Neuropeptide S is a stimulatory anxiolytic agent: a behavioural study in mice
Anna Rizzi,Raffaella Vergura,Giuliano Marzola,Chiara Ruzza,Remo Guerrini,Severo Salvadori,Domenico Regoli,Girolamo Calo +7 more
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TLDR
The effects of in vivo supraspinal NPS in mice are evaluated and it is shown that in vivo NPS produces a unique behavioural profile by increasing wakefulness and exerting anxiolytic‐like effects.Abstract:
Background and purpose:
Neuropeptide S (NPS) was recently identified as the endogenous ligand of an orphan receptor, now referred to as the NPS receptor. In vivo, NPS produces a unique behavioural profile by increasing wakefulness and exerting anxiolytic-like effects. In the present study, we further evaluated the effects of in vivo supraspinal NPS in mice.
Experimental approach:
Effects of NPS, injected intracerebroventricularly (i.c.v.), on locomotor activity (LA), righting reflex (RR) recovery and on anxiety states (measured with the elevated plus maze (EPM) and stress-induced hyperthermia (SIH) tests) were assessed in Swiss mice.
Key results:
NPS (0.01–1 nmol per mouse) caused a significant increase in LA in naive mice, in mice habituated to the test cages and in animals sedated with diazepam (5 mg kg−1). In the RR assay, NPS dose dependently reduced the proportion of animals losing the RR in response to diazepam (15 mg kg−1) and their sleeping time. In the EPM and SIH test, NPS dose dependently evoked anxiolytic-like effects by increasing the time spent by animals in the open arms and reducing the SIH response, respectively.
Conclusions and implications:
We provide further evidence that NPS acts as a novel modulator of arousal and anxiety-related behaviours by promoting a unique pattern of effects: stimulation associated with anxiolysis. Therefore, NPS receptor ligands may represent innovative drugs for the treatment of sleep and anxiety disorders.
British Journal of Pharmacology (2008) 154, 471–479; doi:10.1038/bjp.2008.96; published online 31 March 2008read more
Citations
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Neuropeptide S receptor gene -- converging evidence for a role in panic disorder.
Katharina Domschke,Andreas Reif,Heike Weber,Jan Richter,Christa Hohoff,Patricia Ohrmann,Anya Pedersen,Jochen Bauer,Thomas Suslow,Harald Kugel,Walter Heindel,Christian Baumann,Benedikt Klauke,Christian Jacob,Wolfgang Maier,Jürgen Fritze,Borwin Bandelow,Petra Krakowitzky,Matthias Rothermundt,Angelika Erhardt,Elisabeth B. Binder,Florian Holsboer,Alexander L. Gerlach,Tilo Kircher,Thomas Lang,Thomas Lang,Georg W. Alpers,Georg W. Alpers,Andreas Ströhle,Lydia Fehm,Andrew T. Gloster,Hans-Ulrich Wittchen,Volker Arolt,Paul Pauli,Alfons O. Hamm,Jürgen Deckert +35 more
TL;DR: In this article, a multilevel approach was applied to further elucidate the role of neuropeptide S in the etiology of human anxiety, and the functional NPSR A/T (Asn 107 Ile) variant (rs324981) was investigated for association with panic disorder.
Journal ArticleDOI
Neuropeptide S: A transmitter system in the brain regulating fear and anxiety
TL;DR: The recently discovered Neuropeptide S and its cognate receptor represent a highly interesting system of neuromodulation with unique physiological effects that produces anxiolytic-like effects by acutely reducing fear responses as well as modulating long-term aspects of fear memory.
Journal ArticleDOI
Nasal application of neuropeptide S reduces anxiety and prolongs memory in rats: social versus non-social effects.
Michael Lukas,Inga D. Neumann +1 more
TL;DR: Naval application of NPS seems to be a useful method in rodents for screening for behavioral or physiological effects before more specific and time-consuming, intracerebral methods are employed, and may represent a viable therapeutic approach for NPS treatment of patients with psychiatric illnesses such as anxiety or panic disorders.
Journal ArticleDOI
Transcriptomic identification of starfish neuropeptide precursors yields new insights into neuropeptide evolution
Dean C. Semmens,Olivier Mirabeau,Ismail Moghul,Mahesh R. Pancholi,Yannick Wurm,Maurice R. Elphick +5 more
TL;DR: This is the most comprehensive identification of neuropeptide precursor proteins in an echinoderm to date, yielding new insights into the evolution of neuroPEptide signalling systems.
Journal ArticleDOI
Neuropeptide S enhances memory during the consolidation phase and interacts with noradrenergic systems in the brain
Naoe Okamura,Celia Garau,Dee M. Duangdao,Stewart D. Clark,Kay Jüngling,Hans-Christian Pape,Rainer K. Reinscheid +6 more
TL;DR: Evidence is provided for a facilitatory role of NPS in long-term memory, independent of memory content, possibly by acting as a salience signal or as an arousal-promoting factor.
References
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Journal ArticleDOI
Central injections of nocistatin or its C-terminal hexapeptide exert anxiogenic-like effect on behaviour of mice in the plus-maze test.
Elaine C. Gavioli,Giles A. Rae,Girolamo Calo,Remo Guerrini,Thereza Christina Monteiro de Lima +4 more
TL;DR: Results reveal potent anxiogenic‐like actions of NST and NST‐C6, and confirm the anxiolytic‐like properties of N/OFQ, as anxiety may be modulated in opposing directions depending on how this precursor is processed.
Journal ArticleDOI
Expression and function of NPSR1/GPRA in the lung before and after induction of asthma-like disease
Irving C. Allen,Amy J. Pace,Leigh A. Jania,Julie G. Ledford,Anne M. Latour,John N. Snouwaert,Virginie Bernier,Rino Stocco,Alex G. Therien,Beverly H. Koller +9 more
TL;DR: It is reported here that the development of allergic lung disease in GPRA-deficient mice is unaltered, and the data suggest that GPRA may contribute to the asthmatic phenotype by altering the activity of other pathways, such as neurally mediated mechanisms, that contribute to disease.
Journal ArticleDOI
Centrally administered neuropeptide S activates orexin-containing neurons in the hypothalamus and stimulates feeding in rats
TL;DR: The results suggest that arousal and feeding induced by NPS in the central nervous system may be related to the activation of orexin-expressing neurons.
Journal ArticleDOI
Neuropeptide S and G protein-coupled receptor 154 modulate macrophage immune responses
Ville Pulkkinen,Marja-Leena Majuri,Guoying Wang,P. Holopainen,Yasushi Obase,Johanna Vendelin,Henrik Wolff,Paula Rytilä,Lauri A. Laitinen,Tari Haahtela,Tarja Laitinen,Harri Alenius,Juha Kere,Marko Rehn +13 more
TL;DR: The results show that GPR154 is upregulated in macrophages after antigen challenge and that NPS is capable of inducing phagocytosis of unopsonized bacteria.
Journal ArticleDOI
[Arg14,Lys15]Nociceptin, a Highly Potent Agonist of the Nociceptin/Orphanin FQ Receptor: in Vitro and in Vivo Studies
Daniela Rizzi,Anna Rizzi,Raffaella Bigoni,Valeria Camarda,Giuliano Marzola,Remo Guerrini,Carmela De Risi,Domenico Regoli,Girolamo Calo +8 more
TL;DR: The present data demonstrate that [Arg14,Lys15]NC behaves as a highly potent agonist of the OP4 receptor and is able to produce long-lasting effects in vivo, compared with the natural ligand NC.