R2R3-MYB gene pairs in Populus: evolution and contribution to secondary wall formation and flowering time.
Guohua Chai,Zengguang Wang,Xianfeng Tang,Li Yu,Guang Qi,Dian Wang,Xiaofei Yan,Yingzhen Kong,Gongke Zhou +8 more
TLDR
The characterization of 81 pairs of Populus R2R3-MYB genes showed that these gene pairs resulted from multiple types of gene duplications and had five different gene fates.Abstract:
In plants, the R2R3-MYB gene family contains many pairs of paralogous genes, which play the diverse roles in developmental processes and environmental responses. The paper reports the characterization of 81 pairs of Populus R2R3-MYB genes. Chromosome placement, phylogenetic, and motif structure analyses showed that these gene pairs resulted from multiple types of gene duplications and had five different gene fates. Tissue expression patterns revealed that most duplicated genes were specifically expressed in the tissues examined. qRT-PCR confirmed that nine pairs were highly expressed in xylem, of which three pairs (PdMYB10/128, PdMYB90/167, and PdMYB92/125) were further functionally characterized. The six PdMYBs were localized to the nucleus and had transcriptional activities in yeast. The heterologous expression of PdMYB10 and 128 in Arabidopsis increased stem fibre cell-wall thickness and delayed flowering. In contrast, overexpression of PdMYB90, 167, 92, and 125 in Arabidopsis decreased stem fibre and vessel cell-wall thickness and promoted flowering. Cellulose, xylose, and lignin contents were changed in overexpression plants. The expression levels of several genes involved in secondary wall formation and flowering were affected by the overexpression of the six PdMYBs in Arabidopsis. This study addresses the diversity of gene duplications in Populus R2R3-MYBs and the roles of these six genes in secondary wall formation and flowering control.read more
Citations
More filters
Journal ArticleDOI
Genetic modification of plant cell walls to enhance biomass yield and biofuel production in bioenergy crops.
TL;DR: A novel groove-like cell wall model is proposed that highlights to increase amorphous regions (density and depth) of the native cellulose microfibrils, providing a general strategy for bioenergy crop breeding and biofuel processing technology.
Journal ArticleDOI
Two R2R3-MYB proteins are broad repressors of flavonoid and phenylpropanoid metabolism in poplar.
TL;DR: Two new repressor MYBs are characterized, poplar MYB165 and MYB194, paralogs which comprise a subgroup of R2R3-MYBs distinct from previously reported poplar repressors, and may also affect the shikimate pathway.
Journal ArticleDOI
Recent Advances in the Transcriptional Regulation of Secondary Cell Wall Biosynthesis in the Woody Plants.
TL;DR: This article synthesizes recent progress on the transcriptional regulation of SCW biosynthesis in Arabidopsis and contrast to what is known in woody species and evaluates progress in related emerging regulatory machineries targeting transcription factors in this complex regulatory network of SCw biosynthesis.
Journal ArticleDOI
PtoMYB92 is a Transcriptional Activator of the Lignin Biosynthetic Pathway During Secondary Cell Wall Formation in Populus tomentosa.
TL;DR: Subcellular localization and transcriptional activation analysis suggest that PtoMYB92 is a nuclear-localized transcriptional activator in the lignin biosynthetic pathway during secondary cell wall formation in poplar by controlling the biosynthesis of monolignols.
Journal ArticleDOI
Arabidopsis C3H14 and C3H15 have overlapping roles in the regulation of secondary wall thickening and anther development
Guohua Chai,Yingzhen Kong,Ming Zhu,Li Yu,Guang Qi,Xianfeng Tang,Zengguang Wang,Yingping Cao,Changjiang Yu,Gongke Zhou +9 more
TL;DR: It is demonstrated here that C3H14 and its homologue C3h15 redundantly regulate secondary wall formation and that they additionally function in anther development.
References
More filters
Journal ArticleDOI
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Naruya Saitou,Masatoshi Nei +1 more
TL;DR: The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.
Journal ArticleDOI
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
TL;DR: ClUSTAL X is a new windows interface for the widely-used progressive multiple sequence alignment program CLUSTAL W, providing an integrated system for performing multiple sequence and profile alignments and analysing the results.
Journal ArticleDOI
A new mathematical model for relative quantification in real-time RT-PCR.
TL;DR: This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript and presents a new mathematical model that needs no calibration curve.
Journal ArticleDOI
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
TL;DR: Version 4 of MEGA software expands on the existing facilities for editing DNA sequence data from autosequencers, mining Web-databases, performing automatic and manual sequence alignment, analyzing sequence alignments to estimate evolutionary distances, inferring phylogenetic trees, and testing evolutionary hypotheses.
Journal ArticleDOI
The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)
Gerald A. Tuskan,Gerald A. Tuskan,Stephen P. DiFazio,Stephen P. DiFazio,Stefan Jansson,Joerg Bohlmann,Igor V. Grigoriev,Uffe Hellsten,Nicholas H. Putnam,Steven G. Ralph,Stephane Rombauts,Asaf Salamov,Jacquie Schein,Lieven Sterck,Andrea Aerts,Rishikeshi Bhalerao,Rishikesh P. Bhalerao,Damien Blaudez,Wout Boerjan,Annick Brun,Amy M. Brunner,Victor Busov,Malcolm M. Campbell,John E. Carlson,Michel Chalot,Jarrod Chapman,G.-L. Chen,Dawn Cooper,Pedro M. Coutinho,Jérémy Couturier,Sarah F. Covert,Quentin C. B. Cronk,R. Cunningham,John M. Davis,Sven Degroeve,Annabelle Déjardin,Claude W. dePamphilis,John C. Detter,Bill Dirks,Inna Dubchak,Inna Dubchak,Sébastien Duplessis,Jürgen Ehlting,Brian E. Ellis,Karla C Gendler,David Goodstein,Michael Gribskov,Jane Grimwood,Andrew Groover,Lee E. Gunter,Björn Hamberger,Berthold Heinze,Yrjö Helariutta,Yrjö Helariutta,Yrjö Helariutta,Bernard Henrissat,D. Holligan,Robert A. Holt,Wenyu Huang,N. Islam-Faridi,Steven J.M. Jones,M. Jones-Rhoades,Richard A. Jorgensen,Chandrashekhar P. Joshi,Jaakko Kangasjärvi,Jan Karlsson,Colin T. Kelleher,Robert Kirkpatrick,Matias Kirst,Annegret Kohler,Udaya C. Kalluri,Frank W. Larimer,Jim Leebens-Mack,Jean-Charles Leplé,Philip F. LoCascio,Y. Lou,Susan Lucas,Francis Martin,Barbara Montanini,Carolyn A. Napoli,David R. Nelson,C D Nelson,Kaisa Nieminen,Ove Nilsson,V. Pereda,Gary F. Peter,Ryan N. Philippe,Gilles Pilate,Alexander Poliakov,J. Razumovskaya,Paul G. Richardson,Cécile Rinaldi,Kermit Ritland,Pierre Rouzé,D. Ryaboy,Jeremy Schmutz,J. Schrader,Bo Segerman,H. Shin,Asim Siddiqui,Fredrik Sterky,Astrid Terry,Chung-Jui Tsai,Edward C. Uberbacher,Per Unneberg,Jorma Vahala,Kerr Wall,Susan R. Wessler,Guojun Yang,T. Yin,Carl J. Douglas,Marco A. Marra,Göran Sandberg,Y. Van de Peer,Daniel S. Rokhsar,Daniel S. Rokhsar +115 more
TL;DR: The draft genome of the black cottonwood tree, Populus trichocarpa, has been reported in this paper, with more than 45,000 putative protein-coding genes identified.