Defining Molecular Cornerstones during Fibroblast to iPS Cell Reprogramming in Mouse
Reads0
Chats0
TLDR
Using doxycycline-inducible vectors, it is shown that exogenous factors are required for about 10 days, after which cells enter a self-sustaining pluripotent state and markers are identified that define cell populations prior to and during this transition period.About:
This article is published in Cell Stem Cell.The article was published on 2008-03-06 and is currently open access. It has received 908 citations till now. The article focuses on the topics: Reprogramming & Induced pluripotent stem cell.read more
Citations
More filters
Journal ArticleDOI
Disease-Specific Induced Pluripotent Stem Cells
In-Hyun Park,Natasha Arora,Hongguang Huo,Nimet Maherali,Tim Ahfeldt,Tim Ahfeldt,Akiko Shimamura,M. William Lensch,M. William Lensch,Chad A. Cowan,Konrad Hochedlinger,George Q. Daley +11 more
TL;DR: The generation of induced pluripotent stem cells from patients with a variety of genetic diseases with either Mendelian or complex inheritance are described, offering an unprecedented opportunity to recapitulate both normal and pathologic human tissue formation in vitro, thereby enabling disease investigation and drug development.
Journal ArticleDOI
Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors
TL;DR: The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of i PS cells in regenerative medicine.
Journal ArticleDOI
piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
Knut Woltjen,Iacovos P. Michael,Iacovos P. Michael,Paria Mohseni,Paria Mohseni,Ridham Desai,Ridham Desai,Maria Mileikovsky,Riikka H. Hämäläinen,Rebecca Cowling,Wei Wang,Pentao Liu,Marina Gertsenstein,Keisuke Kaji,Hoon Ki Sung,Andras Nagy,Andras Nagy +16 more
TL;DR: It is shown that the individual PB insertions can be removed from established iPS cell lines, providing an invaluable tool for discovery, and the traceless removal of reprogramming factors joined with viral 2A sequences delivered by a single transposon from murine iPS lines is demonstrated.
Journal ArticleDOI
Induced pluripotent stem cells generated without viral integration
Matthias Stadtfeld,Masaki Nagaya,Masaki Nagaya,Jochen Utikal,Gordon C. Weir,Gordon C. Weir,Konrad Hochedlinger +6 more
TL;DR: This work generated mouse induced pluripotent stem cells from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc, providing strong evidence that insertional mutagenesis is not required for in vitro reprogramming.
Journal ArticleDOI
Stem Cells, the Molecular Circuitry of Pluripotency and Nuclear Reprogramming
Rudolf Jaenisch,Richard A. Young +1 more
TL;DR: In this article, the authors review strategies to reprogram somatic cells to a pluripotent embryonic state and discuss their understanding of the molecular mechanisms of reprogramming based on recent insights into the regulatory circuitry of the PLSTM.
References
More filters
Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development.
TL;DR: It is demonstrated that two recently identified DNA methyltransferases, DnMT3a and Dnmt3b, are essential for de novo methylation and for mouse development and play important roles in normal development and disease.
Journal ArticleDOI
Viable offspring derived from fetal and adult mammalian cells
TL;DR: The birth of lambs from differentiated fetal and adult cells confirms that differentiation of that cell did not involve the irreversible modification of genetic material required for development to term and reinforces previous speculation that by inducing donor cells to become quiescent it will be possible to obtain normal development from a wide variety of differentiated cells.