Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
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A complex of α6 integrin and E‐cadherin drives liver metastasis of colorectal cancer cells through hepatic angiopoietin‐like 6
Serena Marchiò,Marco Soster,Sabrina Cardaci,Andrea Muratore,Alice Bartolini,Vanessa Barone,Dario Ribero,Maria Chiara Monti,Paola Bovino,Jessica Sun,Raffaella Giavazzi,Sofia Asioli,Paola Cassoni,Lorenzo Capussotti,Piero Pucci,Antonella Bugatti,Marco Rusnati,Renata Pasqualini,Wadih Arap,Federico Bussolino +19 more
TL;DR: It is demonstrated that an interaction between hepatic angiopoietin‐like 6 and tumoural α6 integrin/E‐cadherin drives liver homing and colonization by CRC cells, and that CGIYRLRSC inhibits liver metastasis through interference with this ligand/receptor system.
Journal ArticleDOI
New molecular biology methods for protein engineering
TL;DR: This review outlines recent advances in the application of molecular biological techniques to the study of protein structure and function through methods for oligonucleotide-directed mutagenesis; mutational strategies for identifying functional residues and domains; systems for expression; and future developments.
Journal ArticleDOI
An in vitro selection strategy for conferring protease resistance to ligand binding peptides
Bill Eldridge,R. Neil Cooley,Richard Odegrip,Duncan Mcgregor,Kevin Fitzgerald,Christopher Ullman +5 more
TL;DR: In vitro display technology, CIS display, is used to enhance the proteolytic resistance of ligand-binding peptides by selection of protecting motifs from a large peptide library, which translated into increased resistance to plasma proteases in vitro and to an increase in circulating half-lives in rats.
Journal ArticleDOI
Identification of Soft Matter Binding Peptide Ligands Using Phage Display.
Kemal Arda Günay,Harm-Anton Klok +1 more
TL;DR: The article will provide an overview of the different peptide ligands that have been identified by phage display that bind to these "soft matter" targets and the different characterization techniques that allow the determination of the affinity of the identified ligands to the respective substrates.
Journal ArticleDOI
Ligand discovery using small molecule microarrays.
TL;DR: In this review, some of the technical issues related to the manufacture and screening of small molecule microarrays, as well as prospects for small moleculemicroarrays in several areas of drug discovery and chemistry, are discussed.
References
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Journal ArticleDOI
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