Extended-Spectrum β-Lactamase–Producing Escherichia coli From Retail Chicken Meat and Humans: Comparison of Strains, Plasmids, Resistance Genes, and Virulence Factors
Jan Kluytmans,I. T. M. A. Overdevest,Ina Willemsen,Marjolein F. Q. Kluytmans-van den Bergh,Kim van der Zwaluw,Max Heck,Martine C. Rijnsburger,Christina M. J. E. Vandenbroucke-Grauls,Paul H. M. Savelkoul,Brian D. Johnston,David M. Gordon,James R. Johnson +11 more
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Significant genetic similarities among ESBL-EC isolates from chicken meat and humans according to mobile resistance elements, virulence genes, and genomic backbone are found and raises serious food safety questions regarding the abundant presence of ES BL-EC in chicken meat.Abstract:
BACKGROUND: The worldwide prevalence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is increasing rapidly both in hospitals and in the community. A connection between ESBL-producing bacteria in food animals, retail meat, and humans has been suggested. We previously reported on the genetic composition of a collection of ESBL-producing Escherichia coli (ESBL-EC) from chicken meat and humans from a restricted geographic area. Now, we have extended the analysis with plasmid replicons, virulence factors, and highly discriminatory genomic profiling methods. METHODS: One hundred forty-five ESBL-EC isolates from retail chicken meat, human rectal carriers, and blood cultures were analyzed using multilocus sequence typing, phylotyping, ESBL genes, plasmid replicons, virulence genes, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE). RESULTS: Three source groups overlapped substantially when their genetic composition was compared. A combined analysis using all variables yielded the highest resolution (Wilks lambda [Lambda]: 0.08). Still, a prediction model based on the combined data classified 40% of the human isolates as chicken meat isolates. AFLP and PFGE showed that the isolates from humans and chicken meat could not be segregated and identified 1 perfect match between humans and chicken meat. CONCLUSIONS: We found significant genetic similarities among ESBL-EC isolates from chicken meat and humans according to mobile resistance elements, virulence genes, and genomic backbone. Therefore, chicken meat is a likely contributor to the recent emergence of ESBL-EC in human infections in the study region. This raises serious food safety questions regarding the abundant presence of ESBL-EC in chicken meat.read more
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References
More filters
Journal ArticleDOI
Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.
Fred C. Tenover,Robert D. Arbeit,Richard V. Goering,P. A. Mickelsen,Barbara E Murray,D. H. Persing,B. Swaminathan +6 more
TL;DR: This research presents a novel, scalable and scalable approach that allows for real-time assessment of the severity of the infection and its impact on patients’ health.
Journal ArticleDOI
Rapid and simple determination of the Escherichia coli phylogenetic group.
TL;DR: In this paper, a simple and rapid phylogenetic grouping technique based on triplex PCR was proposed, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.
Journal ArticleDOI
Identification of plasmids by PCR-based replicon typing
Alessandra Carattoli,Alessia Bertini,Laura Villa,Vincenzo Falbo,Katie L. Hopkins,E. John Threlfall +5 more
TL;DR: Results indicated that the inc/rep PCR method demonstrates high specificity and sensitivity in detecting replicons on reference plasmids and also revealed the presence of recurrent and common plasmid in epidemiologically unrelated Salmonella isolates of different serotypes.
Journal ArticleDOI
Sex and virulence in Escherichia coli: an evolutionary perspective
Thierry Wirth,Daniel Falush,Ruiting Lan,Frances M. Colles,Patience Mensa,Lothar H. Wieler,Helge Karch,Peter R. Reeves,Martin C. J. Maiden,Howard Ochman,Mark Achtman +10 more
TL;DR: The evolution of virulence is linked to bacterial sex because rates of evolution have accelerated in pathogenic lineages, culminating in highly virulent organisms whose genomic contents are altered frequently by increased rates of homologous recombination.
Journal ArticleDOI
Resistance Plasmid Families in Enterobacteriaceae
TL;DR: Bacteria carry extrachromosomal, self-replicating genetic elements called plasmids, which do not carry genes essential for the growth of host cells under nonstressed conditions.