mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.
Zijun Wang,Fabian Schmidt,Yiska Weisblum,Frauke Muecksch,Christopher O. Barnes,Shlomo Finkin,Dennis Schaefer-Babajew,Melissa Cipolla,Christian Gaebler,Jenna Ariel Lieberman,Thiago Y. Oliveira,Zhi Yang,Morgan E. Abernathy,Kathryn E. Huey-Tubman,Arlene Hurley,Martina Turroja,Kamille A. West,Kristie Gordon,Katrina G. Millard,Victor A. Ramos,Justin Da Silva,Jianliang Xu,Robert A. Colbert,Roshni Patel,Juan Dizon,Cecille Unson-O'Brien,Irina Shimeliovich,Anna Gazumyan,Marina Caskey,Pamela J. Bjorkman,Rafael Casellas,Theodora Hatziioannou,Paul D. Bieniasz,Paul D. Bieniasz,Michel C. Nussenzweig,Michel C. Nussenzweig +35 more
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This paper reported on the antibody and memory B-cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer-BioNTech (BNT162b2) vaccine against SARS-CoV-2.Abstract:
Here we report on the antibody and memory B cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer–BioNTech (BNT162b2) vaccine against SARS-CoV-21–4 Eight weeks after the second injection of vaccine, volunteers showed high levels of IgM and IgG anti-SARS-CoV-2 spike protein (S) and receptor-binding-domain (RBD) binding titre Moreover, the plasma neutralizing activity and relative numbers of RBD-specific memory B cells of vaccinated volunteers were equivalent to those of individuals who had recovered from natural infection5,6 However, activity against SARS-CoV-2 variants that encode E484K-, N501Y- or K417N/E484K/N501-mutant S was reduced by a small—but significant—margin The monoclonal antibodies elicited by the vaccines potently neutralize SARS-CoV-2, and target a number of different RBD epitopes in common with monoclonal antibodies isolated from infected donors5–8 However, neutralization by 14 of the 17 most-potent monoclonal antibodies that we tested was reduced or abolished by the K417N, E484K or N501Y mutation Notably, these mutations were selected when we cultured recombinant vesicular stomatitis virus expressing SARS-CoV-2 S in the presence of the monoclonal antibodies elicited by the vaccines Together, these results suggest that the monoclonal antibodies in clinical use should be tested against newly arising variants, and that mRNA vaccines may need to be updated periodically to avoid a potential loss of clinical efficacy The Moderna (mRNA-1273) and Pfizer–BioNTech (BNT162b2) vaccines elicit anti-RBD antibodies similar to those elicited through natural infection with SARS-CoV-2, but their potent neutralizing activity was reduced or abolished by new viral variants of concernread more
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Real-time allelic assays of SARS-CoV-2 variants to enhance sewage surveillance
Xiaoqing Xu,Yu Deng,Jiahui Ding,Xiawan Zheng,Shuxian Li,Lei Liu,H. K. Chui,Leo L. M. Poon,Tong Zhang +8 more
TL;DR: In this article , the authors designed eight assays based on allele-specific RT-qPCR for real-time allelic discrimination of eight SARS-CoV-2 variants (Alpha, Beta, Gamma, Delta, Omicron, Lambda, Mu, and Kappa) in sewage.
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Structural mechanism of SARS-CoV-2 neutralization by two murine antibodies targeting the RBD.
John M. Errico,Haiyan Zhao,Rita E. Chen,Zhuoming Liu,James Brett Case,Meisheng Ma,Aaron J. Schmitz,Michael J. Rau,James A. J. Fitzpatrick,Pei Yong Shi,Michael S. Diamond,Sean P. J. Whelan,Ali H. Ellebedy,Daved H. Fremont +13 more
TL;DR: In this paper, the authors used cryoelectron microscopy (cryo-EM) to characterize two anti-SARS-CoV-2 murine monoclonal antibodies (mAbs) in complex with spike protein, revealing similarities between epitopes targeted by human and murine B cells.
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Protection from SARS-CoV-2 Delta one year after mRNA-1273 vaccination in nonhuman primates is coincident with an anamnestic antibody response in the lower airway
Matthew Gagne,Kizzmekia S. Corbett,Kizzmekia S. Corbett,Barbara J. Flynn,Kathryn E. Foulds,Danielle A. Wagner,Shayne F. Andrew,John-Paul Todd,Christopher Cole Honeycutt,Lauren McCormick,Saule T. Nurmukhambetova,Meredith E. Davis-Gardner,Laurent Pessaint,Kevin W. Bock,Bianca M. Nagata,Mahnaz Minai,Anne P. Werner,Juan I. Moliva,Courtney Tucker,Cynthia G. Lorang,Bingchun Zhao,Elizabeth McCarthy,Anthony L. Cook,Alan Dodson,Prakriti Mudvari,Jesmine Roberts-Torres,Farida Laboune,Lingshu Wang,Adrienne Goode,Swagata Kar,Seyhan Boyoglu-Barnum,Eun Sung Yang,Wei Shi,Aurélie Ploquin,Nicole A. Doria-Rose,Andrea Carfi,John R. Mascola,Eli Boritz,Darin K. Edwards,Hanne Leth Andersen,Mark G. Lewis,Mehul S. Suthar,Barney S. Graham,Mario Roederer,Ian N. Moore,Martha Nason,Nancy J. Sullivan,Daniel C. Douek,Robert A. Seder +48 more
TL;DR: In this article, the impact of durability of immune responses on protection against SARS-CoV-2 Delta wanes over time; however, there are limited data on the effect of immunization efficacy on protection.
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Investigations on SARS-CoV-2 Susceptibility of Domestic and Wild Animals Using Primary Cell Culture Models Derived from the Upper and Lower Respiratory Tract
Iris Färber,J. Krüger,Cheila Rocha,Federico Armando,Maren von Köckritz-Blickwede,Stefan Pöhlmann,Armin Braun,Wolfgang Baumgärtner,Sandra Runft,Nadine Krüger +9 more
TL;DR: The experimental infection of primary respiratory tract cell cultures, as well as an analysis of entry-factor distribution, enable screening for SARS-CoV-2 animal reservoirs.
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Distinct neutralization profile of spike variants by antibodies induced upon SARS-CoV-2 infection or vaccination.
Margherita Rosati,Evangelos Terpos,Mahesh Agarwal,Vangelis Karalis,Jenifer Bear,Robert Burns,Xintao Hu,Demetrios Papademetriou,Ioannis Ntanasis-Stathopoulos,Ioannis P. Trougakos,Meletios-Athanasios Dimopoulos,George N. Pavlakis,Barbara K. Felber +12 more
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