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Open AccessJournal ArticleDOI

SCPD: a promoter database of the yeast Saccharomyces cerevisiae.

Jian Zhu, +1 more
- Vol. 15, Iss: 7, pp 607-611
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TLDR
A comprehensive yeast-specific promoter database that contains relevant binding affinity and expression data where available and provides some simple but useful tools for promoter sequence analysis is developed.
Abstract
MOTIVATION: In order to facilitate a systematic study of the promoters and transcriptionally regulatory cis-elements of the yeast Saccharomyces cerevisiae on a genomic scale, we have developed a comprehensive yeast-specific promoter database, SCPD. RESULTS: Currently SCPD contains 580 experimentally mapped transcription factor (TF) binding sites and 425 transcriptional start sites (TSS) as its primary data entries. It also contains relevant binding affinity and expression data where available. In addition to mechanisms for promoter information (including sequence) retrieval and a data submission form, SCPD also provides some simple but useful tools for promoter sequence analysis. AVAILABILITY: SCPD can be accessed from the URL http://cgsigma.cshl.org/jian. The database is continually updated.

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TL;DR: The results provide a preview for how expression profiles in multi-cellular eukaryotes might be impacted by NMD, and the methods for analyzing decay rates on a global scale offer a blueprint for new ways to study mRNA decay pathways in any organism where cultured cell lines are available.
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Computational identification of transcriptional regulatory elements in DNA sequence

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Fast and systematic genome-wide discovery of conserved regulatory elements using a non-alignment based approach.

TL;DR: A powerful new approach for discovering globally conserved regulatory elements between two genomes is described, which is fast, simple and comprehensive, without requiring alignments.
References
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Journal ArticleDOI

Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale

TL;DR: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.
Journal ArticleDOI

The role of general initiation factors in transcription by RNA polymerase II.

TL;DR: Transcription initiation on protein-encoding genes represents a major control point for gene expression in eukaryotes, and is mediated by RNA polymerase II and a surprisingly complex array of general initiation factors that are highly conserved from yeast to man.
Journal ArticleDOI

SGD: Saccharomyces Genome Database

TL;DR: The Saccharomyces Genome Database (SGD) provides Internet access to the complete SacCharomyces cerevisiae genomic sequence, its genes and their products, the phenotypes of its mutants, and the literature supporting these data.
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