Changes in circulating microRNA levels associated with prostate cancer
Richard J. Bryant,Traci Pawlowski,James W.F. Catto,G Marsden,Robert L. Vessella,Brian Kent Rhees,C. Kuslich,Tapio Visakorpi,Freddie C. Hamdy +8 more
TLDR
Observations suggest that changes in miR concentration in prostate cancer patients may be identified by analysing various body fluids, and circulating miRs may be used to diagnose and stage prostate cancer.Abstract:
BACKGROUND: The aim of this study was to investigate the hypothesis that changes in circulating microRNAs (miRs) represent potentially useful biomarkers for the diagnosis, staging and prediction of outcome in prostate cancer. METHODS: Real-time polymerase chain reaction analysis of 742 miRs was performed using plasma-derived circulating microvesicles of 78 prostate cancer patients and 28 normal control individuals to identify differentially quantified miRs. RESULTS: A total of 11 miRs were differentially quantified in prostate cancer patients compared with controls, including 9 in patients without metastases. In all, 16 miRs were present in significantly greater amounts in prostate cancer patients with metastases compared with those without metastases. The association of miR-141 and miR-375 with metastatic prostate cancer was confirmed using serum-derived exosomes and microvesicles in a separate cohort of patients with recurrent or non-recurrent disease following radical prostatectomy. An analysis of five selected miRs in urine samples found that miR-107 and miR-574-3p were quantified at significantly higher concentrations in the urine of men with prostate cancer compared with controls. CONCLUSION: These observations suggest that changes in miR concentration in prostate cancer patients may be identified by analysing various body fluids. Moreover, circulating miRs may be used to diagnose and stage prostate cancer.read more
Citations
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Extracellular Vesicles in Cancer: Cell-to-Cell Mediators of Metastasis
Annette Becker,Basant Kumar Thakur,Joshua Mitchell Weiss,Han Sang Kim,Han Sang Kim,Héctor Peinado,David Lyden +6 more
TL;DR: Clinically, EVs may be biomarkers and novel therapeutic targets for cancer progression, particularly for predicting and preventing future metastatic development.
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Absolute quantification by droplet digital PCR versus analog real-time PCR
Christopher Hindson,John R. Chevillet,Hilary A. Briggs,Emily N. Gallichotte,Ingrid K. Ruf,Benjamin J. Hindson,Robert L. Vessella,Muneesh Tewari +7 more
TL;DR: A comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision and improved day-to-day reproducibility of dd PCR but with comparable sensitivity, which translated to superior diagnostic performance for identifying individuals with cancer.
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Characterization of human plasma-derived exosomal RNAs by deep sequencing
Xiaoyi Huang,Tiezheng Yuan,Michael Tschannen,Zhifu Sun,Howard J. Jacob,Meijun Du,Meihua Liang,Rachel L. Dittmar,Yong Liu,Mingyu Liang,Manish Kohli,Stephen N. Thibodeau,Lisa A. Boardman,Liang Wang +13 more
TL;DR: This study demonstrated that a wide variety of RNA species are embedded in the circulating vesicles and suggested that the highly abundant miRNAs may play an important role in biological functions such as protein phosphorylation, RNA splicing, chromosomal abnormality, and angiogenesis.
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Clinical relevance of circulating cell-free microRNAs in cancer
TL;DR: The latest developments in the use of circulating microRNAs as prognostic and predictive biomarkers are considered and their utility in personalized medicine is discussed.
Journal ArticleDOI
Quantitative and stoichiometric analysis of the microRNA content of exosomes.
John R. Chevillet,Qing Kang,Ingrid K. Ruf,Hilary A. Briggs,Lucia Vojtech,Sean M. Hughes,Heather H. Cheng,Jason D. Arroyo,Emily K. Meredith,Emily N. Gallichotte,Era L. Pogosova-Agadjanyan,Colm Morrissey,Derek L. Stirewalt,Florian Hladik,Evan Y. Yu,Celestia S. Higano,Muneesh Tewari +16 more
TL;DR: It is shown here that most exosomes derived from standard preparations do not harbor many copies of miRNA molecules, and are, therefore, individually unlikely to be functional as vehicles for miRNA-based communication.
References
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Journal ArticleDOI
Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
TL;DR: It is shown here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity and established the measurement of tumor-derived mi RNAs in serum or plasma as an important approach for the blood-based detection of human cancer.
Journal ArticleDOI
Causes and consequences of microRNA dysregulation in cancer
TL;DR: Because malignant cells show dependence on the dysregulated expression of miRNA genes, which in turn control or are controlled by the dysregulation of multiple protein-coding oncogenes or tumour suppressor genes, these small RNAs provide important opportunities for the development of future miRNA-based therapies.
Journal ArticleDOI
The Influence of Finasteride on the Development of Prostate Cancer
Ian M. Thompson,Phyllis J. Goodman,Catherine M. Tangen,M. Scott Lucia,Gary J. Miller,Leslie G. Ford,Michael M. Lieber,R. Duane Cespedes,JN Atkins,Scott M. Lippman,Susie M. Carlin,Anne Ryan,Connie M. Szczepanek,John Crowley,Charles A. Coltman +14 more
TL;DR: Finasteride prevents or delays the appearance of prostate cancer, but this possible benefit and a reduced risk of urinary problems must be weighed against sexual side effects and the increased risk of high-grade prostate cancer.
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