Journal ArticleDOI
Genetic diversity in Sargasso Sea bacterioplankton.
TLDR
The phylogenetically analysed clone libraries of eubacterial 16S ribosomal RNA genes amplified from natural populations of Sargasso Sea picoplankton indicate the presence of a novel microbial group, the SAR 11 cluster, which appears to be a significant component of this oligotrophic bacterioplankton community.Abstract:
BACTERIOPLANKTON are recognized as important agents of biogeochemical change in marine ecosystems, yet relatively little is known about the species that make up these communities. Uncertainties about the genetic structure and diversity of natural bacterioplankton populations stem from the traditional difficulties associated with microbial cultivation techniques. Discrepancies between direct counts and plate counts are typically several orders of magnitude, raising doubts as to whether cultivated marine bacteria are actually representative of dominant planktonic species1–3. We have phylogenetically analysed clone libraries of eubacterial 16S ribosomal RNA genes amplified from natural populations of Sargasso Sea picoplankton by the polymerase chain reaction4. The analysis indicates the presence of a novel microbial group, the SAR 11 cluster, which appears to be a significant component of this oligotrophic bacterioplankton community. A second cluster of lineages related to the oxygenic phototrophs—cyanobacteria, prochlorophytes and chloroplasts—was also observed. However, none of the genes matched the small subunit rRNA sequences of cultivated marine cyanobacteria from similar habitats. The diversity of 16S rRNA genes observed within the clusters suggests that these bacterioplankton may be consortia of independent lineages sharing surprisingly distant common ancestors.read more
Citations
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Journal ArticleDOI
Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies.
Rebecca J. Case,Yan Boucher,Ingela Dahllöf,Carola Holmström,W. Ford Doolittle,Staffan Kjelleberg +5 more
TL;DR: The single-copy gene rpoB provided comparable phylogenetic resolution to that of the 16S rRNA gene at all taxonomic levels, except between closely related organisms (species and subspecies levels), for which it provided better resolution.
Journal ArticleDOI
Minimum information about a marker gene sequence (MIMARKS) and minimum information about any (x) sequence (MIxS) specifications.
Pelin Yilmaz,Pelin Yilmaz,Renzo Kottmann,Dawn Field,Rob Knight,Rob Knight,James R. Cole,Linda A. Amaral-Zettler,Jack A. Gilbert,Jack A. Gilbert,Jack A. Gilbert,Ilene Karsch-Mizrachi,Anjanette Johnston,Guy Cochrane,Robert Vaughan,Christopher I. Hunter,Joonhong Park,Norman Morrison,Philippe Rocca-Serra,Peter Sterk,Manimozhiyan Arumugam,Mark J. Bailey,Laura K. Baumgartner,Bruce W. Birren,Martin J. Blaser,Vivien Bonazzi,Timothy F. Booth,Peer Bork,Frederic D. Bushman,Pier Luigi Buttigieg,Pier Luigi Buttigieg,Patrick S. G. Chain,Patrick S. G. Chain,Patrick S. G. Chain,Emily S. Charlson,Elizabeth K. Costello,Heather Huot-Creasy,Peter Dawyndt,Todd Z. DeSantis,Noah Fierer,Jed A. Fuhrman,Rachel E. Gallery,Dirk Gevers,Richard A. Gibbs,Inigo San Gil,Antonio Gonzalez,Jeffrey I. Gordon,Robert P. Guralnick,Wolfgang Hankeln,Wolfgang Hankeln,Sarah K. Highlander,Philip Hugenholtz,Janet K. Jansson,Janet K. Jansson,Andrew L. Kau,Scott T. Kelley,Jerry Kennedy,Dan Knights,Omry Koren,Justin Kuczynski,Nikos C. Kyrpides,Robert Larsen,Christian L. Lauber,Teresa M. Legg,Ruth E. Ley,Catherine A. Lozupone,Wolfgang Ludwig,Donna Lyons,Eamonn Maguire,Barbara A. Methé,Folker Meyer,Brian D. Muegge,Sara Nakielny,Karen E. Nelson,Diana R. Nemergut,Josh D. Neufeld,Lindsay K. Newbold,Anna Oliver,Norman R. Pace,Giriprakash Palanisamy,Jörg Peplies,Joseph F. Petrosino,Lita M. Proctor,Elmar Pruesse,Elmar Pruesse,Christian Quast,Jeroen Raes,Sujeevan Ratnasingham,Jacques Ravel,David A. Relman,David A. Relman,Susanna Assunta-Sansone,Patrick D. Schloss,Lynn M. Schriml,Rohini Sinha,Michelle I. Smith,Erica Sodergren,Aymé Spor,Jesse Stombaugh,James M. Tiedje,Doyle V. Ward,George M. Weinstock,Doug Wendel,Owen White,Andrew S. Whiteley,Andreas Wilke,Jennifer R. Wortman,Tanya Yatsunenko,Frank Oliver Glöckner,Frank Oliver Glöckner +109 more
TL;DR: To establish a unified standard for describing sequence data and to provide a single point of entry for the scientific community to access and learn about GSC checklists, the minimum information about any (x) sequence is presented (MIxS).
Journal ArticleDOI
Direct Determination of Carbon and Nitrogen Contents of Natural Bacterial Assemblages in Marine Environments
TL;DR: The estimates support the previous proposition that bacteria contribute substantially to total biomass in marine environments, but they also suggest that the use of a single conversion factor for diverse marine environments can lead to large errors in assessing the role of bacteria in food webs and biogeochemical cycles.
Journal ArticleDOI
A Natural View of Microbial Biodiversity within Hot Spring Cyanobacterial Mat Communities
TL;DR: This review summarizes a decade of research that finds that it may be possible to understand microbial biodiversity within hot spring cyanobacterial mats by using principles similar to those developed by evolutionary ecologists to understand biodiversity of larger species.
Journal ArticleDOI
Implications of streamlining theory for microbial ecology
TL;DR: Streamlining theory is belied by the observation that many successful bacteria are large cells with complex genomes, which means that to fully appreciate streamlining, the authors must look to the life histories and adaptive strategies of cells, which impose minimum requirements for complexity that vary with niche.
References
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