Journal ArticleDOI
Genetic diversity in Sargasso Sea bacterioplankton.
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TLDR
The phylogenetically analysed clone libraries of eubacterial 16S ribosomal RNA genes amplified from natural populations of Sargasso Sea picoplankton indicate the presence of a novel microbial group, the SAR 11 cluster, which appears to be a significant component of this oligotrophic bacterioplankton community.Abstract:
BACTERIOPLANKTON are recognized as important agents of biogeochemical change in marine ecosystems, yet relatively little is known about the species that make up these communities. Uncertainties about the genetic structure and diversity of natural bacterioplankton populations stem from the traditional difficulties associated with microbial cultivation techniques. Discrepancies between direct counts and plate counts are typically several orders of magnitude, raising doubts as to whether cultivated marine bacteria are actually representative of dominant planktonic species1–3. We have phylogenetically analysed clone libraries of eubacterial 16S ribosomal RNA genes amplified from natural populations of Sargasso Sea picoplankton by the polymerase chain reaction4. The analysis indicates the presence of a novel microbial group, the SAR 11 cluster, which appears to be a significant component of this oligotrophic bacterioplankton community. A second cluster of lineages related to the oxygenic phototrophs—cyanobacteria, prochlorophytes and chloroplasts—was also observed. However, none of the genes matched the small subunit rRNA sequences of cultivated marine cyanobacteria from similar habitats. The diversity of 16S rRNA genes observed within the clusters suggests that these bacterioplankton may be consortia of independent lineages sharing surprisingly distant common ancestors.read more
Citations
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Relationships between bacterial diversity and environmental variables in a tropical marine environment, Rio de Janeiro
Ricardo Vieira,Alessandra M. Gonzalez,Alexander M. Cardoso,Denise Neves de Oliveira,Rodolpho Mattos Albano,Maysa M. Clementino,Orlando B. Martins,Rodolfo Paranhos +7 more
TL;DR: Comparisons of environmental chemistry, microbiological parameters and bacterioplankton 16S rRNA clone libraries from different areas of a 50 km transect along a trophic gradient in the tropical Guanabara Bay ecosystem indicated that each of the libraries differs significantly from the others.
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Contribution of the Microbial Communities Detected on an Oil Painting on Canvas to Its Biodeterioration
María del Mar López-Miras,Inés Martín-Sánchez,África Yebra-Rodríguez,Julio Romero-Noguera,Fernando Bolívar-Galiano,Jörg Ettenauer,Katja Sterflinger,Guadalupe Piñar +7 more
TL;DR: The cultivable microorganisms, which exhibited enzymatic activities related to the deterioration processes, were selected to evaluate their biodeteriorative potential on canvas paintings and revealed that neither Arthrobacter sp.
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Genetic diversity in Prochlorococcus populations flow cytometrically sorted from the Sargasso Sea and Gulf Stream
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TL;DR: It appears that wild Prochlorococcus populations consist of individuals drawn from a variety of evolutionary lineages and that populations at different depths and in two distinct hydrographic rcgimcs recruit their members from the same gene pool.
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Microbial diversity in a Pacific Ocean transect from the Arctic to Antarctic circles
Amy J. Baldwin,Joseph A. Moss,Joseph D. Pakulski,Phillipe Catala,Fabien Joux,Wade H. Jeffrey +5 more
TL;DR: T-RFLP patterns suggest that microbial communities may be influenced by ambient water temperature, with mid-latitudinal and equatorial communities more similar in composition to each other than to cold water communities.
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Microbial silica deposition in geothermal hot waters.
TL;DR: A combined use of molecular ecological techniques and geochemical surveys revealed that thermophilic or hyperthermophilic microorganisms living in geothermal environments are likely to be implicated in the formation of biogenic siliceous deposits.
References
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Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
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Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Journal ArticleDOI
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
TL;DR: In this paper, complementary oligodeoxyribonucleotide (oligo) primers and the polymerase chain reaction are used to generate two DNA fragments having overlapping ends, and these fragments are combined in a subsequent 'fusion' reaction in which the overlapping ends anneal, allowing the 3' overlap of each strand to serve as a primer for the three' extension of the complementary strand.
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