Inhibiting glycolytic metabolism enhances CD8+ T cell memory and antitumor function
Madhusudhanan Sukumar,Jie Liu,Yun Ji,Murugan Subramanian,Joseph G. Crompton,Zhiya Yu,Rahul Roychoudhuri,Douglas C. Palmer,Pawel Muranski,Edward D. Karoly,Robert P. Mohney,Christopher A. Klebanoff,Ashish Lal,Toren Finkel,Nicholas P. Restifo,Luca Gattinoni +15 more
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TLDR
It is indicated that augmenting glycolytic flux drives CD8+ T cells toward a terminally differentiated state, while its inhibition preserves the formation of long-lived memory CD8+, and the efficacy of T cell-based therapies against chronic infectious diseases and cancer.Abstract:
Naive CD8+ T cells rely upon oxidation of fatty acids as a primary source of energy. After antigen encounter, T cells shift to a glycolytic metabolism to sustain effector function. It is unclear, however, whether changes in glucose metabolism ultimately influence the ability of activated T cells to become long-lived memory cells. We used a fluorescent glucose analog, 2-NBDG, to quantify glucose uptake in activated CD8+ T cells. We found that cells exhibiting limited glucose incorporation had a molecular profile characteristic of memory precursor cells and an increased capacity to enter the memory pool compared with cells taking up high amounts of glucose. Accordingly, enforcing glycolytic metabolism by overexpressing the glycolytic enzyme phosphoglycerate mutase-1 severely impaired the ability of CD8+ T cells to form long-term memory. Conversely, activation of CD8+ T cells in the presence of an inhibitor of glycolysis, 2-deoxyglucose, enhanced the generation of memory cells and antitumor functionality. Our data indicate that augmenting glycolytic flux drives CD8+ T cells toward a terminally differentiated state, while its inhibition preserves the formation of long-lived memory CD8+ T cells. These results have important implications for improving the efficacy of T cell–based therapies against chronic infectious diseases and cancer.read more
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Nandinine, a Derivative of Berberine, Inhibits Inflammation and Reduces Insulin Resistance in Adipocytes via Regulation of AMP-Kinase Activity.
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How immune-cell fate and function are determined by metabolic pathway choice: The bioenergetics underlying the immune response.
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Rhein enhances the cytotoxicity of effector lymphocytes in colon cancer under hypoxic conditions
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can be targeted to control specific T cell populations in autoimmune and inflammatory diseases. Metabolic programming and PDHK1 control CD4 + T cell subsets and inflammation
Valerie A. Gerriets,Rigel J. Kishton,Amanda G. Nichols,Andrew N. Macintyre,Makoto Inoue,Olga Ilkayeva,Peter S. Winter,Xiaojing Liu,Bhavana Priyadharshini,Marta E. Slawinska,Lea Haeberli,Catherine Huck,Laurence A. Turka,Kris C. Wood,Laura P. Hale,Paul Smith,Martin A. Schneider,Nancie J. MacIver,Jason W. Locasale,Christopher B. Newgard,Mari L. Shinohara,Jeffrey C. Rathmell +21 more
TL;DR: Analysis of CD4+ T cell populations in murine models and metabolic profiling revealed that pyruvate dehydrogenase (PDH) is a key bifurcation point between T cell glycolytic and oxidative metabolism, showing that CD4- subsets utilize and require distinct metabolic programs that can be targeted to control specific Tcell populations in autoimmune and inflammatory diseases.
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Metabolic perturbation sensitizes human breast cancer to NK cell-mediated cytotoxicity by increasing the expression of MHC class I chain-related A/B
Dexue Fu,Jean Francois H. Geschwind,Swathi Karthikeyan,Eliyahu Miller,Rani Kunjithapatham,Zhijun Wang,Shanmugasundaram Ganapathy-Kanniappan +6 more
TL;DR: In this article, metabolic perturbation by the glycolytic inhibitor, 3-bromopyruvate (3-BrPA), augments the surface expression of MICA/B in human breast cancer cell lines.
References
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Lineage relationship and protective immunity of memory CD8 T cell subsets.
E. John Wherry,Volker Teichgräber,Todd C. Becker,David Masopust,Susan M. Kaech,Rustom Antia,Ulrich H. von Andrian,Rafi Ahmed +7 more
TL;DR: It is proposed that TCM and TEM do not necessarily represent distinct subsets, but are part of a continuum in a linear naive → effector → TEM → TCM differentiation pathway.
Journal ArticleDOI
Durable Complete Responses in Heavily Pretreated Patients with Metastatic Melanoma Using T Cell Transfer Immunotherapy
Steven A. Rosenberg,James Chih-Hsin Yang,Richard M. Sherry,Udai S. Kammula,Michael S. Hughes,Giao Q. Phan,Deborah Citrin,Nicholas P. Restifo,Paul F. Robbins,John R. Wunderlich,Kathleen E. Morton,Carolyn M. Laurencot,Seth M. Steinberg,Donald E. White,Mark E. Dudley +14 more
TL;DR: Cell transfer therapy with autologous TILs can mediate durable complete responses in patients with metastatic melanoma and has similar efficacy irrespective of prior treatment.
Journal ArticleDOI
Posttranscriptional Control of T Cell Effector Function by Aerobic Glycolysis
Chih-Hao Chang,Jonathan D. Curtis,Leonard B. Maggi,Brandon Faubert,Alejandro V. Villarino,David O’Sullivan,Stanley Ching-Cheng Huang,Gerritje J.W. van der Windt,Julianna Blagih,Jing Qiu,Jason D. Weber,Edward J. Pearce,Russell G. Jones,Erika L. Pearce +13 more
TL;DR: It is shown here that aerobic glycolysis is specifically required for effector function in T cells but that this pathway is not necessary for proliferation or survival.
Journal ArticleDOI
The transcription factor Myc controls metabolic reprogramming upon T lymphocyte activation
Ruoning Wang,Christopher P. Dillon,Lewis Zhichang Shi,Sandra Milasta,Robert Carter,David Finkelstein,Laura L. McCormick,Patrick Fitzgerald,Hongbo Chi,Joshua Munger,Douglas R. Green +10 more
TL;DR: Metabolic tracer analysis revealed a Myc-dependent metabolic pathway linking glutaminolysis to the biosynthesis of polyamines, which may represent a general mechanism for metabolic reprogramming under patho-physiological conditions.
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Cutting Edge: Distinct Glycolytic and Lipid Oxidative Metabolic Programs Are Essential for Effector and Regulatory CD4+ T Cell Subsets
Ryan D. Michalek,Valerie A. Gerriets,Sarah R. Jacobs,Andrew N. Macintyre,Nancie J. MacIver,Emily F. Mason,Sarah A. Sullivan,Amanda G. Nichols,Jeffrey C. Rathmell +8 more
TL;DR: Teff and Treg were selectively increased in Glut1 transgenic mice and reliant on glucose metabolism, whereas Treg had activated AMP-activated protein kinase and were dependent on lipid oxidation.