Generation of Induced Pluripotent Stem Cells from Human Cord Blood
Alexandra Haase,Ruth Olmer,Kristin Schwanke,Stephanie Wunderlich,Sylvia Merkert,Christian Hess,Robert Zweigerdt,Ina Gruh,Johann Meyer,Stefan Wagner,Lars S. Maier,Dong Wook Han,Silke Glage,Konstantin Miller,Philipp Fischer,Hans R. Schöler,Ulrich Martin +16 more
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TLDR
The generation of human iPSCs from cord blood (CB) as a juvenescent cell source is reported, showing characteristics typical of embryonic stem cells and can be differentiated into derivatives of all three germ layers, including functional cardiomyocytes.About:
This article is published in Cell Stem Cell.The article was published on 2009-10-02 and is currently open access. It has received 489 citations till now. The article focuses on the topics: Stem cell & Induced pluripotent stem cell.read more
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Induced pluripotency: history, mechanisms, and applications
TL;DR: This review summarizes the progress that has been made in the iPSC field over the last 4 years, with an emphasis on understanding the mechanisms of cellular reprogramming and its potential applications in cell therapy.
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Senescence impairs successful reprogramming to pluripotent stem cells
Ana Banito,Sheikh Tamir Rashid,Juan Carlos Acosta,Si De Li,Carlos Filipe Pereira,Imbisaat Geti,Sandra Pinho,José C. R. Silva,Véronique Azuara,Martin J. Walsh,Ludovic Vallier,Jesús Gil +11 more
TL;DR: Crucially, ablation of different senescence effectors improves the efficiency of reprogramming, suggesting novel strategies for maximizing the generation of iPS cells.
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Generation of human induced pluripotent stem cells from urine samples
Ting Zhou,Christina Benda,Sarah Dunzinger,Yinghua Huang,Jenny Cy Ho,Jiayin Yang,Yu Wang,Ya Zhang,Qiang Zhuang,Yanhua Li,Xichen Bao,Hung-Fat Tse,Hung-Fat Tse,Hung-Fat Tse,Johannes Grillari,Regina Grillari-Voglauer,Duanqing Pei,Miguel A. Esteban,Miguel A. Esteban +18 more
TL;DR: Urinary iPSCs (UiPSCs) also show excellent differentiation potential, and thus represent a good choice for producing pluripotent cells from normal individuals or patients with genetic diseases, including those affecting the kidney.
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Harnessing the potential of induced pluripotent stem cells for regenerative medicine
TL;DR: It is believed that a better understanding of the molecular basis of pluripotency, cellular reprogramming and lineage-specific differentiation of iPSCs is necessary for progress in regenerative medicine.
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Induced pluripotent stem cells — opportunities for disease modelling and drug discovery
TL;DR: How iPSCs are being exploited to illuminate disease pathophysiology, identify novel drug targets and enhance the probability of clinical success of new drugs is discussed.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
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In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector
Luigi Naldini,Ulrike Blömer,Philippe Gallay,Daniel S. Ory,Richard C. Mulligan,Fred H. Gage,Inder M. Verma,Didier Trono +7 more
TL;DR: The ability of HIV-based viral vectors to deliver genes in vivo into nondividing cells could increase the applicability of retroviral vectors in human gene therapy.
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Induction of Pluripotent Stem Cells From Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: This work generated induced pluripotent stem cells capable of germline transmission from murine somatic cells by transd, and demonstrated the ability of these cells to reprogram into patient-specific and disease-specific stem cells.