mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.
Zijun Wang,Fabian Schmidt,Yiska Weisblum,Frauke Muecksch,Christopher O. Barnes,Shlomo Finkin,Dennis Schaefer-Babajew,Melissa Cipolla,Christian Gaebler,Jenna Ariel Lieberman,Thiago Y. Oliveira,Zhi Yang,Morgan E. Abernathy,Kathryn E. Huey-Tubman,Arlene Hurley,Martina Turroja,Kamille A. West,Kristie Gordon,Katrina G. Millard,Victor A. Ramos,Justin Da Silva,Jianliang Xu,Robert A. Colbert,Roshni Patel,Juan Dizon,Cecille Unson-O'Brien,Irina Shimeliovich,Anna Gazumyan,Marina Caskey,Pamela J. Bjorkman,Rafael Casellas,Theodora Hatziioannou,Paul D. Bieniasz,Paul D. Bieniasz,Michel C. Nussenzweig,Michel C. Nussenzweig +35 more
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This paper reported on the antibody and memory B-cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer-BioNTech (BNT162b2) vaccine against SARS-CoV-2.Abstract:
Here we report on the antibody and memory B cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer–BioNTech (BNT162b2) vaccine against SARS-CoV-21–4 Eight weeks after the second injection of vaccine, volunteers showed high levels of IgM and IgG anti-SARS-CoV-2 spike protein (S) and receptor-binding-domain (RBD) binding titre Moreover, the plasma neutralizing activity and relative numbers of RBD-specific memory B cells of vaccinated volunteers were equivalent to those of individuals who had recovered from natural infection5,6 However, activity against SARS-CoV-2 variants that encode E484K-, N501Y- or K417N/E484K/N501-mutant S was reduced by a small—but significant—margin The monoclonal antibodies elicited by the vaccines potently neutralize SARS-CoV-2, and target a number of different RBD epitopes in common with monoclonal antibodies isolated from infected donors5–8 However, neutralization by 14 of the 17 most-potent monoclonal antibodies that we tested was reduced or abolished by the K417N, E484K or N501Y mutation Notably, these mutations were selected when we cultured recombinant vesicular stomatitis virus expressing SARS-CoV-2 S in the presence of the monoclonal antibodies elicited by the vaccines Together, these results suggest that the monoclonal antibodies in clinical use should be tested against newly arising variants, and that mRNA vaccines may need to be updated periodically to avoid a potential loss of clinical efficacy The Moderna (mRNA-1273) and Pfizer–BioNTech (BNT162b2) vaccines elicit anti-RBD antibodies similar to those elicited through natural infection with SARS-CoV-2, but their potent neutralizing activity was reduced or abolished by new viral variants of concernread more
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Intramuscular mRNA BNT162b2 vaccine against SARS-CoV-2 induces neutralizing salivary IgA
Miri Stolovich-Rain,Sujata Kumari,Ahuva Friedman,S.O. Kirillov,Yakov Socol,M. Billan,Ritesh Ranjan Pal,Kathakali Das,P R Golding,Esther Oiknine-Djian,Salim Sirhan,Michal Bejerano Sagie,Einav Cohen-Kfir,Naama Gold,Jamal Fahoum,Rajesh Kumar,Maya Elgrably-Weiss,Bing Zhou,Miriam Ravins,Yair E. Gatt,Saurabh Bhattacharya,Orly Zelig,Reuven Wiener,Dana G. Wolf,Hila Elinav,Jacob Strahilevitz,Dan Padawer,Lea Baraz,Alexander Rouvinski +28 more
TL;DR: In this paper , a temporary neutralizing mucosal component of immunity, emanating from intramuscular administration of an mRNA vaccine, was found to associate with the secretory component, indicating their bona fide transcytotic origin.
Journal ArticleDOI
Editorial: Innate immune responses to SARS-CoV-2 in infected and vaccinated individuals
TL;DR: Sena, Reche, Bonam and Mancini as discussed by the authors published an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), provided the original author(s) and the copyright owner(s are credited and that the original publication in this journal is cited, in accordance with accepted academic practice.
Journal ArticleDOI
Fc engineered ACE2-Fc is a potent multifunctional agent targeting SARS-CoV2
Bruce D. Wines,Liriye Kurtovic,Halina M. Trist,Sandra Esparon,Ester Lopez,Klasina Chappin,Li-Jin Chan,Francesca L Mordant,Wen Shi Lee,Nicholas A Gherardin,Sheila K Patel,Gemma E. Hartley,Phillip Pymm,James P Cooney,James G. Beeson,Dale I. Godfrey,Louise M Burrell,Menno C. van Zelm,Adam K. Wheatley,Amy W. Chung,Wai-Hong Tham,Kanta Subbarao,Stephen J. Kent,P. Mark Hogarth +23 more
TL;DR: The capacity of Fc-based agents to be engineered to optimize different mechanisms of protection for SARS-CoV-2 and potentially other viral pathogens is demonstrated.
Posted ContentDOI
Antibody Attributes that Predict the Neutralization and Effector Function of Polyclonal Responses to SARS-CoV-2
Harini Natarajan,Shiwei Xu,Andrew R. Crowley,Ssavannah E. Butler,Joshua A. Weiner,Evan M. Bloch,Kirsten Littlefield,Sarah E. Benner,Ruchee Shrestha,Olivia Ajayi,Wendy F. Weiland-Alter,David J. Sullivan,Shmuel Shoham,Thomas C. Quinn,Thomas C. Quinn,Arturo Casadevall,Andrew Pekosz,Andrew D. Redd,Andrew D. Redd,Aaron A.R. Tobian,Ruth I. Connor,Peter F. Wright,Margaret E. Ackerman +22 more
TL;DR: In this paper, the authors employ machine learning to relate characteristics of the polyclonal antibody response raised by natural infection to diverse antibody effector functions and neutralization potency with the goal of generating both accurate predictions of each activity based on antibody response profiles as well as insights into antibody mechanisms of action.
Posted ContentDOI
Atypical N-glycosylation of SARS-CoV-2 impairs the efficient binding of Spike-RBM to the human-host receptor hACE2
Gustavo Gámez,Juan A. Hermoso,César Carrasco-López,Alejandro Gómez-Mejia,Carlos Muskus,Sven Hammerschmidt +5 more
TL;DR: In this article, the authors presented a study that was funded by CELSIA and ISA (Interconexion Electrica S.A.) in Colombia and supported by the======BFU2017-90030-P grant to J.H.A.
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