Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation
Marie Toussaint,David A. Jackson,Dawid Swieboda,Anabel Guedán,Theodora-Dorita Tsourouktsoglou,Yee Man Ching,Coraline Radermecker,Heidi Makrinioti,Julia Aniscenko,Michael R. Edwards,Roberto Solari,Frédéric Farnir,Venizelos Papayannopoulos,Fabrice Bureau,Thomas Marichal,Sebastian L. Johnston +15 more
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TLDR
In a mouse model of allergic airway hypersensitivity, it is shown that rhinovirus infection triggers dsDNA release associated with the formation of neutrophil extracellular traps (NETs), known as NETosis, which contribute to the pathogenesis and may represent potential therapeutic targets of rhinOVirus-induced asthma exacerbations.Abstract:
Respiratory viral infections represent the most common cause of allergic asthma exacerbations. Amplification of the type-2 immune response is strongly implicated in asthma exacerbation, but how virus infection boosts type-2 responses is poorly understood. We report a significant correlation between the release of host double-stranded DNA (dsDNA) following rhinovirus infection and the exacerbation of type-2 allergic inflammation in humans. In a mouse model of allergic airway hypersensitivity, we show that rhinovirus infection triggers dsDNA release associated with the formation of neutrophil extracellular traps (NETs), known as NETosis. We further demonstrate that inhibiting NETosis by blocking neutrophil elastase or by degrading NETs with DNase protects mice from type-2 immunopathology. Furthermore, the injection of mouse genomic DNA alone is sufficient to recapitulate many features of rhinovirus-induced type-2 immune responses and asthma pathology. Thus, NETosis and its associated extracellular dsDNA contribute to the pathogenesis and may represent potential therapeutic targets of rhinovirus-induced asthma exacerbations.read more
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G-CSFR antagonism reduces neutrophilic inflammation during pneumococcal and influenza respiratory infections without compromising clearance
TL;DR: G-CSFR signalling contributes to neutrophil trafficking in response to two common respiratory pathogens during Streptococcus pneumoniae or influenza A virus lung infection in mice.
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Lactic Acid Accumulation During Exhaustive Exercise Impairs Release of Neutrophil Extracellular Traps in Mice.
Yue Shi,Hui Shi,David C. Nieman,Qiongyi Hu,Luyu Yang,Tingting Liu,Xiaofeng Zhu,Hongzhan Wei,Die Wu,Fei Li,Yanqiu Cui,Peijie Chen +11 more
TL;DR: The data support a negative relationship between LA accumulation and NETs release after heavy exertion, and furthers the understanding of how NETs and oxidative reaction respond to one bout of prolonged and intensive running.
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Neutrophilic inflammation in asthma and defective epithelial translational control.
TL;DR: Epithelial hyperresponsiveness was associated with failure of the translational repressor T-cell internal antigen-1 related protein (TiAR) to translocate to the cytoplasm to halt CXCL-8 production, as confirmed by TiAR knockdown, in line with the finding that hyperresponsive PBECs also produced enhanced levels of other inflammatory mediators.
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Arabidopsis thaliana Response to Extracellular DNA: Self Versus Nonself Exposure
Maria Luisa Chiusano,Maria Luisa Chiusano,Guido Incerti,Chiara Colantuono,Pasquale Termolino,Emanuela Palomba,Francesco Monticolo,Giovanna Benvenuto,Alessandro Foscari,Alfonso Esposito,Lucia Marti,Giulia De Lorenzo,Isaac Vega-Muñoz,Martin Heil,Fabrizio Cartenì,Giuliano Bonanomi,Stefano Mazzoleni +16 more
TL;DR: In this paper, a whole-plant transcriptome profiling in the model species Arabidopsis thaliana exposed to extracellular self- (conspecific) and non-self- (heterologous) DNA was performed.
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IgE Autoreactivity in Atopic Dermatitis: Paving the Road for Autoimmune Diseases?
TL;DR: A potential outcome of IgE autoreactivity in AD could be the development of further autoimmune diseases, which is associated epidemiologically with several autoimmune diseases showing autoreactive IgE secretion.
References
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Journal ArticleDOI
Neutrophil extracellular traps kill bacteria
Volker Brinkmann,Ulrike Reichard,Christian Goosmann,Beatrix Fauler,Yvonne Uhlemann,David S. Weiss,Yvette Weinrauch,Yvette Weinrauch,Arturo Zychlinsky +8 more
TL;DR: It is described that, upon activation, neutrophils release granule proteins and chromatin that together form extracellular fibers that bind Gram-positive and -negative bacteria, which degrade virulence factors and kill bacteria.
Journal ArticleDOI
Community study of role of viral infections in exacerbations of asthma in 9-11 year old children.
Sebastian L. Johnston,Philip Pattemore,G. Sanderson,Sandra Smith,F Lampe,Lynn Josephs,P. Symington,S. O'Toole,S. H. Myint,D. A. J. Tyrrell +9 more
TL;DR: This study supports the hypothesis that upper respiratory viral infections are associated with 80-85% of asthma exacerbations in school age children.
Journal ArticleDOI
Extracellular DNA traps promote thrombosis
Tobias A. Fuchs,Alexander Brill,Daniel Duerschmied,Daphne Schatzberg,Marc Monestier,Daniel D. Myers,Shirley K. Wrobleski,Thomas W. Wakefield,John H. Hartwig,Denisa D. Wagner +9 more
TL;DR: It is reported that NETs provide a heretofore unrecognized scaffold and stimulus for thrombus formation and may further explain the epidemiological association of infection with thrombosis.
Journal ArticleDOI
Plasmacytoid dendritic cells sense self-DNA coupled with antimicrobial peptide.
Roberto Lande,Josh Gregorio,Valeria Facchinetti,Bithi Chatterjee,Bithi Chatterjee,Yi Hong Wang,Bernhard Homey,Wei Cao,Yui-Hsi Wang,Bing Su,Frank O. Nestle,Tomasz Zal,Ira Mellman,Ira Mellman,Jens-Michael Schröder,Yong-Jun Liu,Michel Gilliet +16 more
TL;DR: The data uncover a fundamental role of an endogenous antimicrobial peptide in breaking innate tolerance to self-DNA and suggest that this pathway may drive autoimmunity in psoriasis.