Pseudomonas fluorescens F113 mutant with enhanced competitive colonization ability and improved biocontrol activity against fungal root pathogens.
Emma Barahona,Ana Navazo,Francisco Martínez-Granero,Teresa Zea-Bonilla,Rosa María Pérez-Jiménez,Marta Martín,Rafael Rivilla +6 more
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It is shown that the kinB gene, which encodes a signal transduction protein that together with AlgB has been implicated in alginate production, participates in swimming motility repression through the GAC pathway, acting downstream of the GacAS two-component system.Abstract:
Motility is one of the most important traits for efficient rhizosphere colonization by Pseudomonas fluorescens F113rif (F113). In this bacterium, motility is a polygenic trait that is repressed by at least three independent pathways, including the Gac posttranscriptional system, the Wsp chemotaxis-like pathway, and the SadB pathway. Here we show that the kinB gene, which encodes a signal transduction protein that together with AlgB has been implicated in alginate production, participates in swimming motility repression through the Gac pathway, acting downstream of the GacAS two-component system. Gac mutants are impaired in secondary metabolite production and are unsuitable as biocontrol agents. However, the kinB mutant and a triple mutant affected in kinB, sadB, and wspR (KSW) possess a wild-type phenotype for secondary metabolism. The KSW strain is hypermotile and more competitive for rhizosphere colonization than the wild-type strain. We have compared the biocontrol activity of KSW with those of the wild-type strain and a phenotypic variant (F113v35 [V35]) which is hypermotile and hypercompetitive but is affected in secondary metabolism since it harbors a gacS mutation. Biocontrol experiments in the Fusarium oxysporum f. sp. radicis-lycopersici/Lycopersicum esculentum (tomato) and Phytophthora cactorum/Fragaria vesca (strawberry) pathosystems have shown that the three strains possess biocontrol activity. Biocontrol activity was consistently lower for V35, indicating that the production of secondary metabolites was the most important trait for biocontrol. Strain KSW showed improved biocontrol compared with the wild-type strain, indicating that an increase in competitive colonization ability resulted in improved biocontrol and that the rational design of biocontrol agents by mutation is feasible.read more
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Mechanisms of action of plant growth promoting bacteria
TL;DR: To realize the objective of worldwide sustainable agriculture, it is essential that the many mechanisms employed by PGPB first be thoroughly understood thereby allowing workers to fully harness the potentials of these microbes.
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Mining Halophytes for Plant Growth-Promoting Halotolerant Bacteria to Enhance the Salinity Tolerance of Non-halophytic Crops.
Hassan Etesami,Gwyn A. Beattie +1 more
TL;DR: These studies illustrate that halophilic PGPRs from the rhizosphere of halophytic species can be effective bio-inoculants for promoting the production of non-halophyticspecies in saline soils and support the viability of bioinoculation with halophiles as a strategy for the sustainable enhancement ofNon-Halophytic crop growth.
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Regulation of biofilm formation in Pseudomonas and Burkholderia species
Mustafa Fazli,Mustafa Fazli,Henrik Almblad,Morten Rybtke,Michael Givskov,Michael Givskov,Leo Eberl,Tim Tolker-Nielsen +7 more
TL;DR: Although the three regulatory systems all regulate the production of factors used for biofilm formation, the molecular mechanisms involved in transducing the signals into expression of the biofilm matrix components differ between the species.
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Microbiology, Genomics, and Clinical Significance of the Pseudomonas fluorescens Species Complex, an Unappreciated Colonizer of Humans
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Microbial Inoculants for Improving Crop Quality and Human Health in Africa.
TL;DR: The impact of agricultural chemical inputs on human health is summarized and microbial inoculants have the ability to minimize the negative impact of chemical input and consequently increase the quantity and quality of farm produce.
References
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