Effective Inhibition of HBV Replication in Vivo by Anti-HBx Short Hairpin RNAs
Sergio Carmona,Abdullah Ely,Carol Crowther,Naazneen Moolla,Felix H. Salazar,Patricia L. Marion,Nicolas Ferry,Marc S. Weinberg,Patrick Arbuthnot +8 more
TLDR
Observations indicate that U6 shRNA 5 and U6ShRNAs 6 are promising candidates for therapy of chronic HBV infection.About:
This article is published in Molecular Therapy.The article was published on 2006-02-01 and is currently open access. It has received 113 citations till now. The article focuses on the topics: Small hairpin RNA & Hepatitis B virus.read more
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Hydrodynamic gene delivery: its principles and applications.
Takeshi Suda,Dexi Liu +1 more
TL;DR: This review provides an overview of the theory and practice of hydrodynamic gene delivery so as to aid researchers for the use of this method in their pre-clinical and translational gene therapy studies.
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miR-122, a paradigm for the role of microRNAs in the liver
Muriel Girard,Muriel Girard,Muriel Girard,Emmanuel Jacquemin,Arnold Munnich,Arnold Munnich,Arnold Munnich,Stanislas Lyonnet,Stanislas Lyonnet,Stanislas Lyonnet,Alexandra Henrion-Caude,Alexandra Henrion-Caude,Alexandra Henrion-Caude +12 more
TL;DR: The biology of the most abundant miRNA in human liver, miR-122, is reviewed, and the diversity of its roles in the liver is considered, and some possible therapeutic opportunities for exploiting miRNAs in the different settings of liver diseases are considered.
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RNAi for Treating Hepatitis B Viral Infection
TL;DR: Several strategies for improving the anti-HBV therapeutic efficacy of siRNAs, while avoiding their off-target effects and immunostimulation are discussed.
Journal ArticleDOI
Controlling HBV replication in vivo by intravenous administration of triggered PEGylated siRNA-nanoparticles.
Sergio Carmona,Michael R Jorgensen,Soumia Kolli,Carol Crowther,Felix H. Salazar,Patricia L. Marion,Masato Fujino,Yukikazu Natori,Maya Thanou,Patrick Arbuthnot,Andrew D. Miller +10 more
TL;DR: A new hepatotropic nontoxic lipid-based vector system that is used to deliver chemically unmodified small interfering RNA (siRNA) sequences to the liver and repeated systemic administration of triggered PEGylated siRNA-nanoparticles to HBV transgenic mice results in the suppression of markers of HBV replication by up to 3-fold relative to controls over a 28 day period.
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Expressed anti-HBV primary microRNA shuttles inhibit viral replication efficiently in vitro and in vivo
TL;DR: An approach that allows the use of a Pol II promoter to improve transcription regulation of expressed RNAi effecters is reported, and Employing Pol II-expressed pri-miR mimics may be useful in the treatment of HBV infection, and potentially also for generic application in RNAi-based therapy.
References
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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
TL;DR: 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
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A System for Stable Expression of Short Interfering RNAs in Mammalian Cells
TL;DR: It is shown that siRNA expression mediated by this vector causes efficient and specific down-regulation of gene expression, resulting in functional inactivation of the targeted genes.
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RNA interference is mediated by 21- and 22-nucleotide RNAs
TL;DR: In this article, the authors demonstrate that 21 and 22-nt RNA fragments are the sequence-specific mediators of RNA interference in a Drosophila in vitro system, and provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the siRNA-protein complex.
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A simplified system for generating recombinant adenoviruses
TL;DR: In this paper, a recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eucaryotic cells.
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An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells
TL;DR: It is shown that ‘loss-of-function’ phenotypes can be created in cultured Drosophila cells by transfection with specific double-stranded RNAs, which coincides with a marked reduction in the level of cognate cellular messenger RNAs.