Open AccessBook
Drosophila: A Laboratory Handbook
Reads0
Chats0
TLDR
This manual covers three approaches to the field: analysis of neural development, recording and imaging activities in the nervous system, and analysis of behavior.Abstract:
Drosophila Neurobiology-Bing Zhang 2010 Based on Cold Spring Harbor Laboratory's long-running course, Drosophila Neurobiology: A Laboratory Manual offers detailed protocols and background material for researchers interested in using Drosophila as an experimental model for investigating the nervous system. This manual covers three approaches to the field: analysis of neural development, recording and imaging activities in the nervous system, and analysis of behavior. Techniques described include molecular, genetic, electrophysiological, imaging, behavioral and developmental methods.read more
Citations
More filters
Journal ArticleDOI
Analysis of genetic mosaics in developing and adult Drosophila tissues
Tian Xu,Gerald M. Rubin +1 more
TL;DR: This work has constructed a series of strains to facilitate the generation and analysis of clones of genetically distinct cells in developing and adult tissues of Drosophila, providing an unprecedented opportunity to perform systematic genetic screens for mutations affecting many biological processes.
Journal ArticleDOI
The Genome Sequence of the Malaria Mosquito Anopheles gambiae
Robert A. Holt,G. Mani Subramanian,Aaron L. Halpern,Granger G. Sutton,Rosane Charlab,Deborah R. Nusskern,Patrick Wincker,Andrew G. Clark,José M. C. Ribeiro,Ron Wides,Steven L. Salzberg,Brendan J. Loftus,Mark Yandell,William H. Majoros,William H. Majoros,Douglas B. Rusch,Zhongwu Lai,Cheryl L. Kraft,Josep F. Abril,Véronique Anthouard,Peter Arensburger,Peter W. Atkinson,Holly Baden,Véronique de Berardinis,Danita Baldwin,Vladimir Benes,Jim Biedler,Claudia Blass,Randall Bolanos,Didier Boscus,Mary Barnstead,Shuang Cai,Kabir Chatuverdi,George K. Christophides,Mathew A. Chrystal,Michele Clamp,Anibal Cravchik,Val Curwen,Ali N Dana,Arthur L. Delcher,Ian M. Dew,Cheryl A. Evans,Michael Flanigan,Anne Grundschober-Freimoser,Lisa Friedli,Zhiping Gu,Ping Guan,Roderic Guigó,Maureen E. Hillenmeyer,Susanne L. Hladun,James R. Hogan,Young S. Hong,Jeffrey Hoover,Olivier Jaillon,Zhaoxi Ke,Zhaoxi Ke,Chinnappa D. Kodira,Kokoza Eb,Anastasios C. Koutsos,Ivica Letunic,Alex Levitsky,Yong Liang,Jhy-Jhu Lin,Jhy-Jhu Lin,Neil F. Lobo,John Lopez,Joel A. Malek,Tina C. McIntosh,Stephan Meister,Jason R. Miller,Clark M. Mobarry,Emmanuel Mongin,Sean D. Murphy,David A. O'Brochta,Cynthia Pfannkoch,Rong Qi,Megan A. Regier,Karin A. Remington,Hongguang Shao,Maria V. Sharakhova,Cynthia Sitter,Jyoti Shetty,Thomas J. Smith,Renee Strong,Jingtao Sun,Dana Thomasova,Lucas Q. Ton,Pantelis Topalis,Zhijian Tu,Maria F. Unger,Brian P. Walenz,Aihui Wang,Jian Wang,Mei Wang,X. Wang,Kerry J. Woodford,Jennifer R. Wortman,Jennifer R. Wortman,Martin Wu,Alison Yao,Evgeny M. Zdobnov,Hongyu Zhang,Qi Zhao,Shaying Zhao,Shiaoping C. Zhu,Igor F. Zhimulev,Mario Coluzzi,Alessandra della Torre,Charles Roth,Christos Louis,Francis Kalush,Richard J. Mural,Eugene W. Myers,Mark Raymond Adams,Hamilton O. Smith,Samuel Broder,Malcolm J. Gardner,Claire M. Fraser,Ewan Birney,Peer Bork,Paul T. Brey,J. Craig Venter,J. Craig Venter,Jean Weissenbach,Fotis C. Kafatos,Frank H. Collins,Stephen L. Hoffman +126 more
TL;DR: Analysis of the PEST strain of A. gambiae revealed strong evidence for about 14,000 protein-encoding transcripts, and prominent expansions in specific families of proteins likely involved in cell adhesion and immunity were noted.
Journal ArticleDOI
Cytosine methylation and the ecology of intragenomic parasites
TL;DR: It has become increasingly difficult to hold that reversible promoter methylation is commonly involved in developmental gene control; instead, suppression of parasitic sequence elements appears to be the primary function of cytosine methylation, with crucial secondary roles in allele-specific gene expression as seen in X inactivation and genomic imprinting.
Journal ArticleDOI
Rates of Spontaneous Mutation
TL;DR: It is now possible to specify some of the evolutionary forces that shape these diverse mutation rates in broad groups of organisms.
Journal ArticleDOI
The effect of deleterious mutations on neutral molecular variation.
TL;DR: Observed reductions in molecular variation in low recombination genomic regions of sufficiently large size, for instance in the centromere-proximal regions of Drosophila autosomes or in highly selfing plant populations, may be partly due to background selection against deleterious mutations.
Related Papers (5)
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Andrea H. Brand,Norbert Perrimon +1 more
The genome sequence of Drosophila melanogaster
Mark Raymond Adams,Susan E. Celniker,Robert A. Holt,Cheryl A. Evans,Jeannine D. Gocayne,Peter Amanatides,Steve Scherer,Peter W. Li,Roger A. Hoskins,R. Galle,Reed A. George,Suzanna E. Lewis,Stephen Richards,Michael Ashburner,Scott Henderson,Granger G. Sutton,Jennifer R. Wortman,Mark Yandell,Qing Zhang,Lin Chen,Rhonda C. Brandon,Yu-Hui Rogers,R. Blazej,Mark Champe,Barret D. Pfeiffer,Kenneth H. Wan,Colleen Doyle,E. G. Baxter,Gregg Helt,Catherine R. Nelson,G. L. Gabor Miklos,Josep F. Abril,A. Agbayani,Huijin An,C. Andrews-Pfannkoch,Danita Baldwin,Richard M. Ballew,Anand Basu,James Baxendale,Leyla Bayraktaroglu,Ellen M. Beasley,Karen Beeson,Panayiotis V. Benos,Benjamin P. Berman,D. Bhandari,Slava Bolshakov,Dana Borkova,Michael R. Botchan,John Bouck,Peter Brokstein,Philippe Brottier,Kenneth C. Burtis,Dana A. Busam,Heather Butler,Edouard Cadieu,I. Chandra,J. Michael Cherry,Simon Cawley,Carl Dahlke,Lionel Davenport,P. Davies,B. de Pablos,Arthur L. Delcher,Zuoming Deng,A. Deslattes Mays,Ian M. Dew,Susanne Dietz,Kristina Dodson,Lisa Doup,Michael Downes,Shannon Dugan-Rocha,B. C. Dunkov,Patrick J. Dunn,K. J. Durbin,Carlos Evangelista,Concepcion Ferraz,Steven Ferriera,Wolfgang Fleischmann,Carl Fosler,Andrei Gabrielian,Neha Garg,William M. Gelbart,Kenneth Glasser,A. Glodek,Fangcheng Gong,J. Harley Gorrell,Zhiping Gu,Ping Guan,Michael Harris,Nomi L. Harris,Damon A. Harvey,Thomas J. Heiman,Judith Hernandez,Jarrett Houck,Damon Hostin,K. Houston,Timothy Howland,Ming-Hui Wei,Chinyere Ibegwam,M. Jalali,Francis Kalush,Gary H. Karpen,Zhaoxi Ke,James A. Kennison,K. A. Ketchum,B. E. Kimmel,Chinnappa D. Kodira,Cheryl L. Kraft,Saul A. Kravitz,David Kulp,Zhongwu Lai,Paul Lasko,Yiding Lei,Alexander Levitsky,Jun Li,Zhenya Li,Yunye Liang,Xiaoying Lin,Xiangjun Liu,B. Mattei,Tina C. McIntosh,Michael P. McLeod,D. McPherson,Gennady V. Merkulov,Natalia Milshina,Clark M. Mobarry,J. Morris,A. Moshrefi,Stephen M. Mount,Mee Moy,Brian Murphy,Lee Murphy,Donna M. Muzny,David L. Nelson,David R. Nelson,Keith Nelson,K. Nixon,Deborah R. Nusskern,Joanne Pacleb,Michael J. Palazzolo,G. S. Pittman,Sue Pan,J. Pollard,Vinita Puri,Martin G. Reese,Knut Reinert,Karin A. Remington,Robert D. C. Saunders,Robert D. C. Saunders,F. Scheeler,H. Shen,B. Christopher Shue,Inga Siden-Kiamos,Michael Simpson,Marian P. Skupski,Thomas J. Smith,Eugene G. Spier,Allan C. Spradling,Mark Stapleton,Renee Strong,E. Sun,Robert Svirskas,C. Tector,Russell Turner,Eli Venter,Aihui Wang,Xianyuan Wang,Zhen Yuan Wang,David A. Wassarman,George M. Weinstock,Jean Weissenbach,Sherita Williams,Trevor Woodage,Kim C. Worley,D. Wu,Shih-Hung Yang,Q. Alison Yao,Jane Ye,R. F. Yeh,Jayshree Zaveri,Ming Zhan,Gefei Zhang,Qi Zhao,Liansheng Zheng,Xiangqun Zheng,Fei Zhong,Wenyan Zhong,X. Zhou,Shiaoping C. Zhu,Xiancan Zhu,Hamilton O. Smith,Richard A. Gibbs,Eugene W. Myers,Gerald M. Rubin,J. Craig Venter +194 more