Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presence of a divergent cytochrome c oxidase.
Azadeh Seidi,Linden Muellner-Wong,Esther Rajendran,Erick T. Tjhin,Laura F. Dagley,Laura F. Dagley,Vincent Yt Aw,Pierre Faou,Andrew I. Webb,Andrew I. Webb,Christopher J. Tonkin,Christopher J. Tonkin,Giel G. van Dooren +12 more
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TLDR
This study identifies approximately 400 mitochondrial proteins, many of which lack homologs in the animals that these parasites infect, and most of which are important for parasite growth, and demonstrates that one such protein, termed TgApiCox25, is an important component of the parasite cytochrome c oxidase (COX) complex.Abstract:
The mitochondrion of apicomplexan parasites is critical for parasite survival, although the full complement of proteins that localize to this organelle has not been defined Here we undertake two independent approaches to elucidate the mitochondrial proteome of the apicomplexan Toxoplasma gondii We identify approximately 400 mitochondrial proteins, many of which lack homologs in the animals that these parasites infect, and most of which are important for parasite growth We demonstrate that one such protein, termed TgApiCox25, is an important component of the parasite cytochrome c oxidase (COX) complex We identify numerous other apicomplexan-specific components of COX, and conclude that apicomplexan COX, and apicomplexan mitochondria more generally, differ substantially in their protein composition from the hosts they infect Our study highlights the diversity that exists in mitochondrial proteomes across the eukaryotic domain of life, and provides a foundation for defining unique aspects of mitochondrial biology in an important phylum of parasitesread more
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Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging
Hyun-Woo Rhee,Peng Zou,Namrata D. Udeshi,Jeffrey D. Martell,Vamsi K. Mootha,Vamsi K. Mootha,Steven A. Carr,Alice Y. Ting,Alice Y. Ting +8 more
TL;DR: In this article, a peroxidase-based method was used to identify 495 proteins within the human mitochondrial matrix, including 31 proteins not previously linked to mitochondria, and the labeling was exceptionally specific and distinguished between inner membrane proteins facing the matrix versus the intermembrane space.
A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes
Saima M. Sidik,Diego Huet,My Hang Huynh,Prathapan Thiru,Jeroen P. J. Saeij,Vern B. Carruthers,Sebastian Lourido,Suresh M. Ganesan,Armiyaw S. Nasamu,Jacquin C. Niles,Timothy C. Wang +10 more
TL;DR: The first genome-wide genetic screen of an apicomplexan parasite is presented, revealing essential functions during infection of human cells and providing broad-based functional information on T. gondii genes that will facilitate future approaches to expand the horizon of antiparasitic interventions.
Journal ArticleDOI
The draft nuclear genome sequence and predicted mitochondrial proteome of Andalucia godoyi , a protist with the most gene-rich and bacteria-like mitochondrial genome
Michael W. Gray,Gertraud Burger,Romain Derelle,Vladimír Klimeš,Michelle M. Leger,Michelle M. Leger,Matt Sarrasin,Čestmír Vlček,Andrew J. Roger,Marek Eliáš,B. Franz Lang +10 more
TL;DR: The draft nuclear genome sequence for the jakobid Andalucia godoyi is assembled and a comprehensive in silico approach is used to infer the nucleus-encoded portion of the mitochondrial proteome of this protist, identifying 864 candidate mitochondrial proteins.
Book ChapterDOI
ToxoDB: Functional Genomics Resource for Toxoplasma and Related Organisms.
Omar S. Harb,David S. Roos +1 more
TL;DR: ToxoDB is a free online resource that provides access to genomic and functional genomic data that enables scientists to build in silico experiments and develop testable hypothesis.
Journal ArticleDOI
Composition and stage dynamics of mitochondrial complexes in Plasmodium falciparum.
Felix Evers,Alfredo Cabrera-Orefice,Dei M. Elurbe,Mariska Kea-te Lindert,Sylwia D. Boltryk,Sylwia D. Boltryk,Till S. Voss,Till S. Voss,Martijn A. Huynen,Ulrich Brandt,Ulrich Brandt,Taco W. A. Kooij +11 more
TL;DR: This article applied complexome profiling to map the inventory of protein complexes across the pathogenic asexual blood stages and the transmissible gametocyte stages of Plasmodium falciparum and found that respiratory chain complex components and linked metabolic pathways are up to 40 times more prevalent in gametocytes, while glycolytic enzymes are substantially reduced.
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