mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.
Zijun Wang,Fabian Schmidt,Yiska Weisblum,Frauke Muecksch,Christopher O. Barnes,Shlomo Finkin,Dennis Schaefer-Babajew,Melissa Cipolla,Christian Gaebler,Jenna Ariel Lieberman,Thiago Y. Oliveira,Zhi Yang,Morgan E. Abernathy,Kathryn E. Huey-Tubman,Arlene Hurley,Martina Turroja,Kamille A. West,Kristie Gordon,Katrina G. Millard,Victor A. Ramos,Justin Da Silva,Jianliang Xu,Robert A. Colbert,Roshni Patel,Juan Dizon,Cecille Unson-O'Brien,Irina Shimeliovich,Anna Gazumyan,Marina Caskey,Pamela J. Bjorkman,Rafael Casellas,Theodora Hatziioannou,Paul D. Bieniasz,Paul D. Bieniasz,Michel C. Nussenzweig,Michel C. Nussenzweig +35 more
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This paper reported on the antibody and memory B-cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer-BioNTech (BNT162b2) vaccine against SARS-CoV-2.Abstract:
Here we report on the antibody and memory B cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer–BioNTech (BNT162b2) vaccine against SARS-CoV-21–4 Eight weeks after the second injection of vaccine, volunteers showed high levels of IgM and IgG anti-SARS-CoV-2 spike protein (S) and receptor-binding-domain (RBD) binding titre Moreover, the plasma neutralizing activity and relative numbers of RBD-specific memory B cells of vaccinated volunteers were equivalent to those of individuals who had recovered from natural infection5,6 However, activity against SARS-CoV-2 variants that encode E484K-, N501Y- or K417N/E484K/N501-mutant S was reduced by a small—but significant—margin The monoclonal antibodies elicited by the vaccines potently neutralize SARS-CoV-2, and target a number of different RBD epitopes in common with monoclonal antibodies isolated from infected donors5–8 However, neutralization by 14 of the 17 most-potent monoclonal antibodies that we tested was reduced or abolished by the K417N, E484K or N501Y mutation Notably, these mutations were selected when we cultured recombinant vesicular stomatitis virus expressing SARS-CoV-2 S in the presence of the monoclonal antibodies elicited by the vaccines Together, these results suggest that the monoclonal antibodies in clinical use should be tested against newly arising variants, and that mRNA vaccines may need to be updated periodically to avoid a potential loss of clinical efficacy The Moderna (mRNA-1273) and Pfizer–BioNTech (BNT162b2) vaccines elicit anti-RBD antibodies similar to those elicited through natural infection with SARS-CoV-2, but their potent neutralizing activity was reduced or abolished by new viral variants of concernread more
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SARS-CoV-2 Omicron boosting induces de novo B cell response in humans
Wafaa B. Alsoussi,Sameer Kumar Malladi,Julian Q. Zhou,Zhuoming Li,Baoling Ying,Wooseob Kim,Aaron J. Schmitz,Tingting Lei,Stephen Horvath,Alexandria J. Sturtz,Katherine M. McIntire,Birk Evavold,Fangjie Han,Suzanne M. Scheaffer,Isabella F. Fox,L. Parra-Rodriguez,Raffael Nachbagauer,Biliana Nestorova,Spyros Chalkias,Christopher W Farnsworth,Michael K. Klebert,Iskra Pusic,Benjamin S. Strnad,William D. Middleton,Sharlene A. Teefey,Sean P. J. Whelan,Michael S. Diamond,R. Paris,Jane A. O’Halloran,Rachel M. Presti,Jackson S. Turner,Ali H. Ellebedy +31 more
TL;DR: The authors showed that boosting with an mRNA vaccine against the original monovalent SARS-CoV-2 mRNA vaccine or the bivalent B.1.1-4.617.2 (Beta/Delta) mRNA vaccine induced robust spike-specific germinal centre B cell responses in humans.
Journal ArticleDOI
Altered Local Interactions and Long-Range Communications in UK Variant (B.1.1.7) Spike Glycoprotein
Stefano Borocci,Carmen Cerchia,Alessandro Grottesi,Nico Sanna,Ingrid Guarnetti Prandi,Nabil Abid,Andrea R. Beccari,Giovanni Chillemi,Carmine Talarico +8 more
TL;DR: In this article, the impact of the mutations of the S glycoprotein in SARS-CoV-2 variant of concern 202012/01 (B.1.7), termed the UK variant, in comparison with the wild type, with the aim to decipher the structural basis of the reported increased infectivity and virulence.
Journal ArticleDOI
There is nothing exempt from the peril of mutation – The Omicron spike
Tapan Behl,Ishnoor Kaur,Aayush Sehgal,Sukhbir Singh,Neelam Sharma,Md. Khalid Anwer,Hafiz A. Makeen,Mohammed Al-Bratty,Hassan A. Alhazmi,Saurabh Bhatia,Simona Bungau +10 more
TL;DR: In this paper , the authors provide a holistic picture about the Omicron variant, by providing comprehensive discussions related to multiple aspects of the mutated spike variant, which might aid the global researchers and healthcare experts in finding an optimised solution to this pandemic.
Journal ArticleDOI
Antibody evolution to SARS-CoV-2 after single-dose Ad26.COV2.S vaccine in humans
Alice Cho,Frauke Muecksch,Zhijun Wang,Tarek Ben Tanfous,Justin DaSilva,Raphael Raspe,B J Johnson,Eva Bednarski,Victor Ramos,Dennis Schaefer-Babajew,Irina Shimeliovich,Juan Dizon,Kai-Hui Yao,Fabian Schmidt,Katrina G. Millard,Martina Turroja,Mila Jankovic,Thiago Y. Oliveira,Anna Gazumyan,Christian Gaebler,Marina Caskey,Theodora Hatziioannou,Paul D. Bieniasz,Michel C. Nussenzweig +23 more
TL;DR: The data help explain why boosting Ad.26.COV.2 vaccine recipients with mRNA vaccines is effective, and why the Janssen vaccine appears to have been less protective against severe disease during the Omicron surge than the mRNA vaccine.
Journal ArticleDOI
A dual-antigen self-amplifying RNA SARS-CoV-2 vaccine induces potent humoral and cellular immune responses and protects against SARS-CoV-2 variants through T cell-mediated immunity
Shawn S. McCafferty,A. K. M. Ashiqul Haque,Aster Vandierendonck,Brian Weidensee,Magalie Plovyt,Magdalena Stuchlíková,Nathalie François,Sophie Valembois,Leo Heyndrickx,Johan Michiels,Kevin K. Ariën,Linos Vandekerckhove,Rana Abdelnabi,Caroline S. Foo,Johan Neyts,Itishri Sahu,Niek N. Sanders +16 more
TL;DR: In this article , the authors demonstrate that 1 μg of an LNP-formulated dual-antigen self-amplifying RNA vaccine (ZIP1642), encoding both S-RBD and N antigen, elicits considerably higher neutralizing antibody titers against Wuhan-like Beta B.1.351 and Delta B.617.2 SARS-CoV-2 variants compared to those of convalescent patients.
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