Multi-site assessment of rapid, point-of-care antigen testing for the diagnosis of SARS-CoV-2 infection in a low-prevalence setting: A validation and implementation study
Stephen Muhi,Stephen Muhi,Nick Tayler,Nick Tayler,Tuyet Hoang,Susan A Ballard,Maryza Graham,Amanda Rojek,Jason C Kwong,Jason C Kwong,Jason A Trubiano,Olivia C Smibert,George P Drewett,Fiona L James,Emma Gardiner,Socheata Chea,Nicole Isles,Michelle Sait,Shivani Pasricha,George Taiaroa,Julie L. McAuley,Eloise Williams,Katherine B Gibney,Katherine B Gibney,Timothy P. Stinear,Katherine Bond,Katherine Bond,Sharon R Lewin,Sharon R Lewin,Sharon R Lewin,Mark Putland,Benjamin P Howden,Benjamin P Howden,Deborah A Williamson,Deborah A Williamson +34 more
- Vol. 9, pp 100115-100115
TLDR
In this paper, the authors evaluated the performance and implementation of rapid antigen-based SARS-CoV-2 testing in a setting with a low prevalence of COVID-19 infections, such as Australia.Abstract:
Background: In Australia, COVID-19 diagnosis relies on RT-PCR testing which is relatively costly and time-consuming To date, few studies have assessed the performance and implementation of rapid antigen-based SARS-CoV-2 testing in a setting with a low prevalence of COVID-19 infections, such as Australia Methods: This study recruited participants presenting for COVID-19 testing at three Melbourne metropolitan hospitals during a period of low COVID-19 prevalence The Abbott PanBioTM COVID-19 Ag point-of-care test was performed alongside RT-PCR In addition, participants with COVID-19 notified to the Victorian Government were invited to provide additional swabs to aid validation Implementation challenges were also documented Findings: The specificity of the Abbott PanBioTM COVID-19 Ag test was 9996% (95% CI 9973 - 100%) Sensitivity amongst participants with RT-PCR-confirmed infection was dependent upon the duration of symptoms reported, ranging from 773% (duration 1 to 33 days) to 100% in those within seven days of symptom onset A range of implementation challenges were identified which may inform future COVID-19 testing strategies in a low prevalence setting Interpretation: Given the high specificity, antigen-based tests may be most useful in rapidly triaging public health and hospital resources while expediting confirmatory RT-PCR testing Considering the limitations in test sensitivity and the potential for rapid transmission in susceptible populations, particularly in hospital settings, careful consideration is required for implementation of antigen testing in a low prevalence setting Funding: This work was funded by the Victorian Department of Health and Human Services The funder was not involved in data analysis or manuscript preparationread more
Citations
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Posted ContentDOI
A point-of-care lateral flow assay for neutralising antibodies against SARS-CoV-2
Thomas S Fulford,Huy Van,Nicholas A Gherardin,Nicholas A Gherardin,Shuning Zheng,Ciula M,Heidi E. Drummer,Heidi E. Drummer,Heidi E. Drummer,Samuel J. Redmond,Hyon-Xhi Tan,Fan Li,Samantha L. Grimley,Bruce D. Wines,Bruce D. Wines,Bruce D. Wines,Thi H. O. Nguyen,Francesca L Mordant,Louise C. Rowntree,Allen C. Cheng,Doola Dl,Katherine Bond,P.M. Hogarth,P.M. Hogarth,P.M. Hogarth,Zoe McQuilten,Kanta Subbarao,Katherine Kedzierska,Jennifer A Juno,Wheatley Ak,Wheatley Ak,Stephen J. Kent,Stephen J. Kent,Stephen J. Kent,Deborah A Williamson,Deborah A Williamson,Damian F. J. Purcell,David A. Anderson,Dale I. Godfrey,Dale I. Godfrey +39 more
TL;DR: A lateral flow POC test that can measure levels of RBD-ACE2 neutralizing antibodies from whole blood, with a result that can be determined by eye (semi-quantitative) or on a small instrument (quantitative), and results show high correlation with microneutralization assays as discussed by the authors.
Journal ArticleDOI
Simultaneous monitoring of eight human respiratory viruses including SARS-CoV-2 using liquid chromatography-tandem mass spectrometry
Christopher Hodgkins,Laura K. Buckton,Gregory J. Walker,Ben Crossett,Stuart J. Cordwell,Andrea R. Horvath,William D. Rawlinson +6 more
TL;DR: In this paper , the authors developed a fast "bottom-up" proteomics workflow for discovery of target virus peptides using "serum-free" culture conditions, providing high coverage of viral proteins without the need for protein or peptide fractionation techniques.
Journal ArticleDOI
Keeping kids in school: modelling school-based testing and quarantine strategies during the COVID-19 pandemic in Australia
Romesh G. Abeysuriya,Rachel Sacks-Davis,Katherine Heath,Dominic Delport,Fiona M. Russell,Margie Danchin,Margaret Hellard,Jodie McVernon,Nick Scott +8 more
TL;DR: In this article , a stochastic agent-based model of COVID-19 transmission was used to evaluate a test-to-stay strategy using daily rapid antigen tests (RATs) for close contacts of a case for 7 days compared with home quarantine; and an asymptomatic surveillance strategy involving twice-weekly screening of all students and/or teachers using RATs.
Journal ArticleDOI
Engaging with communities to encourage adoption of a harm reduction approach to COVID-19
TL;DR: In this paper , the authors provide a perspective on the support and guidance required to mitigate individuals and communities risk by using a harm reduction approach to highlight strategies that can reduce COVID-19 transmission and infection.
Book ChapterDOI
Point-of-Care Testing of COVID-19: Current Status, Clinical Impact, and Future Therapeutic Perspectives
TL;DR: In this article , a review of point-of-care (POC) tests for the coronavirus disease 2019 (COVID-19) pandemic is presented, focusing on recent advances in the field.
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