Replication-Transcription Conflicts Generate R-Loops that Orchestrate Bacterial Stress Survival and Pathogenesis
Kevin S. Lang,Ashley N. Hall,Christopher N. Merrikh,Mark N. Ragheb,Hannah Tabakh,Alex J. Pollock,Joshua J. Woodward,Julia E. Dreifus,Houra Merrikh +8 more
TLDR
It is shown that pervasive R-loop formation at head-on collision regions completely blocks replication, elevates mutagenesis, and inhibits gene expression, and that the resolution of these structures is critical for bacterial stress survival and pathogenesis.About:
This article is published in Cell.The article was published on 2017-08-10 and is currently open access. It has received 176 citations till now. The article focuses on the topics: Control of chromosome duplication & DNA replication.read more
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R-Loops as Cellular Regulators and Genomic Threats.
TL;DR: This review highlights recent work suggesting that R-loops can be problematic to cells as blocks to efficient transcription and replication that trigger the DNA damage response and compares the available next-generation sequencing-based approaches to map R-loop genome wide.
Journal ArticleDOI
R Loops: From Physiological to Pathological Roles
TL;DR: Given the R-loop impact on chromatin and genome organization and its potential relation with genetic diseases, this work reviews R- loop homeostasis as well as their physiological and pathological roles.
Journal ArticleDOI
Mechanisms of Oncogene-Induced Replication Stress: Jigsaw Falling into Place.
TL;DR: The latest findings on the DNA replication stress response are discussed and the various mechanisms through which activated oncogenes induce replication stress are examined, which may provide new avenues for targeted cancer treatment.
Journal ArticleDOI
Regulatory R-loops as facilitators of gene expression and genome stability.
Christof Niehrs,Brian Luke +1 more
TL;DR: The cellular processes that regulatory R-loops modulate, the regulation of R-Loops and the potential differences that may exist may be discussed, including transcription termination and other steps of gene regulation, telomere stability and DNA repair.
Journal ArticleDOI
The DNA damage response to transcription stress.
TL;DR: TBL detection by the stalling of RNA polymerase II is highly efficient but may interfere with repair, and overall with transcription and replication, Consequently, TC-NER deregulation causes hereditary disorders with complex genotype–phenotype correlations.
References
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Journal ArticleDOI
The Sequence Alignment/Map format and SAMtools
Heng Li,Bob Handsaker,Alec Wysoker,T. J. Fennell,Jue Ruan,Nils Homer,Gabor T. Marth,Gonçalo R. Abecasis,Richard Durbin +8 more
TL;DR: SAMtools as discussed by the authors implements various utilities for post-processing alignments in the SAM format, such as indexing, variant caller and alignment viewer, and thus provides universal tools for processing read alignments.
Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
deepTools: a flexible platform for exploring deep-sequencing data
TL;DR: A Galaxy based web server for processing and visualizing deeply sequenced data, called deepTools, that enables users with little bioinformatic background to explore the results of their sequencing experiments in a standardized setting and can be used without registration.
Book
Molecular biological methods for Bacillus
TL;DR: Part 1 Growth maintenance and general techniques, C.R.Harwood and A.Archibald: the genus Bacillus growth of Bacillus maintenance and shipping of strains cell permeabilization and breakage radiolabelling of macromolecules.
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