Extraproteasomal Rpn10 Restricts Access of the Polyubiquitin-Binding Protein Dsk2 to Proteasome
Yulia Matiuhin,Donald S. Kirkpatrick,Inbal Ziv,Woong Kim,Arun Dakshinamurthy,Oded Kleifeld,Steven P. Gygi,Noa Reis,Michael H. Glickman +8 more
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TLDR
By restricting Dsk2 access to the proteasome, extraproteasomal Rpn10 was essential for alleviating the cellular stress associated with DSk2, highlighting the importance of polyubiquitin shuttles such as RPN10 and Dsk1 in controlling the ubiquitin landscape.About:
This article is published in Molecular Cell.The article was published on 2008-11-07 and is currently open access. It has received 95 citations till now. The article focuses on the topics: Ubiquitin-conjugating enzyme & Polyubiquitin binding.read more
Citations
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Recognition and Processing of Ubiquitin-Protein Conjugates by the Proteasome
TL;DR: The proteasome contains deubiquitinating enzymes (DUBs) that can remove ubiquitin before substrate degradation initiates, thus allowing some substrates to dissociate from the proteasomes and escape degradation.
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Ubiquitin-binding domains — from structures to functions
TL;DR: New structure-based insights provide strategies for controlling cellular processes by targeting ubiquitin–UBD interfaces with implications for drug design and cell reprograming.
Journal ArticleDOI
Regulation of proteasome activity in health and disease.
Marion Schmidt,Daniel Finley +1 more
TL;DR: The contributions of the proteasome to human pathology, mechanisms that regulate the proteolytic capacity of the Proteasome are described, and strategies to modulate proteasomes function as a therapeutic approach to ameliorate diseases associated with altered UPS function are discussed.
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The Ubiquitin–Proteasome System of Saccharomyces cerevisiae
TL;DR: The diverse effects of ubiquitylation reflect the assembly of structurally distinct ubiquitin chains on target proteins.
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Gene Overexpression: Uses, Mechanisms, and Interpretation
TL;DR: The history of overexpression, the mechanisms that are responsible for overexploration phenotypes, tests that begin to distinguish between those mechanisms, the varied ways in which overexposure is used, the methods and reagents available in several organisms, and the relevance of overexpression to human disease are described.
References
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Recognition of the polyubiquitin proteolytic signal.
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A proteomics approach to understanding protein ubiquitination
Junmin Peng,Junmin Peng,Daniel Schwartz,Joshua E. Elias,Carson C. Thoreen,Dongmei Cheng,Gerald T. Marsischky,Jeroen Roelofs,Daniel Finley,Steven P. Gygi +9 more
TL;DR: A proteomics approach to enrich, recover, and identify ubiquitin conjugates from Saccharomyces cerevisiae lysate provides a general tool for the large-scale analysis and characterization of protein ubiquitination.