mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.
Zijun Wang,Fabian Schmidt,Yiska Weisblum,Frauke Muecksch,Christopher O. Barnes,Shlomo Finkin,Dennis Schaefer-Babajew,Melissa Cipolla,Christian Gaebler,Jenna Ariel Lieberman,Thiago Y. Oliveira,Zhi Yang,Morgan E. Abernathy,Kathryn E. Huey-Tubman,Arlene Hurley,Martina Turroja,Kamille A. West,Kristie Gordon,Katrina G. Millard,Victor A. Ramos,Justin Da Silva,Jianliang Xu,Robert A. Colbert,Roshni Patel,Juan Dizon,Cecille Unson-O'Brien,Irina Shimeliovich,Anna Gazumyan,Marina Caskey,Pamela J. Bjorkman,Rafael Casellas,Theodora Hatziioannou,Paul D. Bieniasz,Paul D. Bieniasz,Michel C. Nussenzweig,Michel C. Nussenzweig +35 more
Reads0
Chats0
TLDR
This paper reported on the antibody and memory B-cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer-BioNTech (BNT162b2) vaccine against SARS-CoV-2.Abstract:
Here we report on the antibody and memory B cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer–BioNTech (BNT162b2) vaccine against SARS-CoV-21–4 Eight weeks after the second injection of vaccine, volunteers showed high levels of IgM and IgG anti-SARS-CoV-2 spike protein (S) and receptor-binding-domain (RBD) binding titre Moreover, the plasma neutralizing activity and relative numbers of RBD-specific memory B cells of vaccinated volunteers were equivalent to those of individuals who had recovered from natural infection5,6 However, activity against SARS-CoV-2 variants that encode E484K-, N501Y- or K417N/E484K/N501-mutant S was reduced by a small—but significant—margin The monoclonal antibodies elicited by the vaccines potently neutralize SARS-CoV-2, and target a number of different RBD epitopes in common with monoclonal antibodies isolated from infected donors5–8 However, neutralization by 14 of the 17 most-potent monoclonal antibodies that we tested was reduced or abolished by the K417N, E484K or N501Y mutation Notably, these mutations were selected when we cultured recombinant vesicular stomatitis virus expressing SARS-CoV-2 S in the presence of the monoclonal antibodies elicited by the vaccines Together, these results suggest that the monoclonal antibodies in clinical use should be tested against newly arising variants, and that mRNA vaccines may need to be updated periodically to avoid a potential loss of clinical efficacy The Moderna (mRNA-1273) and Pfizer–BioNTech (BNT162b2) vaccines elicit anti-RBD antibodies similar to those elicited through natural infection with SARS-CoV-2, but their potent neutralizing activity was reduced or abolished by new viral variants of concernread more
Citations
More filters
Journal ArticleDOI
Response to COVID-19 booster vaccinations in seronegative people with multiple sclerosis
Emma C. Tallantyre,Martin J. Scurr,Nicola Vickaryous,Aidan Richards,Valerie Anderson,David Baker,R. A. Chance,Nikos Evangelou,Katila George,Gavin Giovannoni,Katharine Harding,Aimee Hibbert,Gillian Ingram,Stephen Jolles,Meleri Jones,Andrew H. Kang,Samantha Loveless,Stuart J. Moat,N. A. Robertson,Francesca M. Rios,Klaus Schmierer,Mark Willis,Andrew James Godkin,Ruth Dobson +23 more
TL;DR: In this article , Humoral and T cell responses to SARS-CoV-2 spike protein and nucleocapsid antigen were measured, and almost 8 out of 10 had a measurable immune response following 3rd COVID-19 vaccine.
Journal ArticleDOI
Effects of Spike Mutations in SARS-CoV-2 Variants of Concern on Human or Animal ACE2-Mediated Virus Entry and Neutralization
Yunjeong Kim,Natasha N. Gaudreault,David A. Meekins,Krishani Dinali Perera,Dashzeveg Bold,Jessie D. Trujillo,Igor Morozov,Chester McDowell,Kyeong-Ok Chang,Jürgen A. Richt +9 more
TL;DR: Important insights are revealed into the host range of SARS-CoV-2 and the effect of recently emergent S protein substitutions on viral entry, virus replication, and antibody-mediated viral neutralization.
Journal ArticleDOI
Previous SARS-CoV-2 Infection Increases B.1.1.7 Cross-Neutralization by Vaccinated Individuals.
Benjamin Trinité,Edwards Pradenas,Silvia Marfil,Carla Rovirosa,Victor Urrea,Ferran Tarrés-Freixas,Raquel Ortiz,Jordi Rodon,Júlia Vergara-Alert,Joaquim Segalés,Victor Guallar,Rosalba Lepore,Nuria Izquierdo-Useros,Gloria Trujillo,Jaume Trapé,Carolina González-Fernández,Antonia Flor,Rafel Perez-Vidal,Ruth Toledo,Anna Chamorro,Roger Paredes,Ignacio Blanco,Eulalia Grau,Marta Massanella,Jorge Carrillo,Bonaventura Clotet,Julià Blanco +26 more
TL;DR: In this article, the neutralizing activity of infected and/or vaccinated individuals against pseudoviruses expressing the spike of the original SARS-CoV-2 isolate Wuhan-Hu-1, the D614G mutant and the B.1.7 variant was evaluated.
Posted ContentDOI
Complete Protection of Nasal and Lung Airways Against SARS-CoV-2 Challenge by Antibody Plus Th1 Dominant N- and S-Specific T-Cell Responses to Subcutaneous Prime and Thermally-Stable Oral Boost Bivalent hAd5 Vaccination in an NHP Study
Elizabeth Gabitzsch,Jeffrey T. Safrit,Mohit Verma,Adrian Rice,Peter A. Sieling,Lise Zakin,Annie Shin,Brett Morimoto,Helty Adisetiyo,Raymond C.B. Wong,Ashish Bezawada,Kyle Dinkins,Joseph P. Balint,Victor Peykov,Hermes Garban,Philip T. Liu,Andrew Bacon,Jeff Drew,Patricia Spilman,Lennie Sender,Shahrooz Rabizadeh,Kayvan Niazi,Patrick Soon-Shiong +22 more
TL;DR: The hAd5 spike + nucleocapsid SC prime/oral boost vaccine elicits humoral and T-cell responses and protects rhesus macaques from SARS-CoV-2 challenge.
Posted ContentDOI
High genetic barrier to escape from human polyclonal SARS-CoV-2 neutralizing antibodies
Fabian Schmidt,Yiska Weisblum,Magdalena Rutkowska,Daniel Poston,Justin Da Silva,Fengwen Zhang,Eva Bednarski,Alice Cho,Dennis Schaefer-Babajew,Christian Gaebler,Marina Caskey,Michel C. Nussenzweig,Michel C. Nussenzweig,Theodora Hatziioannou,Paul D. Bieniasz,Paul D. Bieniasz +15 more
TL;DR: In this paper, the number and variability of the neutralizing epitopes targeted by polyclonal antibodies in SARS-CoV-2 convalescent and vaccinated individuals are key determinants of neutralization breadth and, consequently, the genetic barrier to viral escape.
References
More filters
Journal ArticleDOI
Features and development of Coot.
TL;DR: Coot is a molecular-graphics program designed to assist in the building of protein and other macromolecular models and the current state of development and available features are presented.
Journal ArticleDOI
A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
Journal ArticleDOI
Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine.
Fernando P. Polack,Stephen J. Thomas,Nicholas Kitchin,Judith Absalon,Alejandra Gurtman,Stephen Lockhart,John L. Perez,Gonzalo Pérez Marc,Edson D. Moreira,Cristiano Zerbini,Ruth Bailey,Kena A. Swanson,Satrajit Roychoudhury,Kenneth Koury,Ping Li,Warren Kalina,David A. Cooper,Robert W. Frenck,Laura L. Hammitt,Özlem Türeci,Haylene Nell,Axel Schaefer,Serhat Ünal,Dina B. Tresnan,Susan Mather,Philip R. Dormitzer,Ugur Sahin,Kathrin U. Jansen,William C. Gruber +28 more
TL;DR: A two-dose regimen of BNT162b2 conferred 95% protection against Covid-19 in persons 16 years of age or older and safety over a median of 2 months was similar to that of other viral vaccines.
Journal ArticleDOI
cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination
TL;DR: It is shown that stochastic gradient descent (SGD) and branch-and-bound maximum likelihood optimization algorithms permit the major steps in cryo-EM structure determination to be performed in hours or minutes on an inexpensive desktop computer.
Journal ArticleDOI
Automated electron microscope tomography using robust prediction of specimen movements.
TL;DR: A new method was developed to acquire images automatically at a series of specimen tilts, as required for tomographic reconstruction, using changes in specimen position at previous tilt angles to predict the position at the current tilt angle.
Related Papers (5)
Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine.
Fernando P. Polack,Stephen J. Thomas,Nicholas Kitchin,Judith Absalon,Alejandra Gurtman,Stephen Lockhart,John L. Perez,Gonzalo Pérez Marc,Edson D. Moreira,Cristiano Zerbini,Ruth Bailey,Kena A. Swanson,Satrajit Roychoudhury,Kenneth Koury,Ping Li,Warren Kalina,David A. Cooper,Robert W. Frenck,Laura L. Hammitt,Özlem Türeci,Haylene Nell,Axel Schaefer,Serhat Ünal,Dina B. Tresnan,Susan Mather,Philip R. Dormitzer,Ugur Sahin,Kathrin U. Jansen,William C. Gruber +28 more
Efficacy and Safety of the mRNA-1273 SARS-CoV-2 Vaccine.
Lindsey R. Baden,Hana M. El Sahly,Brandon Essink,Karen L. Kotloff,Sharon E. Frey,Rick Novak,David Diemert,Stephen A. Spector,Nadine Rouphael,C. Buddy Creech,John W McGettigan,Shishir Khetan,Nathan Segall,Joel Solis,Adam Brosz,Carlos Fierro,Howard J. Schwartz,Kathleen M. Neuzil,Lawrence Corey,Peter B. Gilbert,Holly Janes,Dean Follmann,Mary A. Marovich,John R. Mascola,Laura Polakowski,Julie E. Ledgerwood,Barney S. Graham,Hamilton Bennett,Rolando Pajon,Conor Knightly,Brett Leav,Weiping Deng,Honghong Zhou,Shu Liang Han,Melanie Ivarsson,Jacqueline Miller,Tal Z Zaks +36 more
Antibody resistance of SARS-CoV-2 variants B.1.351 and B.1.1.7.
Pengfei Wang,Manoj S. Nair,Lihong Liu,Sho Iketani,Sho Iketani,Yang Luo,Yicheng Guo,Maple Wang,Jian Yu,Baoshan Zhang,Peter D. Kwong,Peter D. Kwong,Barney S. Graham,John R. Mascola,Jennifer Y Chang,Jennifer Y Chang,Michael T. Yin,Michael T. Yin,Magdalena E. Sobieszczyk,Magdalena E. Sobieszczyk,Christos A. Kyratsous,Lawrence Shapiro,Lawrence Shapiro,Zizhang Sheng,Yaoxing Huang,David D. Ho,David D. Ho +26 more
Convergent antibody responses to SARS-CoV-2 in convalescent individuals.
Davide F. Robbiani,Davide F. Robbiani,Christian Gaebler,Frauke Muecksch,Julio C. C. Lorenzi,Zijun Wang,Alice Cho,Marianna Agudelo,Christopher O. Barnes,Anna Gazumyan,Shlomo Finkin,Thomas Hagglof,Thiago Y. Oliveira,Charlotte Viant,Arlene Hurley,Hans Heinrich Hoffmann,Katrina G. Millard,Rhonda G. Kost,Melissa Cipolla,Kristie Gordon,Filippo Bianchini,Spencer T. Chen,Victor A. Ramos,Roshni Patel,Juan Dizon,Irina Shimeliovich,Pilar Mendoza,Harald Hartweger,Lilian Nogueira,Maggi Pack,Jill Horowitz,Fabian Schmidt,Yiska Weisblum,Eleftherios Michailidis,Alison W. Ashbrook,Eric Waltari,John E. Pak,Kathryn E. Huey-Tubman,Nicholas Koranda,Pauline R. Hoffman,Anthony P. West,Charles M. Rice,Theodora Hatziioannou,Pamela J. Bjorkman,Paul D. Bieniasz,Paul D. Bieniasz,Marina Caskey,Michel C. Nussenzweig,Michel C. Nussenzweig +48 more