Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: a cross-sectional study
Ximena Villamizar,Adriana Higuera,Giovanny Herrera,Luis Reinel Vasquez-A,Lorena Buitron,Lina Maria Muñoz,Fabiola E. Gonzalez-C,Myriam Consuelo López,Julio Cesar Giraldo,Juan David Ramírez +9 more
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TLDR
A descriptive cross-sectional study in school-age children attending a daycare institution in commune eight of Popayán, Cauca (Southwest Colombia) revealed the usefulness of molecular methods to depict the transmission dynamics of parasitic protozoa in southwest Colombia.Abstract:
Parasitic infections, particularly those caused by protozoa, represent a considerable public health problem in developing countries. Blastocystis, Giardia duodenalis, Cryptosporidium spp. and the Entamoeba complex (Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii) are the most common etiological causes of intestinal parasitic infections. We carried out a descriptive cross-sectional study in school-age children attending a daycare institution in commune eight of Popayan, Cauca (Southwest Colombia). A total of 266 fecal samples were collected (258 from children and eight from pets). Blastocystis, G. duodenalis, Cryptosporidium spp. and the Entamoeba complex were identified by microscopy, quantitative real-time PCR (qPCR) and conventional PCR. The concordance of qPCR and microscopy was assessed using the Kappa index. Molecular characterization was conducted to identify Blastocystis subtypes (18S), G. duodenalis assemblages (tpi and gdh) and Cryptosporidium species/subtypes (18S and GP60). Potential associations between intestinal parasitism and sociodemographic factors were examined using bivariate analyses. A total of 258 fecal samples from children were analyzed by microscopy and 255 samples were analyzed by qPCR. The prevalence of Blastocystis was between 25.19% (microscopy) and 39.22% (qPCR), that of G. duodenalis was between 8.14% (microscopy) and 10.59% (qPCR), that of Cryptosporidium spp. was estimated at 9.8% (qPCR), and that of the Entamoeba complex was between 0.39% (conventional PCR) and 0.78% (microscopy). The concordance between microscopy and qPCR was very low. Blastocystis ST1 (alleles 4, 8, and 80), ST2 (alleles 11, 12, and 15), ST3 (alleles 31, 34, 36, 38,57, and 151), and ST4 (alleles 42 and 91), G. duodenalis assemblages AII, BIII, BIV and D, C. parvum subtype IIa and C. hominis subtype IbA9G3R2 were identified. The only identified member of the Entamoeba complex corresponded to E. histolytica. No statistically significant association was identified between parasitic infection and any sociodemographic variable. This study revealed the usefulness of molecular methods to depict the transmission dynamics of parasitic protozoa in southwest Colombia. The presence of some of these protozoa in domestic animals may be involved in their transmission.read more
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Blastocystis in domesticated and wild mammals and birds.
TL;DR: This review provides a comprehensive overview of Blastocystis in animals highlighting the subtype diversity of this widespread and genetically diverse parasite in different animal groups and suggesting water can be a potential vehicle in the transmission of this parasite not only for humans but also for animals.
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Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing.
Adriana Higuera,Giovanny Herrera,Paula Jimenez,Diego José García-Corredor,Martín Orlando Pulido-Medellín,Diana M. Bulla-Castañeda,Juan Carlos Pinilla,Darwin A. Moreno-Pérez,Jenny G. Maloney,Mónica Santín,Juan David Ramírez +10 more
TL;DR: In this article, the authors characterized the frequency and subtypes of Blastocystis in domestic animals including pigs, minipigs, cows, dogs, horses, goats, sheep, and llama from three departments of Colombia.
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Prevalence and subtype distribution of Blastocystis sp. infecting children from a rural community in Panama.
Milixa Perea,Vanessa Vásquez,Vanessa Pineda,Franklyn Samudio,José E. Calzada,José E. Calzada,Azael Saldaña,Azael Saldaña +7 more
TL;DR: The results highlight the need to further investigate clinical, epidemiological, and genetic characteristics of Blastocystis sp.
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Molecular detection and genotyping of intestinal protozoa from different biogeographical regions of Colombia
Adriana Higuera,Ximena Villamizar,Giovanny Herrera,Julio Cesar Giraldo,Luis Reinel Vasquez-A,Plutarco Urbano,Oswaldo Villalobos,Catalina Tovar,Juan David Ramírez +8 more
TL;DR: High and active transmission was apparent in several regions of Colombia, implying that mechanisms for prevention and control of intestinal parasitosis in different parts of the country must be improved.
Journal ArticleDOI
Epidemiological and Molecular Characterization of Blastocystis Infection in Children Attending Daycare Centers in Medellín, Colombia.
Maria I. Osorio-Pulgarin,Adriana Higuera,Juan C. Beltran-Álzate,Miryan Sánchez-Jiménez,Juan David Ramírez +4 more
TL;DR: In this paper, the authors performed an epidemiological and molecular characterization of Blastocystis infection in a child population attending daycare centers of Medellin, Colombia, where a total of 265 children aged 0-5 years were enrolled in five children's centers in urban sectors in northwestern Colombia.
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