Journal ArticleDOI
CRISPR/Cas12a collateral cleavage activity for simple and rapid detection of protein/small molecule interaction
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TLDR
In this paper, a CRISPR/Cas12a collateral cleavage activity was used to detect protein/small molecule interactions by modifying a single-stranded activator DNA modified with a specific small molecule.About:
This article is published in Biosensors and Bioelectronics.The article was published on 2021-08-25. It has received 19 citations till now. The article focuses on the topics: Trans-activating crRNA & CRISPR.read more
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Electrochemiluminescence covalent organic framework coupling with CRISPR/Cas12a-mediated biosensor for pesticide residue detection.
TL;DR: In this paper , an electrochemiluminescence (ECL) covalent organic framework (COF) based-biosensor was developed for trace pesticide detection coupling with CRISPR/Cas12a-mediated signal accumulation strategy.
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CRISPR-Cas12a-Based Aptasensor for On-Site and Highly Sensitive Detection of Microcystin-LR in Freshwater.
TL;DR: A CRISPR-Cas12a-based aptasensor platform (named as MC-LR-Casor) for on-site and sensitive detection of microcystin-LR (MC-LR) showed excellent selectivity and good recovery rates, demonstrating their good applicability for real water sample analysis.
Journal ArticleDOI
Powerful CRISPR-Based Biosensing Techniques and Their Integration With Microfluidic Platforms
TL;DR: This review provides an overview of recent advances in the development of CRISPR-based biosensing techniques and their perfect combination with microfluidic platforms and their various applications in healthcare, animal husbandry, agriculture, and forestry.
Journal ArticleDOI
CRISPR/Cas14 provides a promising platform in facile and versatile aptasensing with improved sensitivity.
TL;DR: In this paper , a fluorometric biosensor named HARRY (highly sensitive aptamer-regulated Cas14 R-loop for bioanalysis) was developed, which can detect ATP, Cd2+, histamine, aflatoxin B1, and thrombin with detection limits at the low-nanomolar level, which shows improvement compared with Cas12a-based aptasensors in sensitivity and versatility.
Journal ArticleDOI
PAM-independent ultra-specific activation of CRISPR-Cas12a via sticky-end dsDNA
Wei Zhang,Yaoqin Mu,Kejun Dong,Lei Zhang,Bei Yan,Hao Hu,Yan Liao,Rong-Wei Zhao,Wan Shu,Zhengxin Ye,Yaping Lu,Chong Wan,Qiangqiang Sun,Longjie Li,Hongbo Wang,Xian Lim Xiao +15 more
TL;DR: Wang et al. as discussed by the authors discovered a new targeting substrate for LbaCas12a (Lachnospiraceae bacterium Cas12a), namely double-stranded DNA (dsDNA) with a sticky-end region (PAM−SE+ dsDNA).
References
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Journal ArticleDOI
Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics.
Shao En Ong,Blagoy Blagoev,Irina Kratchmarova,Dan B. Kristensen,Hanno Steen,Akhilesh Pandey,Matthias Mann +6 more
TL;DR: SILAC is a simple, inexpensive, and accurate procedure that can be used as a quantitative proteomic approach in any cell culture system and is applied to the relative quantitation of changes in protein expression during the process of muscle cell differentiation.
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Development and applications of CRISPR-Cas9 for genome engineering.
TL;DR: In this paper, the authors describe the development and applications of Cas9 for a variety of research or translational applications while highlighting challenges as well as future directions, and highlight challenges and future directions.
Journal ArticleDOI
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
TL;DR: A review of achievements made possible by site-specific nuclease technologies and applications of these reagents for genetic analysis and manipulation, including the therapeutic potential of ZFNs and TALENs, and future prospects for the field are discussed.
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Towards a proteome-scale map of the human protein–protein interaction network
Jean François Rual,Kavitha Venkatesan,Tong Hao,Tomoko Hirozane-Kishikawa,Amélie Dricot,Ning Li,Gabriel F. Berriz,Francis D. Gibbons,Matija Dreze,Nono Ayivi-Guedehoussou,Niels Klitgord,Christophe Simon,Mike Boxem,Stuart Milstein,Jennifer Rosenberg,Debra S. Goldberg,Lan V. Zhang,Sharyl L. Wong,Giovanni Franklin,Siming Li,Joanna S. Albala,Joanna S. Albala,Janghoo Lim,Carlene Fraughton,Estelle Llamosas,Sebiha Cevik,Camille Bex,Philippe Lamesch,Robert S. Sikorski,Jean Vandenhaute,Huda Y. Zoghbi,Alex Smolyar,Stephanie Bosak,Reynaldo Sequerra,Lynn Doucette-Stamm,Michael E. Cusick,David E. Hill,Frederick P. Roth,Marc Vidal +38 more
TL;DR: An initial version of a proteome-scale map of human binary protein–protein interactions is described, which increases by ∼70% the set of available binary interactions within the tested space and reveals more than 300 new connections to over 100 disease-associated proteins.
Journal ArticleDOI
A human protein-protein interaction network : a resource for annotating the proteome
Ulrich Stelzl,Uwe Worm,Maciej Lalowski,Christian Haenig,Felix H. Brembeck,Heike Goehler,Martin Stroedicke,Martina Zenkner,Anke Schoenherr,Susanne Koeppen,Jan Timm,Sascha Mintzlaff,Claudia Abraham,Nicole Bock,Silvia Kietzmann,Astrid Goedde,Engin Toksöz,Anja Droege,Sylvia Krobitsch,Bernhard Korn,Walter Birchmeier,Hans Lehrach,Erich E. Wanker +22 more
TL;DR: A large, highly connected network of interacting pairs of human proteins was identified, characterizing ANP32A and CRMP1 as modulators of Wnt signaling and two novel Axin-1 interactions were validated experimentally.