Inhibiting glycolytic metabolism enhances CD8+ T cell memory and antitumor function
Madhusudhanan Sukumar,Jie Liu,Yun Ji,Murugan Subramanian,Joseph G. Crompton,Zhiya Yu,Rahul Roychoudhuri,Douglas C. Palmer,Pawel Muranski,Edward D. Karoly,Robert P. Mohney,Christopher A. Klebanoff,Ashish Lal,Toren Finkel,Nicholas P. Restifo,Luca Gattinoni +15 more
Reads0
Chats0
TLDR
It is indicated that augmenting glycolytic flux drives CD8+ T cells toward a terminally differentiated state, while its inhibition preserves the formation of long-lived memory CD8+, and the efficacy of T cell-based therapies against chronic infectious diseases and cancer.Abstract:
Naive CD8+ T cells rely upon oxidation of fatty acids as a primary source of energy. After antigen encounter, T cells shift to a glycolytic metabolism to sustain effector function. It is unclear, however, whether changes in glucose metabolism ultimately influence the ability of activated T cells to become long-lived memory cells. We used a fluorescent glucose analog, 2-NBDG, to quantify glucose uptake in activated CD8+ T cells. We found that cells exhibiting limited glucose incorporation had a molecular profile characteristic of memory precursor cells and an increased capacity to enter the memory pool compared with cells taking up high amounts of glucose. Accordingly, enforcing glycolytic metabolism by overexpressing the glycolytic enzyme phosphoglycerate mutase-1 severely impaired the ability of CD8+ T cells to form long-term memory. Conversely, activation of CD8+ T cells in the presence of an inhibitor of glycolysis, 2-deoxyglucose, enhanced the generation of memory cells and antitumor functionality. Our data indicate that augmenting glycolytic flux drives CD8+ T cells toward a terminally differentiated state, while its inhibition preserves the formation of long-lived memory CD8+ T cells. These results have important implications for improving the efficacy of T cell–based therapies against chronic infectious diseases and cancer.read more
Citations
More filters
Journal ArticleDOI
Metabolic Reprogramming by the Excessive AMPK Activation Exacerbates Antigen-Specific Memory CD8+ T Cell Differentiation after Acute Lymphocytic Choriomeningitis Virus Infection.
Jimin Son,Yong Woo Cho,Youn Jung Woo,Young Ae Baek,Eun Jee Kim,Yuri Cho,J.Y. Kim,Beom Seok Kim,Beom Seok Kim,Jason Jungsik Song,Sang Jun Ha +10 more
TL;DR: In vivo treatment of IM156 impaired the function of virus-specific memory CD8+ T cells, indicating that excessive AMPK activation weakens memory T cell differentiation, thereby suppressing recall immune responses.
Journal ArticleDOI
Extracellular acidosis restricts one-carbon metabolism and preserves T cell stemness
Hongcheng Cheng,Yajing Qiu,Yue Xu,Li Chen,Kaili Ma,Mengyuan Tao,Luke Frankiw,Hongli Yin,Ermei Xie,Xiaoli Pan,Jing Du,Zhe Wang,Wenshuai Zhu,Lu Chen,Lianjun Zhang,Guideng Li +15 more
TL;DR: In this paper , the authors show that prolonged exposure to acid reprograms T cell intracellular metabolism and mitochondrial fitness and preserves T cell stemness, and they also reveal an unexpected capacity of extracellular acidosis to maintain the stem-like properties of T cells.
Journal ArticleDOI
Role of CAR T Cell Metabolism for Therapeutic Efficacy
TL;DR: In this article , the role of CAR T cell metabolism in therapeutic efficacy together with potential targets of intervention is discussed, where the authors focus on metabolic modulation as a mean to improve clinical efficacy and even tolerability.
Posted ContentDOI
Digoxin Targets Central Carbon Metabolism and Remodels the Tumor Microenvironment
Sydney M. Sanderson,Zhengtao Xiao,Amy J. Wisdom,Shree Bose,Maria V. Liberti,Michael A. Reid,Emily Hocke,Simon G. Gregory,David G. Kirsch,Jason W. Locasale +9 more
TL;DR: It is suggested that digoxin suppresses tumor growth by targeting central carbon in both tumor cells and the immune compartment, rendering it promising as an antitumor agent for metabolic therapeutic and immunomodulatory applications.
BookDOI
Immune Metabolism in Health and Tumor
TL;DR: This chapter summarizes how metabolic programs are tuned and what the physiological consequences of metabolic reprogramming are as they relate to immune cell homeostasis, differentiation, and function.
References
More filters
Journal ArticleDOI
Lineage relationship and protective immunity of memory CD8 T cell subsets.
E. John Wherry,Volker Teichgräber,Todd C. Becker,David Masopust,Susan M. Kaech,Rustom Antia,Ulrich H. von Andrian,Rafi Ahmed +7 more
TL;DR: It is proposed that TCM and TEM do not necessarily represent distinct subsets, but are part of a continuum in a linear naive → effector → TEM → TCM differentiation pathway.
Journal ArticleDOI
Durable Complete Responses in Heavily Pretreated Patients with Metastatic Melanoma Using T Cell Transfer Immunotherapy
Steven A. Rosenberg,James Chih-Hsin Yang,Richard M. Sherry,Udai S. Kammula,Michael S. Hughes,Giao Q. Phan,Deborah Citrin,Nicholas P. Restifo,Paul F. Robbins,John R. Wunderlich,Kathleen E. Morton,Carolyn M. Laurencot,Seth M. Steinberg,Donald E. White,Mark E. Dudley +14 more
TL;DR: Cell transfer therapy with autologous TILs can mediate durable complete responses in patients with metastatic melanoma and has similar efficacy irrespective of prior treatment.
Journal ArticleDOI
Posttranscriptional Control of T Cell Effector Function by Aerobic Glycolysis
Chih-Hao Chang,Jonathan D. Curtis,Leonard B. Maggi,Brandon Faubert,Alejandro V. Villarino,David O’Sullivan,Stanley Ching-Cheng Huang,Gerritje J.W. van der Windt,Julianna Blagih,Jing Qiu,Jason D. Weber,Edward J. Pearce,Russell G. Jones,Erika L. Pearce +13 more
TL;DR: It is shown here that aerobic glycolysis is specifically required for effector function in T cells but that this pathway is not necessary for proliferation or survival.
Journal ArticleDOI
The transcription factor Myc controls metabolic reprogramming upon T lymphocyte activation
Ruoning Wang,Christopher P. Dillon,Lewis Zhichang Shi,Sandra Milasta,Robert Carter,David Finkelstein,Laura L. McCormick,Patrick Fitzgerald,Hongbo Chi,Joshua Munger,Douglas R. Green +10 more
TL;DR: Metabolic tracer analysis revealed a Myc-dependent metabolic pathway linking glutaminolysis to the biosynthesis of polyamines, which may represent a general mechanism for metabolic reprogramming under patho-physiological conditions.
Journal ArticleDOI
Cutting Edge: Distinct Glycolytic and Lipid Oxidative Metabolic Programs Are Essential for Effector and Regulatory CD4+ T Cell Subsets
Ryan D. Michalek,Valerie A. Gerriets,Sarah R. Jacobs,Andrew N. Macintyre,Nancie J. MacIver,Emily F. Mason,Sarah A. Sullivan,Amanda G. Nichols,Jeffrey C. Rathmell +8 more
TL;DR: Teff and Treg were selectively increased in Glut1 transgenic mice and reliant on glucose metabolism, whereas Treg had activated AMP-activated protein kinase and were dependent on lipid oxidation.