Journal ArticleDOI
Recent advances in systems metabolic engineering tools and strategies.
TLDR
Recent advances in systems metabolic engineering tools and strategies are reviewed together with accompanying application examples and how these tools and Strategies work together in simultaneous and synergistic ways to develop novel microbial cell factories are described.About:
This article is published in Current Opinion in Biotechnology.The article was published on 2017-10-01. It has received 173 citations till now. The article focuses on the topics: Systems biology.read more
Citations
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Journal ArticleDOI
A comprehensive metabolic map for production of bio-based chemicals
Sang Yup Lee,Hyun Uk Kim,Tong Un Chae,Jae Sung Cho,Je Woong Kim,Jae Ho Shin,Dong In Kim,Ko Yoo Sung,Woo Dae Jang,Yu Sin Jang +9 more
TL;DR: This Review provides a comprehensive overview of biological and chemical routes for the synthesis of industrial chemicals derived from key precursor metabolites of central carbon metabolic pathways, and visualizes the results in a global bio-based chemicals map.
Journal ArticleDOI
Systems Metabolic Engineering Strategies: Integrating Systems and Synthetic Biology with Metabolic Engineering
TL;DR: Current trends in systems metabolic engineering including tools and strategies are reviewed, focusing on recent developments in selection of host strains, metabolic pathway reconstruction, tolerance enhancement, and metabolic flux optimization.
Journal ArticleDOI
An automated Design-Build-Test-Learn pipeline for enhanced microbial production of fine chemicals
Pablo Carbonell,Adrian J. Jervis,Christopher J. Robinson,Cunyu Yan,Mark S. Dunstan,Neil Swainston,Maria Vinaixa,Katherine A. Hollywood,Andrew Currin,Nicholas J. W. Rattray,Sandra Taylor,Reynard Spiess,Rehana Sung,Alan Williams,Donal Fellows,Natalie J. Stanford,Paul Mulherin,Rosalind Le Feuvre,Perdita E. Barran,Royston Goodacre,Nicholas J. Turner,Carole Goble,George Guo-Qiang Chen,George Guo-Qiang Chen,Douglas B. Kell,Jason Micklefield,Rainer Breitling,Eriko Takano,Jean-Loup Faulon,Jean-Loup Faulon,Nigel S. Scrutton +30 more
TL;DR: An automated pipeline for the discovery and optimization of biosynthetic pathways for microbial production of fine chemicals is presented and application of the pipeline to optimize an alkaloids pathway demonstrates how it could facilitate the rapid optimization of microbial strains for production of any chemical compound of interest.
Journal ArticleDOI
Advances and prospects of Bacillus subtilis cellular factories: From rational design to industrial applications.
TL;DR: This review proposed emerging opportunities and essential strategies to enable the successful development of B. subtilis as microbial cell factories as well as summarized the recent advances in systems and synthetic biology, engineering strategies for improving cellular performances, and metabolic engineering applications of B.'s subtillis.
Journal ArticleDOI
High-Level dCas9 Expression Induces Abnormal Cell Morphology in Escherichia coli
TL;DR: Results support the fact that dCas9 has critical effects on cell division as well as inner and outer membrane structure, and the regulation of intracellular levels of d Cas9 is pivotal to avoid unexpected morphological changes in E. coli.
References
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Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
TL;DR: This strategy uses the host’s highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis to achieve high-yield, high-specificity production of isobutanol from glucose.
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High-level semi-synthetic production of the potent antimalarial artemisinin
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TL;DR: The strains and processes described here form the basis of a viable industrial process for the production of semi-synthetic artemisinin to stabilize the supply of art Artemisinin for derivatization into active pharmaceutical ingredients (for example, artesunate) for incorporation into ACTs.
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Programming cells by multiplex genome engineering and accelerated evolution
Harris H. Wang,Farren J. Isaacs,Peter A. Carr,Zachary Z. Sun,George Xu,Craig R. Forest,George M. Church +6 more
TL;DR: The multiplex approach embraces engineering in the context of evolution by expediting the design and evolution of organisms with new and improved properties by facilitating rapid and continuous generation of a diverse set of genetic changes.