Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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TLDR
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
Citations
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Journal ArticleDOI
Peptide phage libraries can be an efficient tool for identifying antibody ligands for polyclonal antisera.
TL;DR: The data demonstrate the potential of random peptide phage libraries for defining epitopes for polyclonal antisera as well as for investigation of the nature of B cell responses to any given antigen.
Journal ArticleDOI
Delineation of a linear epitope by multiple peptide synthesis and phage display.
Laurence Choulier,Daniel Laune,Georges Orfanoudakis,Hanna Wlad,Jan-Christer Janson,Claude Granier,Danièle Altschuh +6 more
TL;DR: The results show that the functional epitope of Fab 57P requires precise physico-chemical properties at a limited number of positions, and that residues flanking these key residues can influence binding affinity.
Journal ArticleDOI
Identification of HLA-DR9 (DRB1*0901)-binding peptide motifs using a phage fUSE5 random peptide library
TL;DR: Collectively, DR9-binding peptide motifs identified in this study are characteristic in that only two anchors of the NH2-terminal half of binding peptides play important roles in binding, and small neutral hydrophilic Ser is allowed as the second anchor for high-affinity binding, unlike the other DR-binding motifs heretofore reported.
Journal ArticleDOI
Specificity of Interactions among the DNA-packaging Machine Components of T4-related Bacteriophages
Song Gao,Venigalla B. Rao +1 more
TL;DR: Analysis of interactions between small and large terminases of T4-related phages leads to a model in which multiple weak interactions between motor and regulator allow dynamic assembly and disassembly of various packaging complexes, depending on the functional state of the packaging machine.
Patent
Immunizing composition and method for inducing an immune response against the ss-secretase cleavage site of amyloid precursor protein
TL;DR: In this paper, an immunizing composition containing an antigenic product such as a multiple antigen peptide system (MAPS) or a filamentous bacteriophage displaying an AβPP epitope spanning the β-secretase cleavage site of A βPP and a method for inducing an immune response against the β secret enzyme cleavage sites was presented.
References
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