Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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TLDR
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
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Journal ArticleDOI
Interaction and co-encapsidation of human immunodeficiency virus type 1 Gag and Vif recombinant proteins
Isabelle Huvent,Saw See Hong,C. Fournier,Jeannette Tournier,Christian Carrière,Marianne Courcoul,Robert Vigne,Bruno Spire,Pierre Boulanger +8 more
TL;DR: The data suggest a direct intracellular Gag-Vif interaction and the occurrence of a Pr55Gag-mediated membrane-targeting pathway for Vif in Sf9 cells.
Journal ArticleDOI
Peptides targeting caspase inhibitors.
Ingo Tamm,Martin Trepel,Marina Cardó-Vila,Yan Sun,Kate Welsh,Edelmira Cabezas,Arnold Swatterthwait,Wadih Arap,John C. Reed,Renata Pasqualini +9 more
TL;DR: It is demonstrated that an internalizing version of the XIAP-binding peptide identified in the authors' screenings (PFKQ) can induce programmed cell death in leukemia cells.
Patent
Method of selection of proteolytic cleavage sites by directed evolution and phagemid display
TL;DR: In this article, a method for identifying and selecting novel substrates for enzymes is provided, which comprises constructing a gene fusion comprising DNA encoding a polypeptide fused to DNA encoding at least a portion of a phage coat protein.
Journal ArticleDOI
Designing scaffolds of peptides for phage display libraries.
TL;DR: It is found that the conformational design of peptides in library is important for selecting high-affinity ligands that bind to every target from a phage peptide library.
Journal ArticleDOI
Identification of epitopes within beta lactoglobulin recognised by polyclonal antibodies using phage display and PEPSCAN
TL;DR: This study has permitted detailed mapping of the major linear antigenic regions within BLG recognised by IgG antibodies from immunised rabbits and mice.
References
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Journal ArticleDOI
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