Journal ArticleDOI
Genetic diversity in Sargasso Sea bacterioplankton.
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TLDR
The phylogenetically analysed clone libraries of eubacterial 16S ribosomal RNA genes amplified from natural populations of Sargasso Sea picoplankton indicate the presence of a novel microbial group, the SAR 11 cluster, which appears to be a significant component of this oligotrophic bacterioplankton community.Abstract:
BACTERIOPLANKTON are recognized as important agents of biogeochemical change in marine ecosystems, yet relatively little is known about the species that make up these communities. Uncertainties about the genetic structure and diversity of natural bacterioplankton populations stem from the traditional difficulties associated with microbial cultivation techniques. Discrepancies between direct counts and plate counts are typically several orders of magnitude, raising doubts as to whether cultivated marine bacteria are actually representative of dominant planktonic species1–3. We have phylogenetically analysed clone libraries of eubacterial 16S ribosomal RNA genes amplified from natural populations of Sargasso Sea picoplankton by the polymerase chain reaction4. The analysis indicates the presence of a novel microbial group, the SAR 11 cluster, which appears to be a significant component of this oligotrophic bacterioplankton community. A second cluster of lineages related to the oxygenic phototrophs—cyanobacteria, prochlorophytes and chloroplasts—was also observed. However, none of the genes matched the small subunit rRNA sequences of cultivated marine cyanobacteria from similar habitats. The diversity of 16S rRNA genes observed within the clusters suggests that these bacterioplankton may be consortia of independent lineages sharing surprisingly distant common ancestors.read more
Citations
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Diversity of endocytic bacteria in the dinoflagellate Noctiluca scintillans
TL;DR: The results indicate the occurrence of 1 dominant group of endocytic bacteria and some other groups of less dominance in Noctiluca scintillans, indicating the development of a specific bacterial population within N. scintsillans.
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Investigation of 0.2 μm filterable bacteria from the Western Mediterranean Sea using a molecular approach: dominance of potential starvation forms
TL;DR: DGGE patterns indicate that most of the fragments representing 0.2 µm filterable bacteria were rather starvation forms of marine bacteria than ultramicrobacteria, which is in contrast to the current knowledge concerning the status of 0.1 µmfilterable bacteria.
Journal ArticleDOI
Population structure of algicidal marine bacteria targeting the red tide forming alga Heterosigma akashiwo (Raphidophyceae), determined by restriction fragment length polymorphism analysis of the bacterial 16S ribosomal RNA genes
TL;DR: Partial sequences, almost 500 bp of nucleotides, and RFLP patterns of 16s rDNA from some HAKB strains revealed that the H AKB of 2B, 2C and 2D ribotypes are closely related to the 'I-proteobacteria group.
Journal ArticleDOI
Ecogenomics of the SAR11 clade
Jose M. Haro-Moreno,Francisco Rodriguez-Valera,Francisco Rodriguez-Valera,Riccardo Rosselli,Francisco Martinez-Hernandez,Juan J. Roda-Garcia,Monica Lluesma Gomez,Oscar Fornas,Manuel Martinez-Garcia,Mario López-Pérez +9 more
TL;DR: A glimpse of the enormous genomic diversity within this SAR11 clade is provided at a finer resolution than the currently defined clades, and it is revealed that the flexible genome was essential to shape genomospecies distribution.
Journal ArticleDOI
The microbial diversity in picoplankton enrichment cultures: a molecular screening of marine isolates
TL;DR: The data show the enormous diversity of cultivated bacteria from picoplankton enrichment cultures of one North Sea water sample, which is only a small fraction of the total picoplANKton community.
References
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Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
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TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Journal ArticleDOI
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
TL;DR: In this paper, complementary oligodeoxyribonucleotide (oligo) primers and the polymerase chain reaction are used to generate two DNA fragments having overlapping ends, and these fragments are combined in a subsequent 'fusion' reaction in which the overlapping ends anneal, allowing the 3' overlap of each strand to serve as a primer for the three' extension of the complementary strand.
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