Journal ArticleDOI
Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity
Jianhua Li,Kuancheng Liu,Yang Liu,Yan Xu,Fei Zhang,Huijuan Yang,Jiangxia Liu,Tingting Pan,Jieliang Chen,Min Wu,Xiaohui Zhou,Zhenghong Yuan +11 more
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TLDR
An antiviral mechanism of IFN-α activity that involves the induction and intercellular transfer of antiviral molecules via exosomes is proposed and contributed to the antiviral response of IFn-α to mouse hepatitis virus A59 and adenovirus in mice.Abstract:
The cell-to-cell transmission of viral resistance is a potential mechanism for amplifying the interferon-induced antiviral response. In this study, we report that interferon-α (IFN-α) induced the transfer of resistance to hepatitis B virus (HBV) from nonpermissive liver nonparenchymal cells (LNPCs) to permissive hepatocytes via exosomes. Exosomes from IFN-α-treated LNPCs were rich in molecules with antiviral activity. Moreover, exosomes from LNPCs were internalized by hepatocytes, which mediated the intercellular transfer of antiviral molecules. Finally, we found that exosomes also contributed to the antiviral response of IFN-α to mouse hepatitis virus A59 and adenovirus in mice. Thus, we propose an antiviral mechanism of IFN-α activity that involves the induction and intercellular transfer of antiviral molecules via exosomes.read more
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Expression of Serum Exosomal miRNA 122 and Lipoprotein Levels in Dogs Naturally Infected by Leishmania infantum: A Preliminary Study.
Antonio Di Loria,Vincenzo Dattilo,Domenico Santoro,Jacopo Guccione,Adriana De Luca,Paolo Ciaramella,Marinella Pirozzi,Enrico Iaccino +7 more
TL;DR: The decreased expression of exosomal miR122 represents an additional effect of Leishmania infection in dogs as in people, and results suggest that hepatic dysfunctions induced by the parasite interfere with lipoprotein status.
Journal ArticleDOI
Targeting endothelial exosomes for the prevention of cardiovascular disease.
Guanghong Jia,James R. Sowers +1 more
TL;DR: This review highlights recent studies involving endothelial exosomes, gives a brief overview of exosome biogenesis and release, isolation and identification, and provides a contemporary understanding of the endothelialExosome pathophysiology and potential therapeutic strategies.
Journal ArticleDOI
Attack, parry and riposte: molecular fencing between the innate immune system and human herpesviruses.
TL;DR: Current knowledge on IFN antagonistic strategies of the eight human herpesviruses are summarized and a molecular in-depth understanding of (herpes-) viral IFN antagonists might allow the design of novel antiviral drugs which reconstitute IFN responses by blocking the antagonistic function and thereby help the host to help himself.
Journal ArticleDOI
Exosomes Released from Rabies Virus-Infected Cells May be Involved in the Infection Process.
Jingyu Wang,Fan Wu,C Liu,Wenwen Dai,Yawei Teng,Weiheng Su,Wei Kong,Feng Gao,Linjun Cai,Ali Hou,Chunlai Jiang +10 more
TL;DR: The results showed that rabies virus infection increased the release of exosomes, and the inhibitors reduced the levels of extracellular and intracellular viral RNA, indicating that exosome may participate in the viral infection process.
Journal ArticleDOI
Hypoxic colorectal cancer-derived extracellular vesicles deliver microRNA-361-3p to facilitate cell proliferation by targeting TRAF3 via the noncanonical NF-κB pathways.
Jie Li,Peng Yang,Fangyu Chen,Yuqian Tan,Changzhi Huang,Hengyang Shen,Chaofan Peng,Yifei Feng,Yueming Sun +8 more
TL;DR: In this paper, high-throughput sequencing was employed to detect aberrantly expressed microRNAs (miRNAs) in hypoxic extracellular vesicles (EVs) and quantitative real-time PCR was used to confirm and screen preliminarily candidate miRNAs.
References
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Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TL;DR: It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).
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Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
TL;DR: This unit describes different approaches for exosome purification from various sources, and discusses methods to evaluate the purity and homogeneity of the purified exosomes preparations.
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Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers
Johan Skog,T. Wurdinger,van Rijn S,Dimphna H. Meijer,Gainche L,Miguel Sena-Esteves,William T. Curry,Bob S. Carter,Anna M. Krichevsky,Xandra O. Breakefield +9 more
TL;DR: Tumour-derived microvesicles may provide diagnostic information and aid in therapeutic decisions for cancer patients through a blood test by incorporating an mRNA for a reporter protein into them, and it is demonstrated that messages delivered by microvesicle are translated by recipient cells.
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Membrane vesicles as conveyors of immune responses
TL;DR: The role of membrane vesicles, in particular exosomes, in the communication between immune cells, and between tumour and immune cells is focused on.
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Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes
Katarina Trajkovic,Chieh Hsu,Salvatore Chiantia,Lawrence Rajendran,Dirk Wenzel,Felix T. Wieland,Petra Schwille,Britta Brügger,Mikael Simons +8 more
TL;DR: It is found that cargo is segregated into distinct subdomains on the endosomal membrane and that the transfer of exosome-associated domains into the lumen of theendosome did not depend on the function of the ESCRT (endosomal sorting complex required for transport) machinery, but required the sphingolipid ceramide.