mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.
Zijun Wang,Fabian Schmidt,Yiska Weisblum,Frauke Muecksch,Christopher O. Barnes,Shlomo Finkin,Dennis Schaefer-Babajew,Melissa Cipolla,Christian Gaebler,Jenna Ariel Lieberman,Thiago Y. Oliveira,Zhi Yang,Morgan E. Abernathy,Kathryn E. Huey-Tubman,Arlene Hurley,Martina Turroja,Kamille A. West,Kristie Gordon,Katrina G. Millard,Victor A. Ramos,Justin Da Silva,Jianliang Xu,Robert A. Colbert,Roshni Patel,Juan Dizon,Cecille Unson-O'Brien,Irina Shimeliovich,Anna Gazumyan,Marina Caskey,Pamela J. Bjorkman,Rafael Casellas,Theodora Hatziioannou,Paul D. Bieniasz,Paul D. Bieniasz,Michel C. Nussenzweig,Michel C. Nussenzweig +35 more
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TLDR
This paper reported on the antibody and memory B-cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer-BioNTech (BNT162b2) vaccine against SARS-CoV-2.Abstract:
Here we report on the antibody and memory B cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer–BioNTech (BNT162b2) vaccine against SARS-CoV-21–4 Eight weeks after the second injection of vaccine, volunteers showed high levels of IgM and IgG anti-SARS-CoV-2 spike protein (S) and receptor-binding-domain (RBD) binding titre Moreover, the plasma neutralizing activity and relative numbers of RBD-specific memory B cells of vaccinated volunteers were equivalent to those of individuals who had recovered from natural infection5,6 However, activity against SARS-CoV-2 variants that encode E484K-, N501Y- or K417N/E484K/N501-mutant S was reduced by a small—but significant—margin The monoclonal antibodies elicited by the vaccines potently neutralize SARS-CoV-2, and target a number of different RBD epitopes in common with monoclonal antibodies isolated from infected donors5–8 However, neutralization by 14 of the 17 most-potent monoclonal antibodies that we tested was reduced or abolished by the K417N, E484K or N501Y mutation Notably, these mutations were selected when we cultured recombinant vesicular stomatitis virus expressing SARS-CoV-2 S in the presence of the monoclonal antibodies elicited by the vaccines Together, these results suggest that the monoclonal antibodies in clinical use should be tested against newly arising variants, and that mRNA vaccines may need to be updated periodically to avoid a potential loss of clinical efficacy The Moderna (mRNA-1273) and Pfizer–BioNTech (BNT162b2) vaccines elicit anti-RBD antibodies similar to those elicited through natural infection with SARS-CoV-2, but their potent neutralizing activity was reduced or abolished by new viral variants of concernread more
Citations
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Injectable Slow-Release Hydrogel Formulation of a Plant Virus-Based COVID-19 Vaccine Candidate
Christian Isalomboto Nkanga,Oscar A Ortega-Rivera,Matthew D Shin,Miguel A Moreno-Gonzalez,Nicole F. Steinmetz +4 more
TL;DR: It is concluded that CPMV nanoparticles can be formulated effectively in chitosan/GP hydrogels and are released as intact particles for several months with conserved immunotherapeutic efficacy.
Journal ArticleDOI
Cross-neutralization of SARS-CoV-2 Kappa and Delta variants by inactivated vaccine-elicited serum and monoclonal antibodies.
Lin Cheng,Shuo Song,Qing Fan,Senlin Shen,Haiyan Wang,Bing Zhou,Xiangyang Ge,Bin Ju,Zheng Zhang,Zheng Zhang +9 more
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Determination of the Concentration of IgG against the Spike Receptor-Binding Domain That Predicts the Viral Neutralizing Activity of Convalescent Plasma and Serum against SARS-CoV-2.
Llipsy Santiago,Iratxe Uranga-Murillo,Maykel Arias,Andrés Manuel González-Ramírez,Javier Macías-León,Eduardo Moreo,Sergio Redrado,Ana García-García,Víctor Taleb,Erandi Lira-Navarrete,Ramon Hurtado-Guerrero,Nacho Aguilo,Maria del Mar Encabo-Berzosa,Sandra Hidalgo,Eva M. Galvez,Ariel Ramirez-Labrada,Diego de Miguel,Rafael Benito,Rafael Benito,Patricia Miranda,Antonio Fernández,José María Domingo,Laura Serrano,Cristina Yuste,Sergio Villanueva-Saz,José Ramón Paño-Pardo,Julián Pardo +26 more
TL;DR: In this article, the authors used an in-house quantitative ELISA test to find that plasma from 177 convalescent donors contained IgG antibodies specific to the spike receptor-binding domain (RBD) of SARS-CoV-2, although at very different concentrations which correlated with previous disease severity and gender.
Journal ArticleDOI
Neutralising reactivity against SARS-CoV-2 Delta and Omicron variants by vaccination and infection history
Enrico Lavezzo,Monia Pacenti,Laura Manuto,Caterina Boldrin,Margherita Cattai,Marco Grazioli,Federico A. Bianca,M.S. Sartori,Federico Caldart,Gioele Castelli,Michele Nicoletti,Eleonora Nieddu,Elisa Salvadoretti,Beatrice Labella,Ludovico Fava,Maria Cristina Vanuzzo,Vittoria Lisi,Maria Antonello,C. Grimaldi,Chiara Zulian,Claudia Del Vecchio,Mario Plebani,Andrea Padoan,Daniela Maria Cirillo,Alessandra Rosalba Brazzale,Giovanni Tonon,Stefano Toppo,Ilaria Dorigatti,Andrea Crisanti +28 more
TL;DR: In this article , the authors investigated the long-term antibody persistence in previously infected subjects and the extent of the antibody-mediated protection against B.1, B.2 and BA.1.
Posted ContentDOI
Effects of Spike Mutations in SARS-CoV-2 Variants of Concern on Human or Animal ACE2-Mediated Virus Entry and Neutralization
Yunjeong Kim,Natasha N. Gaudreault,David A. Meekins,Krishani Dinali Perera,Dashzeveg Bold,Jessie D. Trujillo,Igor Morozov,Chester McDowell,Kyeong-Ok Chang,Juergen A. Richt +9 more
TL;DR: In this paper, the effect of mutations in the SARS-CoV-2 Spike protein (S) has been investigated in nine different animal species to gain insights into the effects of VOC mutations on viral entry and antibody neutralization capability.
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