Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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TLDR
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
Citations
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Journal ArticleDOI
Recent insights into the genotype-phenotype relationship from massively parallel genetic assays.
TL;DR: The technological basis of and recent developments in massively parallel genetics, along with the trends it is uncovering in the genotype–phenotype relationship (distribution of mutation effects, epistasis), their possible mechanistic bases and future directions for advancing towards the goal of predictive genetics are discussed.
Journal ArticleDOI
Monoclonal antibodies that recognise filamentous phage: tools for phage display technology.
Luciana Dente,Gianni Cesareni,Gioacchino Micheli,Franco Felici,Antonella Folgori,Alessandra Luzzago,Paolo Monaci,Alfredo Nicosia,Paola Delmastro +8 more
TL;DR: Six hybridoma cell lines that secrete monoclonal antibodies which specifically bind filamentous phage coat proteins are generated, which are valuable tools to study phage assembly and structure and how these mAb can be exploited in the construction and screening of peptide libraries displayed by the filamentous phase major coat protein.
Journal ArticleDOI
Next-generation protein drugs.
TL;DR: Ankyrin repeats generate high-affinity protein binders with biophysical properties that may favor therapeutic applications and be a source of inspiration for new drug candidates.
Patent
Stabilized liquid anti-rsv antibody formulations
TL;DR: In this paper, the authors provide liquid formulations of SYNAGIS or an antigen-binding fragment thereof that immunospecifically bind to a respiratory syncytial virus (RSV) antigen.
Journal ArticleDOI
Preparation of peptides which mimic glycosphingolipids by using phage peptide library and their modulation on β‐galactosidase activity
TL;DR: This is the first report of carbohydrate mimicking peptides which modulate glycosidase activity and binding activity of the peptides to the monoclonal antibodies or Ricinus communis lectin showed that 9‐mer peptides are enough to mimic the epitope carbohydrate structure.
References
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Journal ArticleDOI
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