Gene knock-out chain reaction enables high disruption efficiency of HPV18 E6/E7 genes in cervical cancer cells
Ping Zhou,Xun Tian,marreneczq +2 more
TLDR
In this paper , a gene knockout chain reaction (GKCR) method was developed for continually generating mutagenic disruptions and used this method to disrupt the HPV18 E6 and E7 genes.About:
This article is published in Molecular Therapy - Oncolytics.The article was published on 2022-03-01 and is currently open access. It has received 9 citations till now. The article focuses on the topics: CRISPR & Gene.read more
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Mechanistic Role of HPV-Associated Early Proteins in Cervical Cancer: Molecular Pathways and Targeted Therapeutic Strategies.
Rahul Bhattacharjee,Sabya Sachi Das,Smrutihara Biswal,Arijit Nath,Debangshi Das,Asmita Basu,Sumira Malik,Lamha Kumar,Sulagna Kar,Sandeep Singh,Vijay Upadhye,Danish Iqbal,Suliman Almojam,Shubhadeep Roychoudhury,Shreesh Ojha,Janne Ruokolainen,Niraj K. Jha,Kavindra Kumar Kesari +17 more
TL;DR: A systematic search has been carried out for articles published in PubMed database by using combinations of different keywords with Boolean operators (AND, OR, NOT) including cervical cancer, HPV, E proteins, and signaling as discussed by the authors .
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Advanced Nanomedicine for High-Risk HPV-Driven Head and Neck Cancer
Qiang-hua Xu,Ye Chen,Yuan Jin,Zhiyu Wang,Haoru Dong,Andreas M. Kaufmann,Andreas E. Albers,Xu Qian +7 more
TL;DR: A review of the recent advancements observed in preclinical studies and clinical trials on HR-HPV-driven head and neck squamous cell carcinoma (HNSCC) focusing on nanotechnology related methods is presented in this article .
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Therapeutic Application of Genome Editing Technologies in Viral Diseases
Tae-Hyeong Kim,Seong-Wook Lee +1 more
TL;DR: The current status of the development of viral therapeutic agents using genome editing technology is looked at and how this technology can be used as a new treatment approach for viral diseases is discussed.
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A review on CRISPR/Cas: a versatile tool for cancer screening, diagnosis, and clinic treatment
Xianguang Yang,Baohong Zhang +1 more
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Genome Editing Approaches with CRISPR/Cas9 for Cancer Treatment: Critical Appraisal of Preclinical and Clinical Utility, Challenges, and Future Research
Sergiu Chira,Andreea Nutu,Ecaterina Isacescu,Cecilia Bica,Laura Pop,Cristina Ciocan,Ioana Berindan-Neagoe +6 more
TL;DR: Preclinical studies and clinical trials that use CRISPR-based technology for therapeutic purposes of cancer and a summary of the presented studies adds a more focused view of the therapeutic value CRISpr/Cas9 holds and the associated shortcomings.
References
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
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CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
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Papillomaviruses and cancer: from basic studies to clinical application
TL;DR: Links between human papillomaviruses (HPVs) and cervical cancer were first suspected almost 30 years ago and DNA of specific HPV types has since been found in almost all cervical cancer biopsies.
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RNA-programmed genome editing in human cells
TL;DR: It is shown here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA breaks at a site complementary to the guide RNA sequence in genomic DNA.
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Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
TL;DR: It is shown that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33% in human cells.