Genes Contributing to Porphyromonas gingivalis Fitness in Abscess and Epithelial Cell Colonization Environments
Daniel P. Miller,Justin A Hutcherson,Yan Wang,Zuzanna Nowakowska,Jan Potempa,Jan Potempa,Deborah R. Yoder-Himes,David A. Scott,Marvin Whiteley,Richard J. Lamont +9 more
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TLDR
This study verifies the importance of several well-characterized putative virulence factors of P. gingivalis and identifies novel fitness determinants of the organism.Abstract:
Porphyromonas gingivalis is an important cause of serious periodontal diseases, and is emerging as a pathogen in several systemic conditions including some forms of cancer. Initial colonization by P. gingivalis involves interaction with gingival epithelial cells, and the organism can also access host tissues and spread haematogenously. To better understand the mechanisms underlying these properties, we utilized a highly saturated transposon insertion library of P. gingivalis, and assessed the fitness of mutants during epithelial cell colonization and survival in a murine abscess model by high-throughput sequencing (Tn-Seq). Transposon insertions in many genes previously suspected as contributing to virulence showed significant fitness defects in both screening assays. In addition, a number of genes not previously associated with P. gingivalis virulence were identified as important for fitness. We further examined fitness defects of four such genes by generating defined mutations. Genes encoding a carbamoyl phosphate synthetase, a replication-associated recombination protein, a nitrosative stress responsive HcpR transcription regulator, and RNase Z, a zinc phosphodiesterase, showed a fitness phenotype in epithelial cell colonization and in a competitive abscess infection. This study verifies the importance of several well-characterized putative virulence factors of P. gingivalis and identifies novel fitness determinants of the organism.read more
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The Role of Exopolysaccharides in Oral Biofilms.
TL;DR: This review presents the current research on exopolysaccharides of oral microbes regarding their biosynthesis, regulation, contributions to biofilm formation and stability of the matrix, and immune evasion, as well as a potential nutrient source for species within a biofilm.
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The potential role of P.gingivalis in gastrointestinal cancer: a mini review
TL;DR: The relationship among P.gingivalis, periodontal disease, and digestive tract tumors must be clarified through a multi-center, prospective, large-scale study.
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Identification of Potential Candidate Genes of Oral Cancer in Response to Chronic Infection With Porphyromonas gingivalis Using Bioinformatical Analyses.
TL;DR: Microarray data obtained from the chronic infection model of immortalized oral epithelial cells that were persistently exposed to P. gingivalis were analyzed to identify hub genes, major biological processes, upstream regulators and genes potentially involved in tumor initiation and progression that might facilitate the prevention and treatment of oral squamous cell carcinoma.
Journal ArticleDOI
Variability in Genomic and Virulent Properties of Porphyromonas gingivalis Strains Isolated From Healthy and Severe Chronic Periodontitis Individuals.
Katterinne N. Mendez,Anilei Hoare,Cristopher Soto,Isaac Bugueño,Marcela Olivera,Claudio Meneses,José M. Pérez-Donoso,Eduardo Castro-Nallar,Denisse Bravo +8 more
TL;DR: Genetic differences between CP3 and H3 are in agreement with both lower biofilm formation ability and less intracellular invasion to oral epithelial cells exhibited by H3, compared with the virulent strain CP3.
Journal ArticleDOI
Porphyromonas gingivalis Tyrosine Phosphatase Php1 Promotes Community Development and Pathogenicity.
Young-Jung Jung,Daniel P. Miller,John D. Perpich,Zackary R. Fitzsimonds,Daonan Shen,Jun Ohshima,Richard J. Lamont +6 more
TL;DR: It is shown that Porphyromonas gingivalis, a Gram-negative periodontal pathogen, expresses a PHP protein, Php1, with divalent metal ion-dependent tyrosine phosphatase activity, a unique configuration in Gram-negatives which may allow P. gedivalis to maintain phosphorylation/dephosphorylation homeostasis in multispecies communities.
References
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Stephen R. Coats,Jace W. Jones,Christopher T. Do,Pamela H. Braham,Brian W. Bainbridge,Thao T. To,David R. Goodlett,Robert K. Ernst,Richard P. Darveau +8 more
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LPXTG Protein InlJ, a Newly Identified Internalin Involved in Listeria monocytogenes Virulence
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