Journal ArticleDOI
Epigenetic events in mammalian germ-cell development: reprogramming and beyond
Hiroyuki Sasaki,Yasuhisa Matsui +1 more
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TLDR
It is shown that epigenetic modifiers have key roles in germ-cell development itself and contributes to the gene-expression programme that is required for germ- cell development, regulation of meiosis and genomic integrity.Abstract:
The epigenetic profile of germ cells, which is defined by modifications of DNA and chromatin, changes dynamically during their development. Many of the changes are associated with the acquisition of the capacity to support post-fertilization development. Our knowledge of this aspect has greatly increased- for example, insights into how the re-establishment of parental imprints is regulated. In addition, an emerging theme from recent studies is that epigenetic modifiers have key roles in germ-cell development itself--for example, epigenetics contributes to the gene-expression programme that is required for germ-cell development, regulation of meiosis and genomic integrity. Understanding epigenetic regulation in germ cells has implications for reproductive engineering technologies and human health.read more
Citations
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Characterization of a novel fluorescent reporter of genomic imprinting in the mouse
TL;DR: The first imprinted fluorescent transgene is described, a green fluorescent protein (GFP) gene inserted in the distal MMU7 imprinted domain between the imprinting centers 1 and 2 (IC1 and IC2) regulated regions, which exhibits imprinted expression only from the maternal allele, and is silenced and DNA methylated on the paternal allele in post-implantation embryos.
Metylacja DNA a otyłość prosta DNA methylation in obesity
Beata Kieć-Wilk,Anna Polus +1 more
TL;DR: In this review, the known changes in the profile of DNA methylation as a factor affecting obesity and its complications are described.
Journal ArticleDOI
Sequences in the H19 ICR that are transcribed as small RNA in oocytes are dispensable for methylation imprinting in YAC transgenic mice
Takuya Takahashi,Hitomi Matsuzaki,Shin ichi Tomizawa,Shin ichi Tomizawa,Eiichi Okamura,Tomoko Ichiyanagi,Akiyoshi Fukamizu,Hiroyuki Sasaki,Keiji Tanimoto +8 more
TL;DR: It is shown that sequences in the H19 ICR that correspond to the small RNA sequences are dispensable for methylation imprinting in YAC TgM, demonstrating that differential methylation could be established after fertilization.
Journal ArticleDOI
Intrauterine exposure of mice to arsenite induces abnormal and transgenerational glycometabolism.
TL;DR: In this article , the effects of arsenite exposure on the metabolic phenotypes and the levels of proteins and genes related to glucose metabolism of dams and their offspring were investigated in mice.
Book ChapterDOI
The DNA Methylation Machinery
TL;DR: This chapter describes the current understanding of the mechanisms and molecules involved in the addition, and maintenance, of methyl groups to cytosine bases and the mechanism by which these 5-mC modifications are read resulting in changes in gene expression.
References
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Journal ArticleDOI
A Bivalent Chromatin Structure Marks Key Developmental Genes in Embryonic Stem Cells
Bradley E. Bernstein,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Xiaohui Xie,Michael Kamal,Dana J. Huebert,James Cuff,Ben Fry,Alexander Meissner,Marius Wernig,Kathrin Plath,Rudolf Jaenisch,Alexandre Wagschal,Robert Feil,Stuart L. Schreiber,Stuart L. Schreiber,Eric S. Lander,Eric S. Lander +17 more
TL;DR: It is proposed that bivalent domains silence developmental genes in ES cells while keeping them poised for activation, highlighting the importance of DNA sequence in defining the initial epigenetic landscape and suggesting a novel chromatin-based mechanism for maintaining pluripotency.
Journal ArticleDOI
Generation of germline-competent induced pluripotent stem cells
TL;DR: iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.
Journal ArticleDOI
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
Marius Wernig,Alexander Meissner,Ruth K. Foreman,Tobias Brambrink,Manching Ku,Konrad Hochedlinger,Konrad Hochedlinger,Bradley E. Bernstein,Bradley E. Bernstein,Rudolf Jaenisch +9 more
TL;DR: The results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells.
Journal ArticleDOI
Epigenetic Transgenerational Actions of Endocrine Disruptors and Male Fertility
TL;DR: The ability of an environmental factor to reprogram the germ line and to promote a transgenerational disease state has significant implications for evolutionary biology and disease etiology.
Journal ArticleDOI
Environmental epigenomics and disease susceptibility.
TL;DR: An increasing body of evidence from animal studies supports the role of environmental epigenetics in disease susceptibility and recent studies have demonstrated for the first time that heritable environmentally induced epigenetic modifications underlie reversible transgenerational alterations in phenotype.
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Ryan Lister,Mattia Pelizzola,Robert H. Dowen,R. David Hawkins,Gary C. Hon,Julian Tonti-Filippini,Joseph R. Nery,Leonard Lee,Zhen Ye,Que Minh Ngo,Lee Edsall,Jessica Antosiewicz-Bourget,Jessica Antosiewicz-Bourget,Ron Stewart,Ron Stewart,Victor Ruotti,Victor Ruotti,A. Harvey Millar,James A. Thomson,Bing Ren,Bing Ren,Joseph R. Ecker +21 more