Journal ArticleDOI
Mutagenesis of the Bacillus subtilis "-12, -24" promoter of the levanase operon and evidence for the existence of an upstream activating sequence.
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TLDR
The drastic down effect of base substitutions in the TGGCAC, TTGCA consensus sequence on the expression of the levanase operon confirmed the involvement of the "-12, -24" region in promoter function and the isolation and characterization of eight mutations in the UAS region confirmed the importance of the palindromic structure in promoter activation.About:
This article is published in Journal of Molecular Biology.The article was published on 1992-07-05. It has received 77 citations till now. The article focuses on the topics: Upstream activating sequence & Levanase.read more
Citations
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Journal ArticleDOI
Genetic regulation of nitrogen fixation in rhizobia.
TL;DR: Although the involvement of FixLJ and FixK in nifA regulation is remarkably different in the three rhizobial species discussed here, they constitute a regulatory cascade that uniformly controls the expression of genes (fixNOQP) encoding a distinct cytochrome oxidase complex probably required for bacterial respiration under low-oxygen conditions.
Journal ArticleDOI
Regulation of carbon catabolism in Bacillus species.
Jörg Stülke,Wolfgang Hillen +1 more
TL;DR: The gram-positive bacterium Bacillus subtilisis capable of using numerous carbohydrates as single sources of carbon and energy is discussed, with antitermination apparently more common in B. subtil is than in other bacteria.
Journal ArticleDOI
Compilation and analysis of sigma(54)-dependent promoter sequences.
TL;DR: The use of the extended consensus increases the probability of identifying genuine -24/-12 promoters and the effect of several reported mutations on RNA-polymerase binding and promoter strength is discussed in the light of the updated consensus.
Journal ArticleDOI
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression
Anne Galinier,Jacques Haiech,Marie-Claude Kilhoffer,Michel Jaquinod,Jörg Stülke,Josef Deutscher,Isabelle Martin-Verstraete +6 more
TL;DR: The results suggest that CCR of certain catabolic operons requires, in addition to CcpA, ATP-dependent phosphorylation of Crh, and HPr at Ser-46, as well as the discovery of a new B. subtilis gene encoding a HPr-like protein, Crh (for catabolite repression HPr).
Journal ArticleDOI
Fermentative metabolism of Bacillus subtilis: physiology and regulation of gene expression.
Hugo Cruz Ramos,Tamara Hoffmann,Tamara Hoffmann,Tamara Hoffmann,Marco Marino,Hafed Nedjari,Elena Presecan-Siedel,Oliver Dreesen,Philippe Glaser,Dieter Jahn +9 more
TL;DR: In agreement with its known aerobic function, no obvious oxygen- or nitrate-dependent pta regulation was observed and a model for the regulation of the anaerobic fermentation genes in B. subtilis is proposed.
References
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Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
Rapid and efficient site-specific mutagenesis without phenotypic selection.
TL;DR: The high efficiency, approximately equal to 10-fold greater than that observed using current methods without enrichment procedures, is obtained by using a DNA template containing several uracil residues in place of thymine, which is applied to mutations introduced via both oligonucleotides and error-prone polymerization.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Book ChapterDOI
Rapid and efficient site-specific mutagenesis without phenotypic selection
Journal ArticleDOI
Requirements for transformation in bacillus subtilis.
C. Anagnostopoulos,John Spizizen +1 more
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