Community structures of fecal bacteria in cattle from different animal feeding operations.
Orin C. Shanks,Catherine A. Kelty,S. L. Archibeque,Michael B. Jenkins,Ryan J. Newton,Sandra L. McLellan,Susan M. Huse,Mitchell L. Sogin +7 more
TLDR
Network analysis demonstrated that annotated sequences clustered by management practice and fecal starch concentration, suggesting that the structures of bovine fecal bacterial communities can be dramatically different in different animal feeding operations, even at the phylum and family taxonomic levels.Abstract:
The fecal microbiome of cattle plays a critical role not only in animal health and productivity but also in food safety, pathogen shedding, and the performance of fecal pollution detection methods. Unfortunately, most published molecular surveys fail to provide adequate detail about variability in the community structures of fecal bacteria within and across cattle populations. Using massively parallel pyrosequencing of a hypervariable region of the rRNA coding region, we profiled the fecal microbial communities of cattle from six different feeding operations where cattle were subjected to consistent management practices for a minimum of 90 days. We obtained a total of 633,877 high-quality sequences from the fecal samples of 30 adult beef cattle (5 individuals per operation). Sequence-based clustering and taxonomic analyses indicate less variability within a population than between populations. Overall, bacterial community composition correlated significantly with fecal starch concentrations, largely reflected in changes in the Bacteroidetes, Proteobacteria, and Firmicutes populations. In addition, network analysis demonstrated that annotated sequences clustered by management practice and fecal starch concentration, suggesting that the structures of bovine fecal bacterial communities can be dramatically different in different animal feeding operations, even at the phylum and family taxonomic levels, and that the feeding operation is a more important determinant of the cattle microbiome than is the geographic location of the feedlot.read more
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Journal ArticleDOI
The 16S rDNA high-throughput sequencing correlation analysis of milk and gut microbial communities in mastitis Holstein cows
Xiaolu Hou,Xiaona Gao,Pei Liu,Xiao Min Guo,Guoliang Hu,Qingqing Li,Cheng Huang,Guyue Li,Weile Fang,Wanrui Mai,Cong Wu,Zheng Xu,Minghui Liu +12 more
TL;DR: In this paper , the authors extracted microbial DNA from healthy and mastitis cows and performed high-throughput sequencing using the Illumina NovaSeq sequencing platform to understand the changes in the milk and gut microbiota of dairy cows with mastitis, and to further explore the relationship between mastitis and the microbiota.
Posted ContentDOI
Short- and long-term effects of continuous compost amendment on soil microbiome community
Judith Kraut-Cohen,Avihai Zolti,Nativ Rotbart,Asher Bar-Tal,Yael Laor,Shlomit Medina,Raneen Shawahna,Ibrahim Saadi,Michael Raviv,Stefan J. Green,Uri Yermiyahu,Dror Minz +11 more
TL;DR: In this paper , the authors examined the immediate and long-term temporal dynamics of agricultural soil microbial communities following repeated compost applications at two field sites: Newe Ya'ar (NY, Mediterranean climate) and Gilat (G, semi-arid climate).
Journal ArticleDOI
我国不同地区奶牛乳房炎病原菌群落多样性特征 Diversity of Pathogenic Microbimes Associated with Bovine Mastitis across Different Regions of China
TL;DR: It is shown that diversity of pathogenic microbiomes in mastitis-affected cow milk from dif-ferent regions is a prerequisite for clarifying etiological factors and preventing and controlling bovine mastitis effectively.
Book ChapterDOI
Microbiome-Based Sustainable Agriculture Targeting Plant Protection
TL;DR: The root-associated microbiota deploys their repertoire of secondary metabolites to antagonize pathogens even before they get to the plant, acting as the true first line of defense while also priming systemic plant defense as discussed by the authors .
Journal ArticleDOI
House feeding system improves the estrus rate in yaks (Bos grunniens) by increasing specific fecal microbiota and myo-inositol content in serum
Ya Jun Zhu,Xin Xian Li,Lousang zhaxi,Suolang Zhaxi,Suolang,Ciyang,Guangming Sun,Cidan YangJi,Basang Wangdui +8 more
TL;DR: The HF system promoted the estrus rate and changed the composition of yak fecal microbiota and serum metabolites, which could lead to a novel therapeutic strategy for G yaks due to their lowEstrus rate.
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