Characterization of florfenicol resistance genes in the coagulase-negative Staphylococcus (CoNS) isolates and genomic features of a multidrug-resistant Staphylococcus lentus strain H29.
Chongyang Wu,Chongyang Wu,Xueya Zhang,Jialei Liang,Qiaoling Li,Hailong Lin,Chaoqing Lin,Hongmao Liu,Danying Zhou,Wei Lu,Zhewei Sun,Xi Lin,Hailin Zhang,Keiwei Li,Teng Xu,Qiyu Bao,Junwan Lu +16 more
Reads0
Chats0
TLDR
In this paper, the authors analyzed the distribution of florfenicol resistance genes in 39 CoNS strains isolated from poultry and livestock animals and seafood and found that the isolates from the animals showed a higher resistance rate (23/28, 82.1%) and much higher inhibitory concentration (MIC) levels than those from seafood.Abstract:
With the wide use of florfenicol to prevent and treat the bacterial infection of domestic animals, the emergence of the florfenicol resistance bacteria is increasingly serious. It is very important to elucidate the molecular mechanism of the bacteria’s resistance to florfenicol. The minimum inhibitory concentration (MIC) levels were determined by the agar dilution method, and polymerase chain reaction was conducted to analyze the distribution of florfenicol resistance genes in 39 CoNS strains isolated from poultry and livestock animals and seafood. The whole genome sequence of one multidrug resistant strain, Staphylococcus lentus H29, was characterized, and comparative genomics analysis of the resistance gene-related sequences was also performed. As a result, the isolates from the animals showed a higher resistance rate (23/28, 82.1%) and much higher MIC levels to florfenicol than those from seafood. Twenty-seven animal isolates carried 37 florfenicol resistance genes (including 26 fexA, 6 cfr and 5 fexB genes) with one carrying a cfr gene, 16 each harboring a fexA gene, 5 with both a fexA gene and a fexB gene and the other 5 with both a fexA gene and a cfr gene. On the other hand, all 11 isolates from seafood were sensitive to florfenicol, and only 3 carried a fexA gene each. The whole genome sequence of S. lentus H29 was composed of a chromosome and two plasmids (pH29-46, pH29-26) and harbored 11 resistance genes, including 6 genes [cfr, fexA, ant(6)-Ia, aacA-aphD, mecA and mph(C)] encoded on the chromosome, 4 genes [cfr, fexA, aacA-aphD and tcaA] on pH29-46 and 1 gene (fosD) on pH29-26. We found that the S. lentus H29 genome carried two identical copies of the gene arrays of radC-tnpABC-hp-fexA (5671 bp) and IS256-cfr (2690 bp), of which one copy of the two gene arrays was encoded on plasmid pH29-46, while the other was encoded on the chromosome. The current study revealed the wide distribution of florfenicol resistance genes (cfr, fexA and fexB) in animal bacteria, and to the best of our knowledge, this is the first report that one S. lentus strain carried two identical copies of florfenicol resistance-related gene arrays.read more
Citations
More filters
Journal ArticleDOI
Mobile Oxazolidinone Resistance Genes in Gram-Positive and Gram-Negative Bacteria.
Stefan Schwarz,Stefan Schwarz,Wanjiang Zhang,Xiang-Dang Du,Henrike Krüger,Andrea T. Feßler,Shizhen Ma,Yao Zhu,Congming Wu,Jianzhong Shen,Yang Wang +10 more
TL;DR: In this paper, the authors identified seven mobile oxazolidinone resistance genes, including cfr, cfr(B), cfr (C), Cfr(C), cf(C, cfc(D), Cf(E), optrA, and poxtA, on plasmids.
Journal ArticleDOI
Oxazolidinones: mechanisms of resistance and mobile genetic elements involved.
TL;DR: This review provides an overview on the diversity of the mobile genetic elements carrying oxazolidinone resistance genes in Gram-positive and Gram-negative bacteria.
Journal ArticleDOI
Multidrug-Resistant Methicillin-Resistant Coagulase-Negative Staphylococci in Healthy Poultry Slaughtered for Human Consumption
Vanessa Silva,Manuela Caniça,Eugénia Ferreira,Madalena Vieira-Pinto,C. Saraiva,José Eduardo Pereira,José Luis Capelo,Gilberto Igrejas,Patrícia Poeta +8 more
TL;DR: There was a frequent occurrence of multidrug resistance in MRCoNS isolated from healthy poultry in Portugal, and most isolates showed resistance to erythromycin, clindamycin, penicillin, tetracycline, ciprofloxacin and fusidic acid and harbored the ermB, ermC, mphC, tetK, tetL, tetM and tetO genes.
Journal ArticleDOI
Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus from Hunters and Hunting Dogs
Vanessa Silva,Manuela Caniça,Vera Manageiro,Madalena Vieira-Pinto,José Eduardo Pereira,Luís Maltez,Patrícia Poeta,Gilberto Igrejas +7 more
TL;DR: A moderate colonization of S. aureus in hunting dogs and their owners was detected in this study and a few dog- to-dog and dog-to-human possible transmissions were identified.
Journal ArticleDOI
Effect of efflux pump inhibitors on antimicrobial activity of florfenicol against resistant strains of porcine actinobacillus pleuropneumoniae and pasteurella multocida
TL;DR: In this paper, the authors investigated whether combinational use of efflux pump inhibitors (EPIs) could improve florfenicol (FF) antimicrobial activity and found that five EPIs including Carbonyl cyanide chloro...
References
More filters
Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
RNAmmer: consistent and rapid annotation of ribosomal RNA genes
Karin Lagesen,Peter Fischer Hallin,Einar Andreas Rødland,Hans Henrik Stærfeldt,Torbjørn Rognes,David W. Ussery +5 more
TL;DR: Results from running RNAmmer on a large set of genomes indicate that the location of rRNAs can be predicted with a very high level of accuracy.
Journal ArticleDOI
Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation.
Sergey Koren,Brian P. Walenz,Konstantin Berlin,Jason R. Miller,Nicholas H. Bergman,Adam M. Phillippy +5 more
TL;DR: Canu, a successor of Celera Assembler that is specifically designed for noisy single-molecule sequences, is presented, demonstrating that Canu can reliably assemble complete microbial genomes and near-complete eukaryotic chromosomes using either Pacific Biosciences or Oxford Nanopore technologies.
Journal ArticleDOI
Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: A microbiological and molecular biological study
Yiyun Liu,Yang Wang,Timothy R. Walsh,Ling-Xian Yi,Rong Zhang,James Spencer,Yohei Doi,Guo-Bao Tian,Baolei Dong,Xianhui Huang,Lin-Feng Yu,Danxia Gu,Hongwei Ren,Xiaojie Chen,Luchao Lv,Dandan He,Hongwei Zhou,Zi-sen Liang,Jian-Hua Liu,Jianzhong Shen +19 more
TL;DR: The emergence of MCR-1 heralds the breach of the last group of antibiotics, polymyxins, by plasmid-mediated resistance, in Enterobacteriaceae and emphasise the urgent need for coordinated global action in the fight against pan-drug-resistant Gram-negative bacteria.
Journal ArticleDOI
tRNAscan-SE On-line: integrating search and context for analysis of transfer RNA genes
Todd M. Lowe,Patricia P. Chan +1 more
TL;DR: This update of the tRNAscan-SE On-line resource ties together improvements in tRNA classification with greatly enhanced biological context via dynamically generated links between web server search results, the most relevant genes in the GtRNAdb and interactive, rich genome context provided by UCSC genome browsers.