Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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TLDR
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
Citations
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Journal ArticleDOI
Phage antibodies: will new ‘coliclonal’ antibodies replace monoclonal antibodies?
David J. Chiswell,John McCaffery +1 more
TL;DR: Antibodies can now be rapidly isolated from large and diverse recombinant libraries by displaying functional antibody fragments on the surface of bacteriophage particles and directly selecting with antigen.
Journal ArticleDOI
High affinity binding of monoclonal antibodies to the sequential epitope EFRH of β-amyloid peptide is essential for modulation of fibrillar aggregation
TL;DR: While mAbs 6C6 and 10D5 inhibit the formation of beta-amyloid fibrils, trigger disaggregation and reversal to its non-toxic form, mAb 2H3 is devoid of these properties.
Patent
Method of screening a peptide library
Lam Kit S,Sydney E. Salmon +1 more
TL;DR: The instant invention as discussed by the authors provides a library of bio-oligomers of defined size and known composition, in which the library contains all of the possible sequences of the bio-OLigomers, and a method of synthesis thereof.
Journal ArticleDOI
High-affinity binding of short peptides to major histocompatibility complex class II molecules by anchor combinations.
Juergen Hammer,Charles Belunis,David Robert Bolin,Joanne Papadopoulos,Robert L. Walsky,Jacqueline Higelin,Waleed Danho,Francesco Sinigaglia,Zoltan A. Nagy +8 more
TL;DR: A peptide-binding motif has been obtained, in which six of seven positions show enrichment of certain residues, and the anchor at relative position 1 seems to be obligatory, in that its substitution abrogates binding completely, whereas the elimination of other anchors results in partial loss of binding affinity.
Patent
Human growth hormone variant having enhanced affinity for human growth hormone receptor at site 1
TL;DR: In this paper, a human growth hormone variant including the set of amino acid substitutions H18A, Q22A, F25A, D26A, E29A and E65A was disclosed.
References
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Journal ArticleDOI
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