Harnessing the potential of induced pluripotent stem cells for regenerative medicine
TLDR
It is believed that a better understanding of the molecular basis of pluripotency, cellular reprogramming and lineage-specific differentiation of iPSCs is necessary for progress in regenerative medicine.Abstract:
The discovery of methods to convert somatic cells into induced pluripotent stem cells (iPSCs) through expression of a small combination of transcription factors has raised the possibility of producing custom-tailored cells for the study and treatment of numerous diseases. Indeed, iPSCs have already been derived from patients suffering from a large variety of disorders. Here we review recent progress that has been made in establishing iPSC-based disease models, discuss associated technical and biological challenges, and highlight possible solutions to overcome these barriers. We believe that a better understanding of the molecular basis of pluripotency, cellular reprogramming and lineage-specific differentiation of iPSCs is necessary for progress in regenerative medicine.read more
Citations
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Pluripotent Stem Cells Induced from Mouse Somatic Cells by Small-Molecule Compounds
Pingping Hou,Yanqin Li,Xu Zhang,Chun Liu,Jingyang Guan,Honggang Li,Ting Zhao,Junqing Ye,Weifeng Yang,Kang Liu,Jian Ge,Jun Xu,Qiang Zhang,Yang Zhao,Hongkui Deng +14 more
TL;DR: It is shown that pluripotent stem cells can be generated from mouse somatic cells at a frequency up to 0.2% using a combination of seven small-molecule compounds, which resemble embryonic stem cells in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission.
Journal ArticleDOI
A Molecular Roadmap of Reprogramming Somatic Cells into iPS Cells
Jose M. Polo,Endre Anderssen,Endre Anderssen,Ryan M. Walsh,Benjamin A. Schwarz,Christian M. Nefzger,Sue Mei Lim,Marti Borkent,Marti Borkent,Effie Apostolou,Sara Alaei,Jennifer Cloutier,Ori Bar-Nur,Sihem Cheloufi,Matthias Stadtfeld,Maria E. Figueroa,Daisy A. Robinton,Sridaran Natesan,Ari Melnick,Jinfang Zhu,Sridhar Ramaswamy,Sridhar Ramaswamy,Konrad Hochedlinger,Konrad Hochedlinger +23 more
TL;DR: This integrative analysis allowed us to identify genes that act as roadblocks during reprogramming and surface markers that further enrich for cells prone to forming iPSCs, and offer new mechanistic insights into the nature and sequence of molecular events inherent to cellular reprograming.
Journal ArticleDOI
Origin and differentiation of microglia.
TL;DR: The history of microglial cells is reviewed and the latest advances in the understanding of their origin, differentiation, and homeostasis are discussed, which provides new insights into their roles in health and disease.
Journal ArticleDOI
Next-generation proteomics: towards an integrative view of proteome dynamics
TL;DR: MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis, and to highlight recent applications.
Journal ArticleDOI
Paths to stemness: building the ultimate antitumour T cell
TL;DR: It is discussed how using stem cell-like T cells can overcome the limitations of current adoptive T cell therapies, including inefficient T cell engraftment, persistence and ability to mediate prolonged immune attack.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
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Generation of germline-competent induced pluripotent stem cells
TL;DR: iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.
Journal ArticleDOI
Induction of Pluripotent Stem Cells From Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: This work generated induced pluripotent stem cells capable of germline transmission from murine somatic cells by transd, and demonstrated the ability of these cells to reprogram into patient-specific and disease-specific stem cells.