Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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TLDR
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
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Journal ArticleDOI
Exploration of the HIF-1α/p300 interface using peptide and Adhiron phage display technologies
Hannah F. Kyle,Kate F. Wickson,Jonathan Stott,George M. Burslem,Alexander L. Breeze,Christian Tiede,Darren C. Tomlinson,Stuart L. Warriner,Adam Nelson,Andrew J. Wilson,Thomas A. Edwards +10 more
TL;DR: A detailed biophysical analysis of the native interaction of the HIF-1α/p300 protein-protein interaction with both peptide and Adhiron phage display experiments is reported to identify novel binding motifs and binding regions of the surface of p300 to inform future inhibitor design.
Journal ArticleDOI
In situ click chemistry: from small molecule discovery to synthetic antibodies
Steven W. Millward,Heather D. Agnew,Bert T. Lai,Su Seong Lee,Jaehong Lim,Arundhati Nag,Suresh M. Pitram,Rosemary D. Rohde,James R. Heath +8 more
TL;DR: This review presents a review of Iterative Peptide In Situ Click Chemistry (IPISC), a novel screening technology for designing peptide multiligands with high affinity and specificity, and discusses the recent progress in ligand design through IPISC and related approaches.
Patent
A structured peptide scaffold for displaying turn libraries on phage
TL;DR: In this paper, a model system for structure-activity analysis of peptide or protein molecules involved in important biological processes is presented, which are combinatorial peptide libraries comprising disulfide-constrained cyclic peptides.
Patent
Inhibition of stat3 signal transduction for human cancer therapy
Richard Jove,William S. Dalton,Said M. Sebti,Hua Yu,Richard Heller,Mark J. Jaroszeski,Richard Gilbert,Andrew D. Hamilton +7 more
TL;DR: Signal Transducer and Activator of Transcription (STAT) proteins have a fundamental role cell signaling, and are activated by a large number of cytokines and growth factors as discussed by the authors.
Journal ArticleDOI
Tumour suppressor genes and molecular chaperones
TL;DR: The p53 protein interacts with members of the hsp70 chaperone family which it is shown can regulate its function, leading to an attractive model of p53 as an essential checkpoint control.
References
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