mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.
Zijun Wang,Fabian Schmidt,Yiska Weisblum,Frauke Muecksch,Christopher O. Barnes,Shlomo Finkin,Dennis Schaefer-Babajew,Melissa Cipolla,Christian Gaebler,Jenna Ariel Lieberman,Thiago Y. Oliveira,Zhi Yang,Morgan E. Abernathy,Kathryn E. Huey-Tubman,Arlene Hurley,Martina Turroja,Kamille A. West,Kristie Gordon,Katrina G. Millard,Victor A. Ramos,Justin Da Silva,Jianliang Xu,Robert A. Colbert,Roshni Patel,Juan Dizon,Cecille Unson-O'Brien,Irina Shimeliovich,Anna Gazumyan,Marina Caskey,Pamela J. Bjorkman,Rafael Casellas,Theodora Hatziioannou,Paul D. Bieniasz,Paul D. Bieniasz,Michel C. Nussenzweig,Michel C. Nussenzweig +35 more
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This paper reported on the antibody and memory B-cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer-BioNTech (BNT162b2) vaccine against SARS-CoV-2.Abstract:
Here we report on the antibody and memory B cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer–BioNTech (BNT162b2) vaccine against SARS-CoV-21–4 Eight weeks after the second injection of vaccine, volunteers showed high levels of IgM and IgG anti-SARS-CoV-2 spike protein (S) and receptor-binding-domain (RBD) binding titre Moreover, the plasma neutralizing activity and relative numbers of RBD-specific memory B cells of vaccinated volunteers were equivalent to those of individuals who had recovered from natural infection5,6 However, activity against SARS-CoV-2 variants that encode E484K-, N501Y- or K417N/E484K/N501-mutant S was reduced by a small—but significant—margin The monoclonal antibodies elicited by the vaccines potently neutralize SARS-CoV-2, and target a number of different RBD epitopes in common with monoclonal antibodies isolated from infected donors5–8 However, neutralization by 14 of the 17 most-potent monoclonal antibodies that we tested was reduced or abolished by the K417N, E484K or N501Y mutation Notably, these mutations were selected when we cultured recombinant vesicular stomatitis virus expressing SARS-CoV-2 S in the presence of the monoclonal antibodies elicited by the vaccines Together, these results suggest that the monoclonal antibodies in clinical use should be tested against newly arising variants, and that mRNA vaccines may need to be updated periodically to avoid a potential loss of clinical efficacy The Moderna (mRNA-1273) and Pfizer–BioNTech (BNT162b2) vaccines elicit anti-RBD antibodies similar to those elicited through natural infection with SARS-CoV-2, but their potent neutralizing activity was reduced or abolished by new viral variants of concernread more
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Vaccine breakthrough infections in veterans hospitalized with coronavirus infectious disease-2019: A case series.
Paul S. Kim,Richard Schildhouse,Sanjay Saint,Suzanne F. Bradley,Stephen W. Chensue,Nathan Houchens,Ashwin Gupta +6 more
TL;DR: In this article, the authors reviewed ten individuals hospitalized with vaccine breakthrough infections to identify patient risk factors and serologic responses upon admission, based on lab testing, infections were defined as acute infection or resolving/resolved infection.
Journal ArticleDOI
Vaccine breakthrough infections in veterans hospitalized with coronavirus infectious disease-2019: A case series.
TL;DR: In this paper , the authors reviewed 10 individuals hospitalized with vaccine breakthrough infections to identify patient risk factors and serologic responses upon admission, based on lab testing, infections were defined as acute infection or resolving/resolved infection.
Journal ArticleDOI
Spike-directed vaccination elicits robust spike-specific T-cell response, including to mutant strains.
Maja Stanojevic,Ashley Geiger,Brita Ostermeier,Danielle Sohai,Christopher A. Lazarski,Haili Lang,Mariah Jensen-Wachspress,Kathleen Webber,Peter D. Burbelo,Jeffrey I. Cohen,Michael D. Keller,Catherine M. Bollard,Conrad Russell Y. Cruz +12 more
TL;DR: Evaluated T cell responses in seronegative donors before and after vaccination to define responses to SARS-CoV-2 reference strain, as well as to mutations in the variant strains B.1.7 and B. 1.351, and observed enhanced Tcell responses to reference and variant Spike strains post vaccination.
Posted ContentDOI
Household transmission of SARS-CoV-2 R.1 lineage with spike E484K mutation in Japan
Hirotsu Y,Masao Omata +1 more
TL;DR: In this paper, the authors investigated SARS-CoV-2 emerging lineage harboring variants in receptor binding domain (RBD) of spike protein in Japan and found that the R.1 lineage was not globally predominant as of March 5, 2021.
Journal ArticleDOI
Rise and Fall of SARS-CoV-2 Lineage A.27 in Germany.
Sébastien Calvignac-Spencer,Matthias Budt,Matthew Huska,Hugues Richard,Luca Leipold,Linus Grabenhenrich,Torsten Semmler,Max von Kleist,Stefan Kröger,Thorsten Wolff,Martin Hölzer +10 more
TL;DR: In this article, the authors report on the increasing frequency of the SARS-CoV-2 lineage A.1.27 in Germany during the first months of 2021. And they show that the A.27 lineage shows a mutational pattern typical of VOIs/VOCs, including an accumulation of amino acid substitutions in the Spike glycoprotein.
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