Journal ArticleDOI
Intrinsically unstructured proteins: re-assessing the protein structure-function paradigm.
Peter E. Wright,H. Jane Dyson +1 more
TLDR
Many proteins that lack intrinsic globular structure under physiological conditions have now been recognized, and it appears likely that their rapid turnover, aided by their unstructured nature in the unbound state, provides a level of control that allows rapid and accurate responses of the cell to changing environmental conditions.About:
This article is published in Journal of Molecular Biology.The article was published on 1999-10-22. It has received 2804 citations till now. The article focuses on the topics: Protein structure function & Intrinsically disordered proteins.read more
Citations
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Intrinsically disordered proteins in the nucleus of human cells.
Telma Frege,Vladimir N. Uversky +1 more
TL;DR: Information is gathered about proteins that exist in various compartments and sub-nuclear bodies of the nucleus of the human cells, with the goal of identifying which ones are highly disordered and which functions are ascribed to the disordered nuclear proteins.
Journal ArticleDOI
Functional interactions of nucleocapsid protein of feline immunodeficiency virus and cellular prion protein with the viral RNA.
Mila Moscardini,Mauro Pistello,Mauro Bendinelli,Damien Ficheux,Jennifer T. Miller,Caroline Gabus,Stuart F. J. Le Grice,Witold K. Surewicz,Jean-Luc Darlix +8 more
TL;DR: An in vitro system to investigate replication of the feline immunodeficiency virus (FIV), which is functionally and phylogenetically distant from HIV-1, shows that NC protein chaperones viral RNA dimerization, primer tRNA annealing to the genomic primer-binding site (PBS) and minus strand DNA synthesis by the homologous FIV RT.
Journal ArticleDOI
Biophysical Characterization of the Unstructured Cytoplasmic Domain of the Human Neuronal Adhesion Protein Neuroligin 3
Aviv Paz,Tzviya Zeev-Ben-Mordehai,Martin Lundqvist,Eilon Sherman,Efstratios Mylonas,Lev Weiner,Gilad Haran,Dmitri I. Svergun,Frans A. A. Mulder,Joel L. Sussman,Israel Silman +10 more
TL;DR: It is shown that the cytoplasmic domain of human neuroligin 3 is intrinsically unstructured, however, several of these techniques indicate that it is not fully extended, but becomes significantly more extended under denaturing conditions.
Journal ArticleDOI
Thermodynamics of the Op18/Stathmin-Tubulin Interaction
TL;DR: Findings do not support the view that stathmin works as a pH-sensitive protein and suggest that the hydrophobic effect is the major driving force of the binding reaction.
Journal ArticleDOI
Mechanism of Folding and Binding of an Intrinsically Disordered Protein As Revealed by ab Initio Simulations.
TL;DR: It is found that a key folding and binding step is linked to the formation of weak native interactions between a preformed nativelike fragment of a pKID helix and KIX surface, reminiscent of a nucleation-condensation model, a common scenario for folding of globular proteins.
References
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The Transcriptional Coactivators p300 and CBP Are Histone Acetyltransferases
TL;DR: It is demonstrated that p300/CBP acetylates nucleosomes in concert with PCAF, a novel class of acetyltransferases in that it does not have the conserved motif found among various other acetyl transferases.
Journal ArticleDOI
The Structural Basis of Estrogen Receptor/Coactivator Recognition and the Antagonism of This Interaction by Tamoxifen
Andrew K. Shiau,Danielle Barstad,Paula M. Loria,Lin Cheng,Peter J. Kushner,David A. Agard,Geoffrey L. Greene +6 more
TL;DR: Crystal structures of the human estrogen receptor alpha (hER alpha) ligand-binding domain (LBD) and the OHT-LBD complex reveal the two distinct mechanisms by which structural features of OHT promote this "autoinhibitory" helix 12 conformation.
Journal ArticleDOI
Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution
TL;DR: The X-ray crystal structure of a core synaptic fusion complex containing syntaxin-1A, synaptobrevin-II and SNAP-25B reveals a highly twisted and parallel four-helix bundle that differs from the bundles described for the haemagglutinin and HIV/SIV gp41 membrane-fusion proteins.
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Conversion of alpha-helices into beta-sheets features in the formation of the scrapie prion proteins.
Keh-Ming Pan,Michael J. Baldwin,J Nguyen,María Gasset,Ana Serban,Darlene Groth,Ingrid Mehlhorn,Ziwei Huang,Robert J. Fletterick,Fred E. Cohen +9 more
TL;DR: It is argued that the conversion of alpha-helices into beta-sheets underlies the formation of PrPSc, and it is likely that this conformational transition is a fundamental event in the propagation of prions.
Journal ArticleDOI
A signature motif in transcriptional co-activators mediates binding to nuclear receptors.
TL;DR: It is proposed that the LXXLL motif is a signature sequence that facilitates the interaction of different proteins with nuclear receptors, and is thus a defining feature of a new family of nuclear proteins.