphenylterazine 3 and 2-deoxycoelenterazine.
References
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Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
Nathan C. Shaner,Robert E. Campbell,Robert E. Campbell,Paul Steinbach,Ben N G Giepmans,Amy E. Palmer,Roger Y. Tsien +6 more
TL;DR: The latest red version matures more completely, is more tolerant of N-terminal fusions and is over tenfold more photostable than mRFP1, and three monomers with distinguishable hues from yellow-orange to red-orange have higher quantum efficiencies.
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Millisecond-timescale, genetically targeted optical control of neural activity.
TL;DR: In this paper, the authors adapted the naturally occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel, by using lentiviral gene delivery in combination with high-speed optical switching to photostimulate mammalian neurons.
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Genes that mediate breast cancer metastasis to lung
Andy J. Minn,Gaorav P. Gupta,Peter M. Siegel,Peter M. Siegel,Paula D. Bos,Weiping Shu,Dilip Giri,Dilip Giri,Agnes Viale,Adam B. Olshen,William L. Gerald,Joan Massagué +11 more
TL;DR: A set of genes are identified that marks and mediates breast cancer metastasis to the lungs and serve dual functions, providing growth advantages both in the primary tumour and in the lung microenvironment.
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Extraction, Purification and Properties of Aequorin, a Bioluminescent Protein from the Luminous Hydromedusan, Aequorea
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Wavelength mutations and posttranslational autoxidation of green fluorescent protein
TL;DR: The availability of two visibly distinct colors should significantly extend the usefulness of GFP in molecular and cell biology by enabling in vivo visualization of differential gene expression and protein localization and measurement of protein association by fluorescence resonance energy transfer.
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