Dissemination prevention of antibiotic resistant and facultative pathogenic bacteria by ultrafiltration and ozone treatment at an urban wastewater treatment plant.
TLDR
Conventional wastewater treatment is not sufficient for the removal of hygienically relevant bacteria and achieves only limited reductions, so metagenome analyses demonstrated the accumulation of facultative pathogenic bacteria, antibiotic resistance genes, virulence factor genes, and metabolic gene targets in the back flush retentates of the membranes, which opens further questions about retentate fluid material handling at urban wastewater treatment plants.Abstract:
Conventional wastewater treatment is not sufficient for the removal of hygienically relevant bacteria and achieves only limited reductions. This study focuses on the reduction efficiencies of two semi-industrial ultrafiltration units operating at a large scale municipal wastewater treatment plant. In total, 7 clinically relevant antibiotic resistance genes, together with 3 taxonomic gene markers targeting specific facultative pathogenic bacteria were analysed via qPCR analyses before and after advanced treatment. In parallel with membrane technologies, an ozone treatment (1 g ozone/g DOC) was performed for comparison of the different reduction efficiencies. Both ultrafiltration units showed increased reduction efficiencies for facultative pathogenic bacteria and antibiotic resistance genes of up to 6 log units, resulting mostly in a strong reduction of the bacterial targets. In comparison, the ozone treatment showed some reduction efficiency, but was less effective compared with ultrafiltration due to low ozone dosages frequently used for micro-pollutant removal at municipal wastewater treatment plants. Additionally, metagenome analyses demonstrated the accumulation of facultative pathogenic bacteria, antibiotic resistance genes, virulence factor genes, and metabolic gene targets in the back flush retentate of the membranes, which opens further questions about retentate fluid material handling at urban wastewater treatment plants.read more
Citations
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Ultrafiltration membranes for wastewater and water process engineering: A comprehensive statistical review over the past decade
TL;DR: In this article, a review of research interest and current research trends concerning UF membrane applications and processes within the past decade (2009-2018) is presented, highlighting the present-day significance of UF membranes along with their prospective opportunities for attaining sustainability in water industries and materializing the efforts of future researchers.
Journal ArticleDOI
Evaluation of antibiotic resistance dissemination by wastewater treatment plant effluents with different catchment areas in Germany.
TL;DR: Increased correlation of FPB with clinically relevant ARGs of the category of rarely occurring resistance genes was found in WWTP effluents being influenced by hospital wastewaters, finding no significant difference between ARG categories and WWTP catchment areas.
Journal ArticleDOI
Problems of conventional disinfection and new sterilization methods for antibiotic resistance control
TL;DR: The effects and drawbacks of conventional disinfection methods, such as chlorine (Cl), ultraviolet (UV) and ozone on antibiotic resistance control, and the research progress and shortcomings of new sterilization methods in antibiotic resistance are analyzed.
Journal ArticleDOI
ESKAPE Bacteria and Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolated from Wastewater and Process Water from German Poultry Slaughterhouses
Mykhailo Savin,Gabriele Bierbaum,Jens A. Hammerl,Céline Heinemann,Marijo Parcina,Esther Sib,Alexander Voigt,Judith Kreyenschmidt +7 more
TL;DR: W wastewater effluents from the investigated poultry slaughterhouses exhibited clinically relevant bacteria, including E. coli, methicillin-resistant S. aureus, K. pneumoniae, and species of the ACB and Enterobacter cloacae complexes that contribute to the dissemination of clinically relevant resistances in the environment.
Journal ArticleDOI
The investigation of antibiotic residues, antibiotic resistance genes and antibiotic-resistant organisms in a drinking water reservoir system in Germany.
Alexander Voigt,P. Ciorba,Manuel Döhla,Martin Exner,C. Felder,Franziska Lenz-Plet,Esther Sib,D. Skutlarek,Ricarda Maria Schmithausen,H.A. Faerber +9 more
TL;DR: In this specific catchment area, which lacked clinical, hospitals, and intensive livestock farming, no significant bacterial or analyzed chemical (antibiotic residues) influences of the sewage treatment plants were observed in the river downstream of the drinking water reservoir during the study.
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