Drosophila protamine-like Mst35Ba and Mst35Bb are required for proper sperm nuclear morphology but are dispensable for male fertility.
Samantha Tirmarche,Shuhei Kimura,Laure Sapey-Triomphe,William J. Sullivan,Frédéric Landmann,Benjamin Loppin +5 more
TLDR
Drosophila males homozygous for a genomic deletion covering several genes including the protamine-like genes Mst35Ba/b are surprisingly fertile, and this work precisely deleted the Mst 35B locus by homologous recombination, and it is confirmed the dispensability of Mst34B for fertility.Abstract:
During spermiogenesis, histones are massively replaced with protamines. A previous report showed that Drosophila males homozygous for a genomic deletion covering several genes including the protamine-like genes Mst35Ba/b are surprisingly fertile. Here, we have precisely deleted the Mst35B locus by homologous recombination, and we confirm the dispensability of Mst35Ba/b for fertility.read more
Citations
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The Wolbachia cytoplasmic incompatibility enzyme CidB targets nuclear import and protamine-histone exchange factors
TL;DR: CidB targets nuclear-protein import and protamine-histone exchange and that CidA rescues embryos by restricting CidB access to its targets and this is proposed to be a rescue mechanism for embryos.
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The dynamics and regulation of chromatin remodeling during spermiogenesis.
TL;DR: This review synthesize and summarize the current knowledge on the progress of chromatin remodeling during spermiogenesis, and straighten out the chronological order of chromatis remodeling and illustrate the possible regulation mechanisms of each step.
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The intimate genetics of Drosophila fertilization
TL;DR: The current knowledge of fertilization in Drosophila melanogaster is reviewed, with a special emphasis on the genes involved in the complex transformation of the fertilizing sperm nucleus into a replicated set of paternal chromosomes.
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Unlocking sperm chromatin at fertilization requires a dedicated egg thioredoxin in Drosophila.
TL;DR: It is demonstrated that the Drosophila maternal thioredoxin Deadhead (DHD) is specifically required to unlock sperm chromatin at fertilization and is then rapidly degraded after fertilization.
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The Drosophila chromosomal protein Mst77F is processed to generate an essential component of mature sperm chromatin.
Shuhei Kimura,Benjamin Loppin +1 more
TL;DR: It is demonstrated that Mst77F is incorporated in spermatid chromatin as a precursor protein, which is subsequently processed through the proteolysis of its N-terminus and leaves the cysteine residues in the mature protein intact, suggesting that they participate in the formation of disulfide cross-links.
References
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Protamines and spermatogenesis in Drosophila and Homo sapiens : A comparative analysis.
TL;DR: In this article, the authors examined spermatogenesis and the final packaging of DNA into the sperm head in the insect Drosophila melanogaster and compare it to sperminogenesis in Homo sapiens.
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Interacting genes identify interacting proteins involved in microtubule function in Drosophila.
Margaret T. Fuller,Cathy L. Regan,Larry L. Green,Barbara Robertson,Renate Deuring,Thomas S. Hays +5 more
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Drosophila I-R hybrid dysgenesis is associated with catastrophic meiosis and abnormal zygote formation.
TL;DR: It is shown that meiotic divisions in SF oocytes are catastrophic and systematically fail to produce a functional female pronucleus at fertilization, suggesting that I-element activity might perturb the functional organization of meiotic chromosomes without triggering an early DNA damage response.
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Control of mouse hils1 gene expression during spermatogenesis: identification of regulatory element by transgenic mouse.
TL;DR: Transgenic mice are analyzed to demonstrate that 318-base pair 5′-proximal region corresponding to the first 70-bp proximal TATA-less promoter, and 248 bp of5′-untranslated region is sufficient to confer testis- and spermatid-specific transcription as well as posttranscriptional control of the mouse hils1 gene in vivo.
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Protamine-like proteins in 12 sequenced species of Drosophila.
TL;DR: This study represents the first large-scale, single-genus dataset for protamine-like proteins and provides the basis for a fine-grained analysis of their evolution.